Method of mRNA display for selecting peptides that bind with target enzyme RNA
Yan,Song ; Wang,Zheng ; Yang,Shao-Li ; Zhang,Yi ; Lin,Xiu-Kun
通讯作者Yan, S. (yansong209@yahoo.com.cn)
会议主办者IEEE Engineering in Medicine and Biology Society; Wuhan University; Fuzhou University; Nankai University; Overs. Chin. Sch. Environ. Prot. Assoc. (OCSEPA) -
2011
会议名称5th International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2011
会议录名称8th International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2011
会议日期2011-05-13
会议地点Wuhan, China
产权排序(1)Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071, China; (2)School of Environmental Science and Engineering, Dalian Jiaotong University, Dalian 116028, China; (3)Yantai Institute of Coastal Zone Research for Sustainable Development, Chinese Academy of Sciences, Yantai, 264003, China
摘要In vitro display techniques are useful tools for selecting novel ligands that bind to target biomolecules. In this paper, mRNA display method was used to select peptides that bind thymidylate synthase RNA with high affinity from a peptide library. We provide a detailed analysis of the critical steps of an mRNA-display-based selection, including the generation of an mRNA-displayed library, formation of RNA-peptide fusions, and selection of RNA affinity peptides. After optimization of the experimental steps, the efficiency of RNA-linker purification and the reverse transcription was improved, and the unspecified binding peptides were removed. Our study could facilitate the use of mRNA display in the field of biotechnology, medicine and proteomics. © 2011 IEEE.
作者部门生物资源实验室
关键词Peptides Bioinformatics Medicine Molecular Biology Rna
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收录类别EI
文献类型会议论文
条目标识符http://ir.yic.ac.cn/handle/133337/4935
专题海岸带生物学与生物资源利用所重点实验室
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GB/T 7714
Yan,Song,Wang,Zheng,Yang,Shao-Li,et al. Method of mRNA display for selecting peptides that bind with target enzyme RNA[C],2011.
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