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题名:
ANALYSIS OF URINARY PORPHYRINS BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY-ELECTROSPRAY IONIZATION MASS SPECTROMETRY
作者: Jinhua LI(李金花)1,2; Cai, Zongwei2; Xu, Shoufang1,4; Liao, Chunyang1; Song, Xingliang3; Chen, Lingxin1
刊名: JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
ISSN号: 1082-6076
出版日期: 2011
卷号: 34, 期号:15, 页码:1578-1593
关键词: electrospray ionization ; high performance liquid chromatography ; human urine ; mass spectrometry ; porphyrins ; solid-phase extraction
学科分类: Biochemistry & Molecular Biology; Chemistry
产权排序: [Li, Jinhua; Xu, Shoufang; Liao, Chunyang; Chen, Lingxin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China; [Li, Jinhua; Cai, Zongwei] Hong Kong Baptist Univ, Dept Chem, Hong Kong, Hong Kong, Peoples R China; [Song, Xingliang] Linyi Univ, Dept Chem, Linyi, Peoples R China; [Xu, Shoufang] Chinese Acad Sci, Grad Univ, Beijing, Peoples R China
通讯作者: Chen, LX (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China
作者部门: 环境化学实验室 
中文摘要: A high-performance liquid chromatography coupled with electrospray ionization mass spectrometry (HPLC-ESI-MS) method was developed for the simultaneous separation and detection of six porphyrins, namely uroporphyrin (UP), heptacarboxylic acid porphyrin (HEPTAP), hexacarboxylic acid porphyrin (HEXAP), pentacarboxylic acid porphyrin (PENTAP), coproporphyrin (CP), and mesoporphyrin (MP), in human urine samples. The recoveries of the porphyrins in urine ranged from 84 to 108%. The intra-and inter-day precisions at spiked porphyrin concentrations were 4.0-9.7% and 5.5-15%, respectively. The limits of detection (LOD) ranged from 0.2 to 3 nM. The developed method was successfully applied to the determination of endogenous porphyrins in human urine. UP, HEPTAP, and PENTAP were detected at levels of 42.4, 21.3, and 18.0 nM, respectively. CP was detected as the predominant species at 135.6 nM, wheres MP and HEXAP were not detected in the urine samples from healthy females. Significant increases of CP excretion in urine from liver disease patients were observed. This method proved potentially applicable for the routine diagnosis and treatment of porphyrin-related diseases.
英文摘要: A high-performance liquid chromatography coupled with electrospray ionization mass spectrometry (HPLC-ESI-MS) method was developed for the simultaneous separation and detection of six porphyrins, namely uroporphyrin (UP), heptacarboxylic acid porphyrin (HEPTAP), hexacarboxylic acid porphyrin (HEXAP), pentacarboxylic acid porphyrin (PENTAP), coproporphyrin (CP), and mesoporphyrin (MP), in human urine samples. The recoveries of the porphyrins in urine ranged from 84 to 108%. The intra-and inter-day precisions at spiked porphyrin concentrations were 4.0-9.7% and 5.5-15%, respectively. The limits of detection (LOD) ranged from 0.2 to 3 nM. The developed method was successfully applied to the determination of endogenous porphyrins in human urine. UP, HEPTAP, and PENTAP were detected at levels of 42.4, 21.3, and 18.0 nM, respectively. CP was detected as the predominant species at 135.6 nM, wheres MP and HEXAP were not detected in the urine samples from healthy females. Significant increases of CP excretion in urine from liver disease patients were observed. This method proved potentially applicable for the routine diagnosis and treatment of porphyrin-related diseases.
研究领域[WOS]: Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]: CAPILLARY-ELECTROPHORESIS ; ELECTROCHEMICAL DETECTION ; FLUORESCENCE DETECTION ; HPLC METHOD ; SEPARATION ; EXTRACTION ; DIAGNOSIS ; STACKING ; ISOMERS ; CLEANUP
文章类型: Article
收录类别: SCI
原文出处: 查看原文
语种: 英语
WOS记录号: WOS:000296231000009
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.yic.ac.cn/handle/133337/5327
Appears in Collections:环境化学实验室_期刊论文

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作者单位: 1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China
2.Hong Kong Baptist Univ, Dept Chem, Hong Kong, Hong Kong, Peoples R China
3.Linyi Univ, Dept Chem, Linyi, Peoples R China
4.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China

Recommended Citation:
Li, Jinhua,Cai, Zongwei,Xu, Shoufang,et al. ANALYSIS OF URINARY PORPHYRINS BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY-ELECTROSPRAY IONIZATION MASS SPECTROMETRY[J]. JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,2011,34(15):1578-1593.
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