Molecular cloning and characterization of two isoforms of cyclophilin A gene from Venerupis philippinarum
Chen, Leilei1; Mu, Changkao1; Zhao, Jianmin2; Wang, Chunlin1
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
2011-12-01
Volume31Issue:6Pages:1218-1223
KeywordVenerupis Philippinarum Cyclophilin a Gene Expression
Contribution Rank[Chen, Leilei; Mu, Changkao; Wang, Chunlin] Ningbo Univ, Fac Life Sci & Biotechnol, Ningbo 315211, Zhejiang, Peoples R China; [Zhao, Jianmin] Chinese Acad Sci, Key Lab Coastal Zone Environm Proc, Shandong Prov Key Lab Coastal Zone Environm Proc, CAS,Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China
Corresponding AuthorWang, CL (reprint author), Ningbo Univ, Fac Life Sci & Biotechnol, Ningbo 315211, Zhejiang, Peoples R China
Department污染过程与控制实验室 
AbstractCyclophilin A (CypA) is a ubiquitously distributed intracellular protein belonging to the immunophilin family, which is recognized as the cell receptor for the potent immunosuppressive drug cyclosporine A. In the present study, two isoforms of cyclophilin A gene (named as VpCypA1 and VpCypA2) were isolated and characterized from Venerupis philippinarum by RACE approaches. Both VpCypA1 and VpCypA2 possessed all conserved features critical for the fundamental structure and function of CypA, indicating that the two isoforms of cyclophilin A should be new members of CypA family. The expression of VpCypA2 mRNA in haemocytes was significantly up-regulated and the highest expression level was detected at 96 h post-infection with 7.7-fold increase compared with that in the blank group. On the contrary, the relative expression level of VpCypA1 mRNA was down-regulated rapidly at 6 h post-infection and reached 0.4-fold of the control group. They exhibited different expression profile and identical effect of immune modulation, which might suggest the two VpCypA isoforms exert their function in a manner of synergy. These results provide valuable information for further exploring the roles of cyclophilin A in the immune responses of V. philippinarum. (C) 2011 Elsevier Ltd. All rights reserved.; Cyclophilin A (CypA) is a ubiquitously distributed intracellular protein belonging to the immunophilin family, which is recognized as the cell receptor for the potent immunosuppressive drug cyclosporine A. In the present study, two isoforms of cyclophilin A gene (named as VpCypA1 and VpCypA2) were isolated and characterized from Venerupis philippinarum by RACE approaches. Both VpCypA1 and VpCypA2 possessed all conserved features critical for the fundamental structure and function of CypA, indicating that the two isoforms of cyclophilin A should be new members of CypA family. The expression of VpCypA2 mRNA in haemocytes was significantly up-regulated and the highest expression level was detected at 96 h post-infection with 7.7-fold increase compared with that in the blank group. On the contrary, the relative expression level of VpCypA1 mRNA was down-regulated rapidly at 6 h post-infection and reached 0.4-fold of the control group. They exhibited different expression profile and identical effect of immune modulation, which might suggest the two VpCypA isoforms exert their function in a manner of synergy. These results provide valuable information for further exploring the roles of cyclophilin A in the immune responses of V. philippinarum. (C) 2011 Elsevier Ltd. All rights reserved.
Funding OrganizationInnovation Research Team in University[IRT0734]; Ministry of Education of China[20103305110003, 20103305120002]; Nature Fund of Ningbo[2010A610004]; Ningbo University
SubtypeArticle
Indexed BySCI
Language英语
WOS KeywordCIS-TRANS-ISOMERASE ; BINDING-PROTEIN CYCLOPHILIN ; OXIDATIVE STRESS ; ZHIKONG SCALLOP ; CHLAMYS-FARRERI ; CYCLOSPORINE-A ; EXPRESSION ; FAMILY ; IMMUNOPHILINS ; MECHANISM
WOS Research AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS IDWOS:000298569700063
Citation statistics
Cited Times:15[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.yic.ac.cn/handle/133337/5380
Collection中科院海岸带环境过程与生态修复重点实验室_污染过程与控制实验室
Affiliation1.Ningbo Univ, Fac Life Sci & Biotechnol, Ningbo 315211, Zhejiang, Peoples R China
2.Chinese Acad Sci, Key Lab Coastal Zone Environm Proc, Shandong Prov Key Lab Coastal Zone Environm Proc, CAS,Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China
Recommended Citation
GB/T 7714
Chen, Leilei,Mu, Changkao,Zhao, Jianmin,et al. Molecular cloning and characterization of two isoforms of cyclophilin A gene from Venerupis philippinarum[J]. FISH & SHELLFISH IMMUNOLOGY,2011,31(6):1218-1223.
APA Chen, Leilei,Mu, Changkao,Zhao, Jianmin,&Wang, Chunlin.(2011).Molecular cloning and characterization of two isoforms of cyclophilin A gene from Venerupis philippinarum.FISH & SHELLFISH IMMUNOLOGY,31(6),1218-1223.
MLA Chen, Leilei,et al."Molecular cloning and characterization of two isoforms of cyclophilin A gene from Venerupis philippinarum".FISH & SHELLFISH IMMUNOLOGY 31.6(2011):1218-1223.
Files in This Item: Download All
File Name/Size DocType Version Access License
Molecular cloning an(1406KB) 开放获取--View Download
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Chen, Leilei]'s Articles
[Mu, Changkao]'s Articles
[Zhao, Jianmin]'s Articles
Baidu academic
Similar articles in Baidu academic
[Chen, Leilei]'s Articles
[Mu, Changkao]'s Articles
[Zhao, Jianmin]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Chen, Leilei]'s Articles
[Mu, Changkao]'s Articles
[Zhao, Jianmin]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Molecular cloning and characterization of two isoforms of cyclophilin A gene from Venerupis philippinarum.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.