Metagenome of microorganisms associated with the toxic Cyanobacteria Microcystis aeruginosa analyzed using the 454 sequencing platform
Li Nan1,2,3; Zhang Lei1,2; Li Fuchao2; Wang Yuezhu4; Zhu Yongqiang4; Kang Hui4; Wang Shengyue4; Qin Song1
发表期刊CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
ISSN0254-4059
2011-05-01
卷号29期号:3页码:505-513
关键词Microcystis Aeruginosa Ectosymbiosis Diversity Cogs Algal Bloom Metagenome
产权排序[Li, N; Zhang, L; Qin, S] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China; [Li, N; Zhang, L; Li, FC] Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China; [Li, N] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China; [Wang, YZ; Zhu, YQ; Kang, H; Wang, SY] Chinese Natl Human Genome Ctr Shanghai, Shanghai MOST Key Lab Hlth & Dis Genom, Shanghai 201203, Peoples R China
通讯作者Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China. sqin@yic.ac.cn
作者部门信息集成与应用实验室 
英文摘要In this study, the 454 pyrosequencing technology was used to analyze the DNA of the Microcystis aeruginosa symbiosis system from cyanobacterial algal blooms in Taihu Lake, China. We generated 183 228 reads with an average length of 248 bp. Running the 454 assembly algorithm over our sequences yielded 22 239 significant contigs. After excluding the M. aeruginosa sequences, we obtained 1 322 assembled contigs longer than 1 000 bp. Taxonomic analysis indicated that four kingdoms were represented in the community: Archaea (n = 9; 0.01%), Bacteria (n = 98 921; 99.6%), Eukaryota (n = 373; 3.7%), and Viruses (n = 18; 0.02%). The bacterial sequences were predominantly Alphaproteobacteria (n = 41 805; 83.3%), Betaproteobacteria (n = 5 254; 10.5%) and Gammaproteobacteria (n = 1 180; 2.4%). Gene annotations and assignment of COG (clusters of orthologous groups) functional categories indicate that a large number of the predicted genes are involved in metabolic, genetic, and environmental information processes. Our results demonstrate the extraordinary diversity of a microbial community in an ectosymbiotic system and further establish the tremendous utility of pyrosequencing.; In this study, the 454 pyrosequencing technology was used to analyze the DNA of the Microcystis aeruginosa symbiosis system from cyanobacterial algal blooms in Taihu Lake, China. We generated 183 228 reads with an average length of 248 bp. Running the 454 assembly algorithm over our sequences yielded 22 239 significant contigs. After excluding the M. aeruginosa sequences, we obtained 1 322 assembled contigs longer than 1 000 bp. Taxonomic analysis indicated that four kingdoms were represented in the community: Archaea (n = 9; 0.01%), Bacteria (n = 98 921; 99.6%), Eukaryota (n = 373; 3.7%), and Viruses (n = 18; 0.02%). The bacterial sequences were predominantly Alphaproteobacteria (n = 41 805; 83.3%), Betaproteobacteria (n = 5 254; 10.5%) and Gammaproteobacteria (n = 1 180; 2.4%). Gene annotations and assignment of COG (clusters of orthologous groups) functional categories indicate that a large number of the predicted genes are involved in metabolic, genetic, and environmental information processes. Our results demonstrate the extraordinary diversity of a microbial community in an ectosymbiotic system and further establish the tremendous utility of pyrosequencing.
文章类型Article
资助机构Chinese Academy of Sciences[KSCX2-YW-G-073]
收录类别SCI
语种英语
关键词[WOS]DEGRADATION ; BACTERIA ; DIVERSITY ; BLOOMS ; COMMUNITIES ; RESERVOIR ; GENE ; SP. ; PCR ; LR
研究领域[WOS]Marine & Freshwater Biology ; Oceanography
WOS记录号WOS:000292558100002
引用统计
被引频次:34[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cn/handle/133337/5129
专题海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
作者单位1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China
2.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
4.Chinese Natl Human Genome Ctr Shanghai, Shanghai MOST Key Lab Hlth & Dis Genom, Shanghai 201203, Peoples R China
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Li Nan,Zhang Lei,Li Fuchao,et al. Metagenome of microorganisms associated with the toxic Cyanobacteria Microcystis aeruginosa analyzed using the 454 sequencing platform[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2011,29(3):505-513.
APA Li Nan.,Zhang Lei.,Li Fuchao.,Wang Yuezhu.,Zhu Yongqiang.,...&Qin Song.(2011).Metagenome of microorganisms associated with the toxic Cyanobacteria Microcystis aeruginosa analyzed using the 454 sequencing platform.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,29(3),505-513.
MLA Li Nan,et al."Metagenome of microorganisms associated with the toxic Cyanobacteria Microcystis aeruginosa analyzed using the 454 sequencing platform".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 29.3(2011):505-513.
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