Polymeric Membrane Ion-Selective Electrode for Butyrylcholinesterase Based on Controlled Release of Substrate

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	Polymeric Membrane Ion-Selective Electrode for Butyrylcholinesterase Based on Controlled Release of Substrate
	Ding, Jiawang1,2,3 ; Qin, Wei1,4
发表期刊	ELECTROANALYSIS
	2009-09-01
卷号	21 期号:17-18 页码:2030-2035
关键词	Polymer Membrane Electrodes Controlled-release Butyrylcholinesterase Pesticides Biosensors Membranes
产权排序	[Ding, Jiawang; Qin, Wei] Chinese Acad Sci, Environm Chem Lab, Yantai Inst Coastal Zone Res Sustainable Dev, Yantai 264003, Peoples R China; [Ding, Jiawang] Chinese Acad Sci, S China Sea Inst Oceanol, Guangzhou 510301, Guangdong, Peoples R China; [Ding, Jiawang] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China; [Qin, Wei] Yantai Univ, Sch Oceanol, Yantai 264005, Peoples R China
通讯作者	Qin, W, Chinese Acad Sci, Environm Chem Lab, Yantai Inst Coastal Zone Res Sustainable Dev, Yantai 264003, Peoples R China
作者部门	环境化学实验室
英文摘要	A butyrylcholine selective polymeric membrane electrode using o-beta-cyclodextrin as ionophore is described as a reagent controlled-release system for reagentless detection of butyrylcholinesterase. Butyrylcholine released across the membrane from inner filling solution of the ion selective electrode is consumed by reaction of the enzyme in sample solution and the concentration of butyrylcholine at the membrane surface can be sensed potentiometrically. The electrode with 0.01 M butyrylcholine in the inner solution yields a potential that varies linearly with butyrylcholinesterase concentration over the range of 0.0075-0.15 U mL(-1) in 0.02 M of pH 7.4 sodium phosphate buffer solution. This approach can also be used to analyze butyrylcholinesterase inhibitors such as organophosphate pesticides. The inhibition percentage of parathion is proportional to its concentration in the range of 0.05-0.5 ng mL(-1) with a detection limit of 0.03 ng mL(-1).; A butyrylcholine selective polymeric membrane electrode using o-beta-cyclodextrin as ionophore is described as a reagent controlled-release system for reagentless detection of butyrylcholinesterase. Butyrylcholine released across the membrane from inner filling solution of the ion selective electrode is consumed by reaction of the enzyme in sample solution and the concentration of butyrylcholine at the membrane surface can be sensed potentiometrically. The electrode with 0.01 M butyrylcholine in the inner solution yields a potential that varies linearly with butyrylcholinesterase concentration over the range of 0.0075-0.15 U mL(-1) in 0.02 M of pH 7.4 sodium phosphate buffer solution. This approach can also be used to analyze butyrylcholinesterase inhibitors such as organophosphate pesticides. The inhibition percentage of parathion is proportional to its concentration in the range of 0.05-0.5 ng mL(-1) with a detection limit of 0.03 ng mL(-1).
文章类型	Article
收录类别	SCI ; ISTP
语种	英语
关键词[WOS]	LOWER DETECTION LIMIT ; POTENTIOMETRIC SENSORS ; ELECTROCHEMICAL ASSAY ; AU NANOPARTICLES ; FLUXES ; ACETYLCHOLINESTERASE ; INHIBITION ; TITRATIONS ; MECHANISM ; CHITOSAN
研究领域[WOS]	Chemistry ; Electrochemistry
WOS记录号	WOS:000270399100020
引用统计	被引频次：17[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/4938
专题	中国科学院海岸带环境过程与生态修复重点实验室_海岸带环境过程实验室
作者单位	1.Chinese Acad Sci, Environm Chem Lab, Yantai Inst Coastal Zone Res Sustainable Dev, Yantai 264003, Peoples R China 2.Chinese Acad Sci, S China Sea Inst Oceanol, Guangzhou 510301, Guangdong, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 4.Yantai Univ, Sch Oceanol, Yantai 264005, Peoples R China
推荐引用方式 GB/T 7714	Ding, Jiawang,Qin, Wei. Polymeric Membrane Ion-Selective Electrode for Butyrylcholinesterase Based on Controlled Release of Substrate[J]. ELECTROANALYSIS,2009,21(17-18):2030-2035.
APA	Ding, Jiawang,&Qin, Wei.(2009).Polymeric Membrane Ion-Selective Electrode for Butyrylcholinesterase Based on Controlled Release of Substrate.ELECTROANALYSIS,21(17-18),2030-2035.
MLA	Ding, Jiawang,et al."Polymeric Membrane Ion-Selective Electrode for Butyrylcholinesterase Based on Controlled Release of Substrate".ELECTROANALYSIS 21.17-18(2009):2030-2035.

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