| Responses of thioredoxin 1 and thioredoxin-related protein 14 mRNAs to cadmium and copper stresses in Venerupis philippinarum | |
Wang, Qing1 ; Ning, Xuanxuan2; Chen, Leilei3; Pei, Dong4; Zhao, Jianmin1; Zhang, Linbao1,5; Liu, Xiaoli1,5; Wu, Huifeng1
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| 发表期刊 | COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
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| 2011-09-01 | |
| 卷号 | 154期号:3页码:154-160 |
| 关键词 | Cadmium Copper Thioredoxin 1 Thioredoxin-related Protein 14 Venerupis Philippinarum |
| 产权排序 | [Wang, Q; Zhao, JM; Zhang, LB; Liu, XL; Wu, HF] Chinese Acad Sci, Key Lab Coastal Zone Environm Proc, CAS Shandong Prov Key Lab Coastal Zone Environm P, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China; [Ning, XX] SOA, Yantai Ocean Environm Monitoring Cent Stn, Yantai 264006, Peoples R China; [Chen, LL] Ningbo Univ, Fac Life Sci & Biotechnol, Ningbo 315211, Zhejiang, Peoples R China; [Pei, D] China Agr Univ, Yantai 264670, Peoples R China; [Zhang, LB; Liu, XL] Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China |
| 通讯作者 | Zhao, JM, Chinese Acad Sci, Key Lab Coastal Zone Environm Proc, CAS Shandong Prov Key Lab Coastal Zone Environm P, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China. jmzhao@yic.ac.cn ; hfwu@yic.ac.cn |
| 作者部门 | 污染过程与控制实验室 |
| 英文摘要 | Thioredoxin (abbreviated as Trx) is an important ubiquitous disulfide reductase, which can protect organisms against various oxidative stresses. In the present study, thioredoxin 1 (named as VpTrx1) and thioredoxin-related protein (named as VpTrp14) were identified from Venerupis philippinarum, respectively. Similar to most Trx1s, VpTrx1 possessed all conserved features critical for the fundamental structure and function of Trx1s, such as the conserved catalytic residues (C-G-P-C), but lacked the other cysteine residues, while VpTrp14 contained the conserved motif (C-P-D-C). Quantitative Real-time PCR assay showed that VpTrx1 and VpTrp14 transcripts were distributed in a wide array of tissues most abundantly expressed in the hepatopancreas. The expression of VpTrp14 mRNA in the hepatopancreas was significantly up-regulated after exposure to 10 and 40 mu g/L Cd, while the VpTrx1 expression level was kept relatively constant. Both the expression levels of VpTrx1 and VpTrp14 in the hepatopancreas were induced after exposure to Cu, and increased to the peak value at 96 h under the 40 mu g/L Cu exposure. These results showed that VpTrp14 transcripts responded to metal stress more acutely than VpTrx1, and both Trxs responded to Cu stress more sensitively than Cd. Together, it was suggested that VpTrx1 and VpTrp14 perhaps played important roles in the antioxidant responses against metal stress in V. philippinarum. (C) 2011 Elsevier Inc. All rights reserved.; Thioredoxin (abbreviated as Trx) is an important ubiquitous disulfide reductase, which can protect organisms against various oxidative stresses. In the present study, thioredoxin 1 (named as VpTrx1) and thioredoxin-related protein (named as VpTrp14) were identified from Venerupis philippinarum, respectively. Similar to most Trx1s, VpTrx1 possessed all conserved features critical for the fundamental structure and function of Trx1s, such as the conserved catalytic residues (C-G-P-C), but lacked the other cysteine residues, while VpTrp14 contained the conserved motif (C-P-D-C). Quantitative Real-time PCR assay showed that VpTrx1 and VpTrp14 transcripts were distributed in a wide array of tissues most abundantly expressed in the hepatopancreas. The expression of VpTrp14 mRNA in the hepatopancreas was significantly up-regulated after exposure to 10 and 40 mu g/L Cd, while the VpTrx1 expression level was kept relatively constant. Both the expression levels of VpTrx1 and VpTrp14 in the hepatopancreas were induced after exposure to Cu, and increased to the peak value at 96 h under the 40 mu g/L Cu exposure. These results showed that VpTrp14 transcripts responded to metal stress more acutely than VpTrx1, and both Trxs responded to Cu stress more sensitively than Cd. Together, it was suggested that VpTrx1 and VpTrp14 perhaps played important roles in the antioxidant responses against metal stress in V. philippinarum. (C) 2011 Elsevier Inc. All rights reserved. |
| 文章类型 | Article |
| 资助机构 | NSFC[30901115]; Chinese Academy of Sciences; SDSFC[ZR2009CZ008]; Key Laboratory for Ecological Environment in Coastal Areas[201011]; Key Laboratory of Marine Spill Oil Identification and Damage Assessment Technology[201115]; State Oceanic Administration |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | MARINE-INVERTEBRATES ; OXIDATIVE STRESS ; GENE-EXPRESSION ; DISK ABALONE ; BOHAI SEA ; ANTIOXIDANT ; IDENTIFICATION ; SUPERFAMILY ; APOPTOSIS ; DNA |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Endocrinology & Metabolism ; Toxicology ; Zoology |
| WOS记录号 | WOS:000293994200003 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/5123 |
| 专题 | 中国科学院海岸带环境过程与生态修复重点实验室_污染过程与控制实验室 中国科学院海岸带环境过程与生态修复重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Coastal Zone Environm Proc, CAS Shandong Prov Key Lab Coastal Zone Environm P, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China 2.SOA, Yantai Ocean Environm Monitoring Cent Stn, Yantai 264006, Peoples R China 3.Ningbo Univ, Fac Life Sci & Biotechnol, Ningbo 315211, Zhejiang, Peoples R China 4.China Agr Univ, Yantai 264670, Peoples R China 5.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Qing,Ning, Xuanxuan,Chen, Leilei,et al. Responses of thioredoxin 1 and thioredoxin-related protein 14 mRNAs to cadmium and copper stresses in Venerupis philippinarum[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY,2011,154(3):154-160. |
| APA | Wang, Qing.,Ning, Xuanxuan.,Chen, Leilei.,Pei, Dong.,Zhao, Jianmin.,...&Wu, Huifeng.(2011).Responses of thioredoxin 1 and thioredoxin-related protein 14 mRNAs to cadmium and copper stresses in Venerupis philippinarum.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY,154(3),154-160. |
| MLA | Wang, Qing,et al."Responses of thioredoxin 1 and thioredoxin-related protein 14 mRNAs to cadmium and copper stresses in Venerupis philippinarum".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY 154.3(2011):154-160. |
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