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题名:
Metabolomic Study on the Halophyte Suaeda salsa in the Yellow River Delta
作者: Liu, Xiaoli1,2; Zhang, Linbao1,2; You, Liping1,2; Wu, HuiFeng(吴惠丰)1; Zhao, JianMin(赵建民)1,2; Cong, Ming1,2; Li, Fei(李斐)1,2; Wang, Qing(王清)1,2; Li, Lianzhen1,2; Li, Chenghua1,2; Han Guangxuan(韩广轩)1,2; Wang, GuangMei(王光美)1,2; Xia, ChuanHai(夏传海)1,2; Yu, Junbao1,2
刊名: CLEAN-SOIL AIR WATER
ISSN号: 1863-0650
出版日期: 2011-08-01
卷号: 39, 期号:8, 页码:720-727
关键词: Metabolite extraction ; Metabolomics ; NMR ; Suaeda salsa
学科分类: Environmental Sciences & Ecology; Marine & Freshwater Biology; Water Resources
产权排序: [Liu, XL; Zhang, LB; You, LP; Wu, HF; Zhao, JM; Cong, M; Li, F; Wang, Q; Li, LZ; Li, CH; Han, GX; Wang, GM; Xia, CH; Yu, JB] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Shandong Prov Key Lab Coastal Zone Environm Proc, Key Lab Coastal Zone Environm Proc,CAS, Yantai 264003, Peoples R China; [Liu, XL; Zhang, LB; You, LP; Zhao, JM; Cong, M; Li, F; Wang, Q; Li, LZ; Li, CH; Han, GX; Wang, GM; Xia, CH; Yu, JB] Chinese Acad Sci, Grad Sch, Beijing, Peoples R China
通讯作者: Wu, HF, Chinese Acad Sci, Yantai Inst Coastal Zone Res, Shandong Prov Key Lab Coastal Zone Environm Proc, Key Lab Coastal Zone Environm Proc,CAS, Yantai 264003, Peoples R China. hfwu@yic.ac.cn
作者部门: 污染过程与控制实验室 
中文摘要: Plant metabolomics has been well established and applied across multiple fields including medicine, biotechnology, and environmental sciences in the post-genomic era. The Chenopodiaceae C(3) halophyte Suaeda salsa is the most important plant species in the vegetation of saline soil and even intertidal zone in the Yellow River Delta, which is economically consumed as food, widely used as a bioindicator of environmental stresses (salinity, drought, and pollution) and typically applied for the phyto-remediation of degraded wetland. However, no global studies have been focused on the metabolic profile of this halophyte which is widely applied in environment related research areas. In metabolomics, the first crucial step is the preparation of plant samples. In this work, several strategies of metabolite extraction from this C(3) halophytes S. salsa were evaluated and the metabolic profile was characterized by NMRbased metabolomics. Multiple replicates of plant tissues (approx. 250 mg whole fresh weight of leaves and stems) were homogenized by a high throughput automated Precellys 24 bead-based homogenizer and then extracted using the following solvent systems of varying polarities:methanol, water, methanol/water, methanol/chloroform/water, and acetonitrile. The hydrophilic metabolites were analyzed using one-dimensional proton NMR spectroscopy, and the subsequent NMR spectra were evaluated by principal components analysis (PCA). Each extraction protocol revealed unique metabolic profile from S. salsa. Overall, the quality of each extraction protocol was assessed based on the yield, reproducibility, ease, and speed, and we concluded that the solvent of methanol/water was preferable for the metabolite extraction due to its highest reproducibility, ease, and speed, and considerably high yield. Validation of methanol/ water (1:1) solvent also showed a high degree of reproducibility with technical variation being considerably lower than biological variation in S. salsa samples. This demonstrated the quality and robustness of the methanol/water extraction method for NMRbased metabolomics studies.
英文摘要: Plant metabolomics has been well established and applied across multiple fields including medicine, biotechnology, and environmental sciences in the post-genomic era. The Chenopodiaceae C-3 halophyte Suaeda salsa is the most important plant species in the vegetation of saline soil and even intertidal zone in the Yellow River Delta, which is economically consumed as food, widely used as a bioindicator of environmental stresses (salinity, drought, and pollution) and typically applied for the phyto-remediation of degraded wetland. However, no global studies have been focused on the metabolic profile of this halophyte which is widely applied in environment related research areas. In metabolomics, the first crucial step is the preparation of plant samples. In this work, several strategies of metabolite extraction from this C-3 halophytes S. salsa were evaluated and the metabolic profile was characterized by NMRbased metabolomics. Multiple replicates of plant tissues (approx. 250 mg whole fresh weight of leaves and stems) were homogenized by a high throughput automated Precellys 24 bead-based homogenizer and then extracted using the following solvent systems of varying polarities:methanol, water, methanol/water, methanol/chloroform/water, and acetonitrile. The hydrophilic metabolites were analyzed using one-dimensional proton NMR spectroscopy, and the subsequent NMR spectra were evaluated by principal components analysis (PCA). Each extraction protocol revealed unique metabolic profile from S. salsa. Overall, the quality of each extraction protocol was assessed based on the yield, reproducibility, ease, and speed, and we concluded that the solvent of methanol/water was preferable for the metabolite extraction due to its highest reproducibility, ease, and speed, and considerably high yield. Validation of methanol/ water (1:1) solvent also showed a high degree of reproducibility with technical variation being considerably lower than biological variation in S. salsa samples. This demonstrated the quality and robustness of the methanol/water extraction method for NMRbased metabolomics studies.
研究领域[WOS]: Environmental Sciences & Ecology ; Marine & Freshwater Biology ; Water Resources
关键词[WOS]: V-H+-ATPASE ; BETAINE-HOMOCYSTEINE METHYLTRANSFERASE ; PLANT METABOLOMICS ; SAMPLE-PREPARATION ; NMR-SPECTROSCOPY ; OSMOTIC-STRESS ; LEAVES ; EXTRACTION ; LEAF ; ACCUMULATION
文章类型: Article
收录类别: SCI
语种: 英语
WOS记录号: WOS:000294720700002
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.yic.ac.cn/handle/133337/5122
Appears in Collections:中科院海岸带环境过程与生态修复重点实验室_期刊论文
污染过程与控制实验室_期刊论文
山东省海岸带环境过程重点实验室_期刊论文

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作者单位: 1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Shandong Prov Key Lab Coastal Zone Environm Proc, Key Lab Coastal Zone Environm Proc,CAS, Yantai 264003, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China

Recommended Citation:
Liu, Xiaoli,Zhang, Linbao,You, Liping,et al. Metabolomic Study on the Halophyte Suaeda salsa in the Yellow River Delta[J]. CLEAN-SOIL AIR WATER,2011,39(8):720-727.
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