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题名:
Method of mRNA display for selecting peptides that bind with target enzyme RNA
作者: Yan,Song ; Wang,Zheng ; Yang,Shao-Li ; Zhang,Yi ; Lin,Xiu-Kun
通讯作者: Yan, S. (yansong209@yahoo.com.cn)
产权排序: (1)Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071, China; (2)School of Environmental Science and Engineering, Dalian Jiaotong University, Dalian 116028, China; (3)Yantai Institute of Coastal Zone Research for Sustainable Development, Chinese Academy of Sciences, Yantai, 264003, China
会议名称: 5th International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2011
会议主办者: IEEE Engineering in Medicine and Biology Society; Wuhan University; Fuzhou University; Nankai University; Overs. Chin. Sch. Environ. Prot. Assoc. (OCSEPA) -
会议日期: 2011-05-13
会议地点: Wuhan, China
会议录: 8th International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2011
出版日期: 2011
关键词: Peptides ; Bioinformatics ; Medicine ; Molecular biology ; RNA
中文摘要: In vitro display techniques are useful tools for selecting novel ligands that bind to target biomolecules. In this paper, mRNA display method was used to select peptides that bind thymidylate synthase RNA with high affinity from a peptide library. We provide a detailed analysis of the critical steps of an mRNA-display-based selection, including the generation of an mRNA-displayed library, formation of RNA-peptide fusions, and selection of RNA affinity peptides. After optimization of the experimental steps, the efficiency of RNA-linker purification and the reverse transcription was improved, and the unspecified binding peptides were removed. Our study could facilitate the use of mRNA display in the field of biotechnology, medicine and proteomics. © 2011 IEEE.
作者部门: 生物资源实验室
收录类别: EI
内容类型: 会议论文
URI标识: http://ir.yic.ac.cn/handle/133337/4935
Appears in Collections:海岸带生物学与生物资源利用所重点实验室_会议论文

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Recommended Citation:
Yan,Song,Wang,Zheng,Yang,Shao-Li,et al. Method of mRNA display for selecting peptides that bind with target enzyme RNA[C]. 见:5th International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2011. Wuhan, China. 2011-05-13.http://dx.doi.org/10.1109/icbbe.2011.5780178.
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