| Serine/Threonine Protein Kinase SpkG Is a Candidate for High Salt Resistance in the Unicellular Cyanobacterium Synechocystis sp PCC 6803 | |
Liang, Chengwei1,2; Zhang, Xiaowen2,3; Chi, Xiaoyuan2; Guan, Xiangyu4; Li, Youxun2; Qin, Song2 ; Shao, Hong Bo5
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| 发表期刊 | PLOS ONE
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| ISSN | 1932-6203 |
| 2011-05-26 | |
| 卷号 | 6期号:5页码:e18718 |
| 关键词 | Sp Pcc-6803 Signal-transduction Genes Stress Expression Bacteria Competence Perception Systems Xanthus |
| 产权排序 | [Liang, Chengwei] Qingdao Univ Sci & Technol, Qingdao, Peoples R China; [Liang, Chengwei; Zhang, Xiaowen; Chi, Xiaoyuan; Li, Youxun; Qin, Song] Chinese Acad Sci, Inst Oceanol, Qingdao, Peoples R China; [Zhang, Xiaowen] Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao, Peoples R China; [Guan, Xiangyu] Ocean Univ China, Qingdao, Peoples R China; [Shao, Hong Bo] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai, Peoples R China |
| 通讯作者 | Liang, CW, Qingdao Univ Sci & Technol, Qingdao, Peoples R China.sqin@yic.ac.cn |
| 作者部门 | 滨海湿地实验室 |
| 英文摘要 | Background: Seven serine/threonine kinase genes have been predicted in unicellular cyanobacterium Synechocystis sp. PCC6803. SpkA and SpkB were shown to be required for cell motility and SpkE has no kinase activity. There is no report whether the other four STKs are involved in stress-mediated signaling in Synechocystis PCC6803. Methodology/Principal Findings: In this paper, we examined differential expression of the other four serine/threonine kinases, SpkC, SpkD, SpkF and SpkG, at seven different stress conditions. The transcriptional level was up-regulated of spkG and down-regulated of spkC under high salt stress condition. Two spk deletion mutants, Delta spkC and Delta spkG, were constructed and their growth characteristic were examined compared to the wild strain. The wild strain and Delta spkC mutant were not affected under high salt stress conditions. In contrast, growth of spkG mutant was completely impaired. To further confirm the function of spkG, we also examined the effect of mutation of spkG on the expression of salt stress-inducible genes. We compared genome-wide patterns of transcription between wild-type Synechocystis sp. PCC6803 and cells with a mutation in the SpkG with DNA microarray analysis. Conclusion: In this study, we first study the spkG gene as sensor of high salt signal. We consider that SpkG play essential roles in Synechocystis sp. for sensing the high salt signal directly, rather than mediating signals among other kinases. Our microarray experiment may help select relatively significant genes for further research on mechanisms of signal transduction of Synechocystis sp. PCC6803 under high salt stress.; Background: Seven serine/threonine kinase genes have been predicted in unicellular cyanobacterium Synechocystis sp. PCC6803. SpkA and SpkB were shown to be required for cell motility and SpkE has no kinase activity. There is no report whether the other four STKs are involved in stress-mediated signaling in Synechocystis PCC6803. |
| 文章类型 | Article |
| 资助机构 | Chinese Academy of Sciences [KZCX2-YW-223]; National Natural Science Foundation of China [40803023]; Key Program of Natural Science Foundation of Shandong Province [ZR2010DZ001]; Talents Foundation of Chinese Academy of Sciences [AJ0809BX-036] |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | SP PCC-6803 ; SIGNAL-TRANSDUCTION ; GENES ; STRESS ; EXPRESSION ; BACTERIA ; COMPETENCE ; PERCEPTION ; SYSTEMS ; XANTHUS |
| 研究领域[WOS] | Science & Technology - Other Topics |
| WOS记录号 | WOS:000291052200008 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/4829 |
| 专题 | 中国科学院海岸带环境过程与生态修复重点实验室_滨海湿地实验室 海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室 |
| 作者单位 | 1.Qingdao Univ Sci & Technol, Qingdao, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Qingdao, Peoples R China 3.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao, Peoples R China 4.Ocean Univ China, Qingdao, Peoples R China 5.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liang, Chengwei,Zhang, Xiaowen,Chi, Xiaoyuan,et al. Serine/Threonine Protein Kinase SpkG Is a Candidate for High Salt Resistance in the Unicellular Cyanobacterium Synechocystis sp PCC 6803[J]. PLOS ONE,2011,6(5):e18718. |
| APA | Liang, Chengwei.,Zhang, Xiaowen.,Chi, Xiaoyuan.,Guan, Xiangyu.,Li, Youxun.,...&Shao, Hong Bo.(2011).Serine/Threonine Protein Kinase SpkG Is a Candidate for High Salt Resistance in the Unicellular Cyanobacterium Synechocystis sp PCC 6803.PLOS ONE,6(5),e18718. |
| MLA | Liang, Chengwei,et al."Serine/Threonine Protein Kinase SpkG Is a Candidate for High Salt Resistance in the Unicellular Cyanobacterium Synechocystis sp PCC 6803".PLOS ONE 6.5(2011):e18718. |
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