| Identification of two small heat shock proteins with different response profile to cadmium and pathogen stresses in Venerupis philippinarum | |
Li, Chenghua1; Wang, Lingling2; Ning, Xuanxuan3; Chen, Aiqin1; Zhang, Linbao1; Qin, Song1 ; Wu, Huifeng1; Zhao, Jianmin1
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| 发表期刊 | CELL STRESS & CHAPERONES
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| ISSN | 1355-8145 |
| 2010-11-01 | |
| 卷号 | 15期号:6页码:897-904 |
| 关键词 | Venerupis Philippinarum Small Heat Shock Protein Time-course Expression Vibrio Anguillarum Cadmium Exposure |
| 产权排序 | [Li, Chenghua; Chen, Aiqin; Zhang, Linbao; Qin, Song; Wu, Huifeng; Zhao, Jianmin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China; [Wang, Lingling] Chinese Acad Sci, Key Lab Expt Marine Biol, Inst Oceanol, Qingdao 266071, Peoples R China; [Ning, Xuanxuan] SOA, Yantai Ocean Environm Monitoring Cent Stn, Yantai 264006, Peoples R China |
| 通讯作者 | Zhao, JM, Chinese Acad Sci, Yantai Inst Coastal Zone Res, 17 Chunhui Rd, Yantai 264003, Peoples R China |
| 作者部门 | 污染过程与控制实验室 |
| 英文摘要 | Small heat shock proteins (sHSPs) encompass a widespread and diverse class of proteins with molecular chaperone activity. In the present study, two sHSP isoforms (VpsHSP-1 and VpsHSP-2) were cloned from Venerupis philippinarum haemocytes by Rapid Amplification of cDNA Ends (RACE) approaches. The expression profiles of these two genes under Vibrio anguillarum challenge and cadmium exposure were investigated by quantitative real-time reverse transcriptase polymerase chain reaction. The bacterial challenge could significantly up-regulate the mRNA expression of both VpsHSP-1 and VpsHSP-2, with the increase of VpsHSP-2 expression occurred earlier than that of VpsHSP-1. During the cadmium exposure experiment, the expression level of both VpsHSP-1 and VpsHSP-2 decreased significantly with larger amplitude in VpsHSP-2. As time progressed, the expression levels of both genes were up-regulated with more increment in the low-chemical exposure groups. The differences in the response to pathogen stimulation and cadmium exposure indicated that there were functional diversity between the two structurally different molecules, VpsHSP-1 and VpsHSP-2, and they probably played distinct roles in mediating the environmental stress and immune responses in calm.; Small heat shock proteins (sHSPs) encompass a widespread and diverse class of proteins with molecular chaperone activity. In the present study, two sHSP isoforms (VpsHSP-1 and VpsHSP-2) were cloned from Venerupis philippinarum haemocytes by Rapid Amplification of cDNA Ends (RACE) approaches. The expression profiles of these two genes under Vibrio anguillarum challenge and cadmium exposure were investigated by quantitative real-time reverse transcriptase polymerase chain reaction. The bacterial challenge could significantly up-regulate the mRNA expression of both VpsHSP-1 and VpsHSP-2, with the increase of VpsHSP-2 expression occurred earlier than that of VpsHSP-1. During the cadmium exposure experiment, the expression level of both VpsHSP-1 and VpsHSP-2 decreased significantly with larger amplitude in VpsHSP-2. As time progressed, the expression levels of both genes were up-regulated with more increment in the low-chemical exposure groups. The differences in the response to pathogen stimulation and cadmium exposure indicated that there were functional diversity between the two structurally different molecules, VpsHSP-1 and VpsHSP-2, and they probably played distinct roles in mediating the environmental stress and immune responses in calm. |
| 文章类型 | Article |
| 资助机构 | Key Laboratory of Experimental Marine Biology (Institute of Oceanology, CAS) ; Chinese Academy of Sciences [kzcx2-yw-225, KZCX2-YW-Q07-04]; NSFC [30901115]; SDSFC [ZR2009CZ008] |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | IMMUNE-RESPONSE ; EXPRESSION ; GENE ; EVOLUTIONARY ; REGULATORS ; INNATE ; ROLES |
| 研究领域[WOS] | Cell Biology |
| WOS记录号 | WOS:000282971000013 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/4415 |
| 专题 | 中国科学院海岸带环境过程与生态修复重点实验室_污染过程与控制实验室 海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室 |
| 作者单位 | 1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China 2.Chinese Acad Sci, Key Lab Expt Marine Biol, Inst Oceanol, Qingdao 266071, Peoples R China 3.SOA, Yantai Ocean Environm Monitoring Cent Stn, Yantai 264006, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Chenghua,Wang, Lingling,Ning, Xuanxuan,et al. Identification of two small heat shock proteins with different response profile to cadmium and pathogen stresses in Venerupis philippinarum[J]. CELL STRESS & CHAPERONES,2010,15(6):897-904. |
| APA | Li, Chenghua.,Wang, Lingling.,Ning, Xuanxuan.,Chen, Aiqin.,Zhang, Linbao.,...&Zhao, Jianmin.(2010).Identification of two small heat shock proteins with different response profile to cadmium and pathogen stresses in Venerupis philippinarum.CELL STRESS & CHAPERONES,15(6),897-904. |
| MLA | Li, Chenghua,et al."Identification of two small heat shock proteins with different response profile to cadmium and pathogen stresses in Venerupis philippinarum".CELL STRESS & CHAPERONES 15.6(2010):897-904. |
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