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Metabolomic Study on the Halophyte Suaeda salsa in the Yellow River Delta
Liu, Xiaoli1,2; Zhang, Linbao1,2; You, Liping1,2; Wu, Huifeng1; Zhao, Jianmin1,2; Cong, Ming1,2; Li, Fei1,2; Wang, Qing1,2; Li, Lianzhen1,2; Li, Chenghua1,2; Han, Guangxuan1,2; Wang, Guangmei1,2; Xia, Chuanhai1,2; Yu, Junbao1,2
2011-08-01
发表期刊CLEAN-SOIL AIR WATER
ISSN1863-0650
卷号39期号:8页码:720-727
产权排序[Liu, XL; Zhang, LB; You, LP; Wu, HF; Zhao, JM; Cong, M; Li, F; Wang, Q; Li, LZ; Li, CH; Han, GX; Wang, GM; Xia, CH; Yu, JB] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Shandong Prov Key Lab Coastal Zone Environm Proc, Key Lab Coastal Zone Environm Proc,CAS, Yantai 264003, Peoples R China; [Liu, XL; Zhang, LB; You, LP; Zhao, JM; Cong, M; Li, F; Wang, Q; Li, LZ; Li, CH; Han, GX; Wang, GM; Xia, CH; Yu, JB] Chinese Acad Sci, Grad Sch, Beijing, Peoples R China
摘要Plant metabolomics has been well established and applied across multiple fields including medicine, biotechnology, and environmental sciences in the post-genomic era. The Chenopodiaceae C(3) halophyte Suaeda salsa is the most important plant species in the vegetation of saline soil and even intertidal zone in the Yellow River Delta, which is economically consumed as food, widely used as a bioindicator of environmental stresses (salinity, drought, and pollution) and typically applied for the phyto-remediation of degraded wetland. However, no global studies have been focused on the metabolic profile of this halophyte which is widely applied in environment related research areas. In metabolomics, the first crucial step is the preparation of plant samples. In this work, several strategies of metabolite extraction from this C(3) halophytes S. salsa were evaluated and the metabolic profile was characterized by NMRbased metabolomics. Multiple replicates of plant tissues (approx. 250 mg whole fresh weight of leaves and stems) were homogenized by a high throughput automated Precellys 24 bead-based homogenizer and then extracted using the following solvent systems of varying polarities:methanol, water, methanol/water, methanol/chloroform/water, and acetonitrile. The hydrophilic metabolites were analyzed using one-dimensional proton NMR spectroscopy, and the subsequent NMR spectra were evaluated by principal components analysis (PCA). Each extraction protocol revealed unique metabolic profile from S. salsa. Overall, the quality of each extraction protocol was assessed based on the yield, reproducibility, ease, and speed, and we concluded that the solvent of methanol/water was preferable for the metabolite extraction due to its highest reproducibility, ease, and speed, and considerably high yield. Validation of methanol/ water (1:1) solvent also showed a high degree of reproducibility with technical variation being considerably lower than biological variation in S. salsa samples. This demonstrated the quality and robustness of the methanol/water extraction method for NMRbased metabolomics studies.; Plant metabolomics has been well established and applied across multiple fields including medicine, biotechnology, and environmental sciences in the post-genomic era. The Chenopodiaceae C-3 halophyte Suaeda salsa is the most important plant species in the vegetation of saline soil and even intertidal zone in the Yellow River Delta, which is economically consumed as food, widely used as a bioindicator of environmental stresses (salinity, drought, and pollution) and typically applied for the phyto-remediation of degraded wetland. However, no global studies have been focused on the metabolic profile of this halophyte which is widely applied in environment related research areas. In metabolomics, the first crucial step is the preparation of plant samples. In this work, several strategies of metabolite extraction from this C-3 halophytes S. salsa were evaluated and the metabolic profile was characterized by NMRbased metabolomics. Multiple replicates of plant tissues (approx. 250 mg whole fresh weight of leaves and stems) were homogenized by a high throughput automated Precellys 24 bead-based homogenizer and then extracted using the following solvent systems of varying polarities:methanol, water, methanol/water, methanol/chloroform/water, and acetonitrile. The hydrophilic metabolites were analyzed using one-dimensional proton NMR spectroscopy, and the subsequent NMR spectra were evaluated by principal components analysis (PCA). Each extraction protocol revealed unique metabolic profile from S. salsa. Overall, the quality of each extraction protocol was assessed based on the yield, reproducibility, ease, and speed, and we concluded that the solvent of methanol/water was preferable for the metabolite extraction due to its highest reproducibility, ease, and speed, and considerably high yield. Validation of methanol/ water (1:1) solvent also showed a high degree of reproducibility with technical variation being considerably lower than biological variation in S. salsa samples. This demonstrated the quality and robustness of the methanol/water extraction method for NMRbased metabolomics studies.
通讯作者Wu, HF, Chinese Acad Sci, Yantai Inst Coastal Zone Res, Shandong Prov Key Lab Coastal Zone Environm Proc, Key Lab Coastal Zone Environm Proc,CAS, Yantai 264003, Peoples R China. hfwu@yic.ac.cn
关键词Metabolite Extraction Metabolomics Nmr Suaeda Salsa
作者部门污染过程与控制实验室 
学科领域Environmental Sciences & Ecology ; Marine & Freshwater Biology ; Water Resources
项目资助者National Science & Technology Pillar Program in "12th Five Year'' Period[2011BAC02B01]; Chinese Academy of Sciences[KZCX2-YW-223, KZCX2-YW-225]; Technology Development Program Projects of Shandong Province[2008GG20005006, 2008GG3NS0700]; SDSFC[ZR2009CZ008]; CAS/SAFEA; Shandong Provincial Key Laboratory of Ecology and Environment in the Yellow River Delta[2009KFJJ03]
收录类别SCI
关键词[WOS]V-H+-ATPASE ; BETAINE-HOMOCYSTEINE METHYLTRANSFERASE ; PLANT METABOLOMICS ; SAMPLE-PREPARATION ; NMR-SPECTROSCOPY ; OSMOTIC-STRESS ; LEAVES ; EXTRACTION ; LEAF ; ACCUMULATION
文章类型Article
语种英语
WOS研究方向Environmental Sciences & Ecology ; Marine & Freshwater Biology ; Water Resources
WOS记录号WOS:000294720700002
引用统计
被引频次:11[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cn/handle/133337/5122
专题污染过程与控制实验室
中科院海岸带环境过程与生态修复重点实验室
山东省海岸带环境过程重点实验室
作者单位1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Shandong Prov Key Lab Coastal Zone Environm Proc, Key Lab Coastal Zone Environm Proc,CAS, Yantai 264003, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China
第一作者单位中国科学院烟台海岸带研究所
推荐引用方式
GB/T 7714
Liu, Xiaoli,Zhang, Linbao,You, Liping,et al. Metabolomic Study on the Halophyte Suaeda salsa in the Yellow River Delta[J]. CLEAN-SOIL AIR WATER,2011,39(8):720-727.
APA Liu, Xiaoli.,Zhang, Linbao.,You, Liping.,Wu, Huifeng.,Zhao, Jianmin.,...&Yu, Junbao.(2011).Metabolomic Study on the Halophyte Suaeda salsa in the Yellow River Delta.CLEAN-SOIL AIR WATER,39(8),720-727.
MLA Liu, Xiaoli,et al."Metabolomic Study on the Halophyte Suaeda salsa in the Yellow River Delta".CLEAN-SOIL AIR WATER 39.8(2011):720-727.
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