Allelochemical ethyl 2-methyl acetoacetate (EMA) induces oxidative damage and antioxidant responses in Phaeodactylum tricornutum
Yang, Cui-Yun; Liu, Su-Jing; Zhou, Shi-Wei; Wu, Hui-Feng; Yu, Jun-Bao; Xia, Chuan-Hai
发表期刊PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
ISSN0048-3575
2011-05-01
卷号100期号:1页码:93-103
关键词Phaeodactylum Tricornutum Ethyl 2-mEthyl Acetoacetate Reactive Oxygen Species Malondialdehyde Antioxidant Enzyme
产权排序[Yang, Cui-Yun; Liu, Su-Jing; Zhou, Shi-Wei; Wu, Hui-Feng; Yu, Jun-Bao; Xia, Chuan-Hai] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China
通讯作者Xia, CH, Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China.chxia@yic.ac.cn
作者部门生物资源实验室 
英文摘要Ethyl 2-methyl acetoacetate (EMA) is a novel allelochemical exhibiting inhibitory effects on the growth of marine unicellular alga Phaeodactylum tricornutum (P. tricornutum). Oxidative damage and antioxidant responses in P. tricornutum were investigated to elucidate the mechanism involved in EMA inhibition on algal growth. The increase in reactive oxygen species (ROS) levels and malondialdehyde (MDA) contents following exposure to EMA suggested that alga was suffered from oxidative stress and severely damaged. The decrease in cell activity and cellular inclusions suggested that cell growth was greatly inhibited. The activities of the antioxidant enzymes including superoxide dismutase (SOD), catalase (CAT), glutathione reductase (GR), glutathione peroxide (GSH-PX) and glutathione S-transferase (GST) increased with the exposure concentration and decreased with the prolongation of exposure time. Cellular ascorbic acid (AsA) and reduced glutathione (GSH) systems were also involved in resisting oxidative stress of EMA by altering the composition of AsA and GSH pools. EMA exposure increased the contents of AsA, GSH, dehydroascorbate (DAsA) and glutathione (GSSG). However, the regeneration rate of AsA/DAsA did not change obviously between treatments and the control, while that of GSH/GSSG decreased significantly under 14 mmol/L EMA exposure on the 3rd day. These results showed that EMA-induced oxidative damage might be responsible for EMA inhibition on P. tricornutum growth and cellular antioxidant enzymes and non-enzymatic antioxidants were improved to counteract the oxidative stress. (C) 2011 Elsevier Inc. All rights reserved.
文章类型Article
资助机构National Natural Science Foundation of China [21007088]; Research Grant for Outstanding Young Scientists of Shandong Province [BS2009CL006]; National Key Technology R&D Program of China [2008BAC32B03]; Yantai Science and Technology Bureau [2008155]
收录类别SCI
语种英语
关键词[WOS]CYANOBACTERIUM MICROCYSTIS-AERUGINOSA ; ALGA SELENASTRUM-CAPRICORNUTUM ; ASCORBATE-GLUTATHIONE CYCLE ; LIPID-PEROXIDATION ; SUPEROXIDE-DISMUTASE ; MYRIOPHYLLUM-SPICATUM ; SIGNAL-TRANSDUCTION ; PHRAGMITES-COMMUNIS ; ENZYME-ACTIVITIES ; CUCUMBER LEAVES
研究领域[WOS]Biochemistry & Molecular Biology ; Entomology ; Physiology
WOS记录号WOS:000289499900015
引用统计
被引频次:70[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cn/handle/133337/4867
专题海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
中国科学院海岸带环境过程与生态修复重点实验室_滨海湿地实验室
作者单位Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China
推荐引用方式
GB/T 7714
Yang, Cui-Yun,Liu, Su-Jing,Zhou, Shi-Wei,et al. Allelochemical ethyl 2-methyl acetoacetate (EMA) induces oxidative damage and antioxidant responses in Phaeodactylum tricornutum[J]. PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY,2011,100(1):93-103.
APA Yang, Cui-Yun,Liu, Su-Jing,Zhou, Shi-Wei,Wu, Hui-Feng,Yu, Jun-Bao,&Xia, Chuan-Hai.(2011).Allelochemical ethyl 2-methyl acetoacetate (EMA) induces oxidative damage and antioxidant responses in Phaeodactylum tricornutum.PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY,100(1),93-103.
MLA Yang, Cui-Yun,et al."Allelochemical ethyl 2-methyl acetoacetate (EMA) induces oxidative damage and antioxidant responses in Phaeodactylum tricornutum".PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY 100.1(2011):93-103.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
Allelochemical ethyl(354KB)期刊论文出版稿开放获取CC BY-NC-SA浏览 请求全文
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Yang, Cui-Yun]的文章
[Liu, Su-Jing]的文章
[Zhou, Shi-Wei]的文章
百度学术
百度学术中相似的文章
[Yang, Cui-Yun]的文章
[Liu, Su-Jing]的文章
[Zhou, Shi-Wei]的文章
必应学术
必应学术中相似的文章
[Yang, Cui-Yun]的文章
[Liu, Su-Jing]的文章
[Zhou, Shi-Wei]的文章
相关权益政策
暂无数据
收藏/分享
文件名: Allelochemical ethyl 2-methyl acetoacetate (EMA) induces oxidative damage and antioxidant responses in Phaeodactylum tricornutum.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。