| Molecular cloning and characterization of two isoforms of cyclophilin A gene from Venerupis philippinarum | |
Chen, Leilei1; Mu, Changkao1; Zhao, Jianmin2 ; Wang, Chunlin1
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| 发表期刊 | FISH & SHELLFISH IMMUNOLOGY
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| ISSN | 1050-4648 |
| 2011-12-01 | |
| 卷号 | 31期号:6页码:1218-1223 |
| 关键词 | Venerupis Philippinarum Cyclophilin a Gene Expression |
| 产权排序 | [Chen, Leilei; Mu, Changkao; Wang, Chunlin] Ningbo Univ, Fac Life Sci & Biotechnol, Ningbo 315211, Zhejiang, Peoples R China; [Zhao, Jianmin] Chinese Acad Sci, Key Lab Coastal Zone Environm Proc, Shandong Prov Key Lab Coastal Zone Environm Proc, CAS,Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China |
| 通讯作者 | Wang, CL (reprint author), Ningbo Univ, Fac Life Sci & Biotechnol, Ningbo 315211, Zhejiang, Peoples R China |
| 作者部门 | 污染过程与控制实验室 |
| 英文摘要 | Cyclophilin A (CypA) is a ubiquitously distributed intracellular protein belonging to the immunophilin family, which is recognized as the cell receptor for the potent immunosuppressive drug cyclosporine A. In the present study, two isoforms of cyclophilin A gene (named as VpCypA1 and VpCypA2) were isolated and characterized from Venerupis philippinarum by RACE approaches. Both VpCypA1 and VpCypA2 possessed all conserved features critical for the fundamental structure and function of CypA, indicating that the two isoforms of cyclophilin A should be new members of CypA family. The expression of VpCypA2 mRNA in haemocytes was significantly up-regulated and the highest expression level was detected at 96 h post-infection with 7.7-fold increase compared with that in the blank group. On the contrary, the relative expression level of VpCypA1 mRNA was down-regulated rapidly at 6 h post-infection and reached 0.4-fold of the control group. They exhibited different expression profile and identical effect of immune modulation, which might suggest the two VpCypA isoforms exert their function in a manner of synergy. These results provide valuable information for further exploring the roles of cyclophilin A in the immune responses of V. philippinarum. (C) 2011 Elsevier Ltd. All rights reserved.; Cyclophilin A (CypA) is a ubiquitously distributed intracellular protein belonging to the immunophilin family, which is recognized as the cell receptor for the potent immunosuppressive drug cyclosporine A. In the present study, two isoforms of cyclophilin A gene (named as VpCypA1 and VpCypA2) were isolated and characterized from Venerupis philippinarum by RACE approaches. Both VpCypA1 and VpCypA2 possessed all conserved features critical for the fundamental structure and function of CypA, indicating that the two isoforms of cyclophilin A should be new members of CypA family. The expression of VpCypA2 mRNA in haemocytes was significantly up-regulated and the highest expression level was detected at 96 h post-infection with 7.7-fold increase compared with that in the blank group. On the contrary, the relative expression level of VpCypA1 mRNA was down-regulated rapidly at 6 h post-infection and reached 0.4-fold of the control group. They exhibited different expression profile and identical effect of immune modulation, which might suggest the two VpCypA isoforms exert their function in a manner of synergy. These results provide valuable information for further exploring the roles of cyclophilin A in the immune responses of V. philippinarum. (C) 2011 Elsevier Ltd. All rights reserved. |
| 文章类型 | Article |
| 资助机构 | Innovation Research Team in University[IRT0734]; Ministry of Education of China[20103305110003, 20103305120002]; Nature Fund of Ningbo[2010A610004]; Ningbo University |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | CIS-TRANS-ISOMERASE ; BINDING-PROTEIN CYCLOPHILIN ; OXIDATIVE STRESS ; ZHIKONG SCALLOP ; CHLAMYS-FARRERI ; CYCLOSPORINE-A ; EXPRESSION ; FAMILY ; IMMUNOPHILINS ; MECHANISM |
| 研究领域[WOS] | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| WOS记录号 | WOS:000298569700063 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/5380 |
| 专题 | 中国科学院海岸带环境过程与生态修复重点实验室_污染过程与控制实验室 海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室 |
| 作者单位 | 1.Ningbo Univ, Fac Life Sci & Biotechnol, Ningbo 315211, Zhejiang, Peoples R China 2.Chinese Acad Sci, Key Lab Coastal Zone Environm Proc, Shandong Prov Key Lab Coastal Zone Environm Proc, CAS,Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Leilei,Mu, Changkao,Zhao, Jianmin,et al. Molecular cloning and characterization of two isoforms of cyclophilin A gene from Venerupis philippinarum[J]. FISH & SHELLFISH IMMUNOLOGY,2011,31(6):1218-1223. |
| APA | Chen, Leilei,Mu, Changkao,Zhao, Jianmin,&Wang, Chunlin.(2011).Molecular cloning and characterization of two isoforms of cyclophilin A gene from Venerupis philippinarum.FISH & SHELLFISH IMMUNOLOGY,31(6),1218-1223. |
| MLA | Chen, Leilei,et al."Molecular cloning and characterization of two isoforms of cyclophilin A gene from Venerupis philippinarum".FISH & SHELLFISH IMMUNOLOGY 31.6(2011):1218-1223. |
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