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Molecular Characterization of a Novel Big Defensin from Clam Venerupis philippinarum
Zhao, Jianmin1; Li, Chenghua1,2; Chen, Aiqin1; Li, Lingyun3; Su, Xiurong2; Li, Taiwu2
2010-10-20
发表期刊PLOS ONE
ISSN1932-6203
卷号5期号:10页码:e13480-
产权排序[Zhao, Jianmin; Li, Chenghua; Chen, Aiqin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai, Peoples R China; [Li, Chenghua; Su, Xiurong; Li, Taiwu] Ningbo Univ, Fac Life Sci & Biotechnol, Ningbo, Peoples R China; [Li, Lingyun] NE Agr Univ, Coll Anim Sci & Technol, Harbin, Peoples R China
摘要Antimicrobial peptides (AMPs) are important mediators of the primary defense mechanism against microbial invasion. In the present study, a big defensin was identified from Venerupis philippinarum haemocytes (denoted as VpBD) by RACE and EST approaches. The VpBD cDNA contained an open reading frame (ORF) of 285 bp encoding a polypeptide of 94 amino acids. The deduce amino acid sequence of VpBD shared the common features of big defensin including disulfide array organization and helix structure, indicating that VpBD should be a new member of the big defensin family. The mRNA transcript of VpBD was up-regulated significantly during the first 24 hr after Vibrio anguillarum challenge, which was 7.4-fold increase compared to that of the control group. Then the expression decreased gradually from 24 hr to 96 hr, and the lowest expression level was detected at 96 hr post-infection, which was still 3.9-fold higher than that of control. The mature peptide of VpBD was recombined in Escherichia coli and purified for minimum inhibitory concentration (MIC) determination. The rVpBD displayed broad-spectrum inhibitory activity towards all tested bacteria with the highest activity against Staphyloccocus aureus and Pseudomonas putida. These results indicated that VpBD was involved in the host immune response against bacterial infection and might contribute to the clearance of invading bacteria.; Antimicrobial peptides (AMPs) are important mediators of the primary defense mechanism against microbial invasion. In the present study, a big defensin was identified from Venerupis philippinarum haemocytes (denoted as VpBD) by RACE and EST approaches. The VpBD cDNA contained an open reading frame (ORF) of 285 bp encoding a polypeptide of 94 amino acids. The deduce amino acid sequence of VpBD shared the common features of big defensin including disulfide array organization and helix structure, indicating that VpBD should be a new member of the big defensin family. The mRNA transcript of VpBD was up-regulated significantly during the first 24 hr after Vibrio anguillarum challenge, which was 7.4-fold increase compared to that of the control group. Then the expression decreased gradually from 24 hr to 96 hr, and the lowest expression level was detected at 96 hr post-infection, which was still 3.9-fold higher than that of control. The mature peptide of VpBD was recombined in Escherichia coli and purified for minimum inhibitory concentration (MIC) determination. The rVpBD displayed broad-spectrum inhibitory activity towards all tested bacteria with the highest activity against Staphyloccocus aureus and Pseudomonas putida. These results indicated that VpBD was involved in the host immune response against bacterial infection and might contribute to the clearance of invading bacteria.
通讯作者Zhao, JM, Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai, Peoples R China
关键词Oyster Crassostrea-gigas Antimicrobial Peptides Expression Gene Shrimp Family Penaeidins Proteins Mussels Crab
作者部门污染过程与控制实验室 
学科领域Biology ; Multidisciplinary Sciences
项目资助者Chinese Academy of Sciences [kzcx2-yw-225]; NSFC [30901115]
URL查看原文
收录类别SCI
关键词[WOS]OYSTER CRASSOSTREA-GIGAS ; ANTIMICROBIAL PEPTIDES ; EXPRESSION ; GENE ; SHRIMP ; FAMILY ; PENAEIDINS ; PROTEINS ; MUSSELS ; CRAB
文章类型Article
语种英语
WOS研究方向Science & Technology - Other Topics
WOS记录号WOS:000283216400009
引用统计
被引频次:36[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cn/handle/133337/3796
专题污染过程与控制实验室
作者单位1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai, Peoples R China
2.Ningbo Univ, Fac Life Sci & Biotechnol, Ningbo, Peoples R China
3.NE Agr Univ, Coll Anim Sci & Technol, Harbin, Peoples R China
第一作者单位中国科学院烟台海岸带研究所
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Zhao, Jianmin,Li, Chenghua,Chen, Aiqin,et al. Molecular Characterization of a Novel Big Defensin from Clam Venerupis philippinarum[J]. PLOS ONE,2010,5(10):e13480-.
APA Zhao, Jianmin,Li, Chenghua,Chen, Aiqin,Li, Lingyun,Su, Xiurong,&Li, Taiwu.(2010).Molecular Characterization of a Novel Big Defensin from Clam Venerupis philippinarum.PLOS ONE,5(10),e13480-.
MLA Zhao, Jianmin,et al."Molecular Characterization of a Novel Big Defensin from Clam Venerupis philippinarum".PLOS ONE 5.10(2010):e13480-.
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