| Transgenic barley with overexpressed PTrx increases aluminum resistance in roots during germination | |
| Li, Qiao-yun1; Niu, Hong-bin1; Yin, Jun1; Shao, Hong-bo2,3; Niu, Ji-shan1; Ren, Jiang-ping1; Li, Yong-chun1; Wang, Xiang1 | |
| 发表期刊 | JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B
 |
| 2010-11-01 | |
| 卷号 | 11期号:11页码:862-870 |
| 关键词 | Phalaris Coerulescens Thioredoxin Gene (Ptrx) Aluminum Transgenic Barley Oxidative Stress |
| 产权排序 | [Li, Qiao-yun; Niu, Hong-bin; Yin, Jun; Niu, Ji-shan; Ren, Jiang-ping; Li, Yong-chun; Wang, Xiang] Henan Agr Univ, Natl Engn Res Ctr Wheat, Zhengzhou 450002, Peoples R China; [Shao, Hong-bo] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China; [Shao, Hong-bo] Qingdao Univ Sci & Technol, Inst Life Sci, Qingdao 266042, Peoples R China |
| 通讯作者 | Yin, J, Henan Agr Univ, Natl Engn Res Ctr Wheat, Zhengzhou 450002, Peoples R China |
| 作者部门 | 滨海湿地实验室 |
| 英文摘要 | A transgenic barley line (LSY-11-1-1) with overexpressed Phalaris coerulescens thioredoxin gene (PTrx) was employed to measure the growth, protein oxidation, cell viability, and antioxidase activity in barley roots during germination on the presence of 2 mmol/L AlCl3 on filter paper. The results show that (1) compared with the non-transgenic barley, LSY-11-1-1 had enhanced root growth, although both were seriously inhibited after AlCl3 treatment; (2) the degree of protein oxidation and loss of cell viability in roots of LSY-11-1-1 were much less than those in roots of non-transgenic barley, as reflected by lower contents of protein carbonyl and Evans blue uptakes in LSY-11-1-1; (3) activities of catalase (CAT), glutathione peroxidase (GPX), ascorbate peroxidase (APX), and glutathione reductase (GR) in LSY-11-1-1 root tips were generally higher than those in non-transgenic barley root tips, although these antioxidase activities gave a rise to different degrees in both LSY-11-1-1 and non-transgenic barley under aluminum stress. These results indicate that overexpressing PTrx could efficiently protect barley roots from oxidative injury by increasing antioxidase activity, thereby quenching ROS caused by AlCl3 during germination. These properties raise the possibility that transgenic barley with overexpressed PTrx may be used to reduce the aluminum toxicity in acid soils.; A transgenic barley line (LSY-11-1-1) with overexpressed Phalaris coerulescens thioredoxin gene (PTrx) was employed to measure the growth, protein oxidation, cell viability, and antioxidase activity in barley roots during germination on the presence of 2 mmol/L AlCl(3) on filter paper. The results show that (1) compared with the non-transgenic barley, LSY-11-1-1 had enhanced root growth, although both were seriously inhibited after AlCl(3) treatment; (2) the degree of protein oxidation and loss of cell viability in roots of LSY-11-1-1 were much less than those in roots of non-transgenic barley, as reflected by lower contents of protein carbonyl and Evans blue uptakes in LSY-11-1-1; (3) activities of catalase (CAT), glutathione peroxidase (GPX), ascorbate peroxidase (APX), and glutathione reductase (GR) in LSY-11-1-1 root tips were generally higher than those in non-transgenic barley root tips, although these antioxidase activities gave a rise to different degrees in both LSY-11-1-1 and non-transgenic barley under aluminum stress. These results indicate that overexpressing PTrx could efficiently protect barley roots from oxidative injury by increasing antioxidase activity, thereby quenching ROS caused by AlCl(3) during germination. These properties raise the possibility that transgenic barley with overexpressed PTrx may be used to reduce the aluminum toxicity in acid soils. |
| 文章类型 | Article |
| 资助机构 | National Natural Science Foundation of China [30871530]; Science and Technology Transformation Plan of Henan Province [0636000005]; Chinese Academy of Sciences (CAS) ; CAS/SAFEA ; CAS, China [2009Y2B211] |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | OXIDATIVE STRESS ; LIPID-PEROXIDATION ; PLANT THIOREDOXINS ; WHEAT SEEDS ; CELL-DEATH ; TOXICITY ; ANTIOXIDANTS ; TOLERANCE ; RESPONSES ; PROTEINS |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Research & Experimental Medicine |
| WOS记录号 | WOS:000285157500007 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/4409 |
| 专题 | 中国科学院海岸带环境过程与生态修复重点实验室_滨海湿地实验室 |
| 作者单位 | 1.Henan Agr Univ, Natl Engn Res Ctr Wheat, Zhengzhou 450002, Peoples R China 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China 3.Qingdao Univ Sci & Technol, Inst Life Sci, Qingdao 266042, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Qiao-yun,Niu, Hong-bin,Yin, Jun,et al. Transgenic barley with overexpressed PTrx increases aluminum resistance in roots during germination[J]. JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B,2010,11(11):862-870. |
| APA | Li, Qiao-yun.,Niu, Hong-bin.,Yin, Jun.,Shao, Hong-bo.,Niu, Ji-shan.,...&Wang, Xiang.(2010).Transgenic barley with overexpressed PTrx increases aluminum resistance in roots during germination.JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B,11(11),862-870. |
| MLA | Li, Qiao-yun,et al."Transgenic barley with overexpressed PTrx increases aluminum resistance in roots during germination".JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B 11.11(2010):862-870. |
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