Identification of two small heat shock proteins with different response profile to cadmium and pathogen stresses in Venerupis philippinarum
Li, Chenghua1; Wang, Lingling2; Ning, Xuanxuan3; Chen, Aiqin1; Zhang, Linbao1; Qin, Song1; Wu, Huifeng1; Zhao, Jianmin1
2010-11-01
Source PublicationCELL STRESS & CHAPERONES
ISSN1355-8145
Volume15Issue:6Pages:897-904
Contribution Rank[Li, Chenghua; Chen, Aiqin; Zhang, Linbao; Qin, Song; Wu, Huifeng; Zhao, Jianmin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China; [Wang, Lingling] Chinese Acad Sci, Key Lab Expt Marine Biol, Inst Oceanol, Qingdao 266071, Peoples R China; [Ning, Xuanxuan] SOA, Yantai Ocean Environm Monitoring Cent Stn, Yantai 264006, Peoples R China
AbstractSmall heat shock proteins (sHSPs) encompass a widespread and diverse class of proteins with molecular chaperone activity. In the present study, two sHSP isoforms (VpsHSP-1 and VpsHSP-2) were cloned from Venerupis philippinarum haemocytes by Rapid Amplification of cDNA Ends (RACE) approaches. The expression profiles of these two genes under Vibrio anguillarum challenge and cadmium exposure were investigated by quantitative real-time reverse transcriptase polymerase chain reaction. The bacterial challenge could significantly up-regulate the mRNA expression of both VpsHSP-1 and VpsHSP-2, with the increase of VpsHSP-2 expression occurred earlier than that of VpsHSP-1. During the cadmium exposure experiment, the expression level of both VpsHSP-1 and VpsHSP-2 decreased significantly with larger amplitude in VpsHSP-2. As time progressed, the expression levels of both genes were up-regulated with more increment in the low-chemical exposure groups. The differences in the response to pathogen stimulation and cadmium exposure indicated that there were functional diversity between the two structurally different molecules, VpsHSP-1 and VpsHSP-2, and they probably played distinct roles in mediating the environmental stress and immune responses in calm.; Small heat shock proteins (sHSPs) encompass a widespread and diverse class of proteins with molecular chaperone activity. In the present study, two sHSP isoforms (VpsHSP-1 and VpsHSP-2) were cloned from Venerupis philippinarum haemocytes by Rapid Amplification of cDNA Ends (RACE) approaches. The expression profiles of these two genes under Vibrio anguillarum challenge and cadmium exposure were investigated by quantitative real-time reverse transcriptase polymerase chain reaction. The bacterial challenge could significantly up-regulate the mRNA expression of both VpsHSP-1 and VpsHSP-2, with the increase of VpsHSP-2 expression occurred earlier than that of VpsHSP-1. During the cadmium exposure experiment, the expression level of both VpsHSP-1 and VpsHSP-2 decreased significantly with larger amplitude in VpsHSP-2. As time progressed, the expression levels of both genes were up-regulated with more increment in the low-chemical exposure groups. The differences in the response to pathogen stimulation and cadmium exposure indicated that there were functional diversity between the two structurally different molecules, VpsHSP-1 and VpsHSP-2, and they probably played distinct roles in mediating the environmental stress and immune responses in calm.
Corresponding AuthorZhao, JM, Chinese Acad Sci, Yantai Inst Coastal Zone Res, 17 Chunhui Rd, Yantai 264003, Peoples R China
KeywordVenerupis Philippinarum Small Heat Shock Protein Time-course Expression Vibrio Anguillarum Cadmium Exposure
Department污染过程与控制实验室
Subject AreaCell Biology
Funding OrganizationKey Laboratory of Experimental Marine Biology (Institute of Oceanology, CAS) ; Chinese Academy of Sciences [kzcx2-yw-225, KZCX2-YW-Q07-04]; NSFC [30901115]; SDSFC [ZR2009CZ008]
Indexed BySCI
WOS KeywordIMMUNE-RESPONSE ; EXPRESSION ; GENE ; EVOLUTIONARY ; REGULATORS ; INNATE ; ROLES
SubtypeArticle
Language英语
WOS Research AreaCell Biology
WOS IDWOS:000282971000013
Citation statistics
Document Type期刊论文
Identifierhttp://ir.yic.ac.cn/handle/133337/4415
Collection中科院海岸带环境过程与生态修复重点实验室_污染过程与控制实验室
Affiliation1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China
2.Chinese Acad Sci, Key Lab Expt Marine Biol, Inst Oceanol, Qingdao 266071, Peoples R China
3.SOA, Yantai Ocean Environm Monitoring Cent Stn, Yantai 264006, Peoples R China
First Author AffilicationYantai Institute of Coastal Zone Research, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Li, Chenghua,Wang, Lingling,Ning, Xuanxuan,et al. Identification of two small heat shock proteins with different response profile to cadmium and pathogen stresses in Venerupis philippinarum[J]. CELL STRESS & CHAPERONES,2010,15(6):897-904.
APA Li, Chenghua.,Wang, Lingling.,Ning, Xuanxuan.,Chen, Aiqin.,Zhang, Linbao.,...&Zhao, Jianmin.(2010).Identification of two small heat shock proteins with different response profile to cadmium and pathogen stresses in Venerupis philippinarum.CELL STRESS & CHAPERONES,15(6),897-904.
MLA Li, Chenghua,et al."Identification of two small heat shock proteins with different response profile to cadmium and pathogen stresses in Venerupis philippinarum".CELL STRESS & CHAPERONES 15.6(2010):897-904.
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