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ANALYSIS OF URINARY PORPHYRINS BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY-ELECTROSPRAY IONIZATION MASS SPECTROMETRY
Li, Jinhua1,2; Cai, Zongwei2; Xu, Shoufang1,4; Liao, Chunyang1; Song, Xingliang3; Chen, Lingxin1
2011
发表期刊JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
ISSN1082-6076
卷号34期号:15页码:1578-1593
产权排序[Li, Jinhua; Xu, Shoufang; Liao, Chunyang; Chen, Lingxin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China; [Li, Jinhua; Cai, Zongwei] Hong Kong Baptist Univ, Dept Chem, Hong Kong, Hong Kong, Peoples R China; [Song, Xingliang] Linyi Univ, Dept Chem, Linyi, Peoples R China; [Xu, Shoufang] Chinese Acad Sci, Grad Univ, Beijing, Peoples R China
摘要A high-performance liquid chromatography coupled with electrospray ionization mass spectrometry (HPLC-ESI-MS) method was developed for the simultaneous separation and detection of six porphyrins, namely uroporphyrin (UP), heptacarboxylic acid porphyrin (HEPTAP), hexacarboxylic acid porphyrin (HEXAP), pentacarboxylic acid porphyrin (PENTAP), coproporphyrin (CP), and mesoporphyrin (MP), in human urine samples. The recoveries of the porphyrins in urine ranged from 84 to 108%. The intra-and inter-day precisions at spiked porphyrin concentrations were 4.0-9.7% and 5.5-15%, respectively. The limits of detection (LOD) ranged from 0.2 to 3 nM. The developed method was successfully applied to the determination of endogenous porphyrins in human urine. UP, HEPTAP, and PENTAP were detected at levels of 42.4, 21.3, and 18.0 nM, respectively. CP was detected as the predominant species at 135.6 nM, wheres MP and HEXAP were not detected in the urine samples from healthy females. Significant increases of CP excretion in urine from liver disease patients were observed. This method proved potentially applicable for the routine diagnosis and treatment of porphyrin-related diseases.; A high-performance liquid chromatography coupled with electrospray ionization mass spectrometry (HPLC-ESI-MS) method was developed for the simultaneous separation and detection of six porphyrins, namely uroporphyrin (UP), heptacarboxylic acid porphyrin (HEPTAP), hexacarboxylic acid porphyrin (HEXAP), pentacarboxylic acid porphyrin (PENTAP), coproporphyrin (CP), and mesoporphyrin (MP), in human urine samples. The recoveries of the porphyrins in urine ranged from 84 to 108%. The intra-and inter-day precisions at spiked porphyrin concentrations were 4.0-9.7% and 5.5-15%, respectively. The limits of detection (LOD) ranged from 0.2 to 3 nM. The developed method was successfully applied to the determination of endogenous porphyrins in human urine. UP, HEPTAP, and PENTAP were detected at levels of 42.4, 21.3, and 18.0 nM, respectively. CP was detected as the predominant species at 135.6 nM, wheres MP and HEXAP were not detected in the urine samples from healthy females. Significant increases of CP excretion in urine from liver disease patients were observed. This method proved potentially applicable for the routine diagnosis and treatment of porphyrin-related diseases.
通讯作者Chen, LX (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China
关键词Electrospray Ionization High Performance Liquid Chromatography Human Urine Mass Spectrometry Porphyrins Solid-phase Extraction
作者部门环境化学实验室 
学科领域Biochemistry & Molecular Biology ; Chemistry
项目资助者National Natural Science Foundation of China[20975089, 20907039]; Department of Science and Technology of Shandong Province of China[2008GG20005005, 2010GSF10222]; Natural Science Foundation of Shandong Province of China[Y2007B38]; Yantai Research and Development Program[2007323]; Chinese Academy of Sciences
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收录类别SCI
关键词[WOS]CAPILLARY-ELECTROPHORESIS ; ELECTROCHEMICAL DETECTION ; FLUORESCENCE DETECTION ; HPLC METHOD ; SEPARATION ; EXTRACTION ; DIAGNOSIS ; STACKING ; ISOMERS ; CLEANUP
文章类型Article
语种英语
WOS研究方向Biochemistry & Molecular Biology ; Chemistry
WOS记录号WOS:000296231000009
引用统计
被引频次:8[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cn/handle/133337/5327
专题环境化学实验室
作者单位1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China
2.Hong Kong Baptist Univ, Dept Chem, Hong Kong, Hong Kong, Peoples R China
3.Linyi Univ, Dept Chem, Linyi, Peoples R China
4.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China
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GB/T 7714
Li, Jinhua,Cai, Zongwei,Xu, Shoufang,et al. ANALYSIS OF URINARY PORPHYRINS BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY-ELECTROSPRAY IONIZATION MASS SPECTROMETRY[J]. JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,2011,34(15):1578-1593.
APA Li, Jinhua,Cai, Zongwei,Xu, Shoufang,Liao, Chunyang,Song, Xingliang,&Chen, Lingxin.(2011).ANALYSIS OF URINARY PORPHYRINS BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY-ELECTROSPRAY IONIZATION MASS SPECTROMETRY.JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,34(15),1578-1593.
MLA Li, Jinhua,et al."ANALYSIS OF URINARY PORPHYRINS BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY-ELECTROSPRAY IONIZATION MASS SPECTROMETRY".JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES 34.15(2011):1578-1593.
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