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Impact of silver nanoparticles on human cells: Effect of particle size
Liu, Wei1; Wu, Yuan1; Wang, Chang1; Li, Hong C.1; Wang, Thanh1; Liao, Chun Y.1,2; Cui, Lin1; Zhou, Qun F.1; Yan, Bing3,4; Jiang, Gui B.1
2010-09-01
发表期刊NANOTOXICOLOGY
ISSN1743-5390
卷号4期号:3页码:319-330
产权排序[Liu, Wei; Wu, Yuan; Wang, Chang; Li, Hong C.; Wang, Thanh; Liao, Chun Y.; Cui, Lin; Zhou, Qun F.; Jiang, Gui B.] Chinese Acad Sci, Ecoenvironm Sci Res Ctr, State Key Lab Environm Chem & Ecotoxicol, Beijing 100085, Peoples R China; [Liao, Chun Y.] Chinese Acad Sci, Yantai Inst Coastal Zone Res Sustainable Dev, Yantai, Shandong, Peoples R China; [Yan, Bing] Shandong Univ, Sch Chem & Chem Engn, Jinan 250100, Peoples R China; [Yan, Bing] St Jude Childrens Hosp, Memphis, TN 38105 USA
摘要This work investigated the cytotoxicities of three silver nanoparticles (SNPs) SNP-5, SNP-20 and SNP-50 with different sizes (similar to 5 nm, similar to 20 nm and similar to 50 nm) using four human cell models (A549, SGC-7901, HepG2 and MCF-7). Endpoints included cell morphology, cell viability, cellular membrane integrity, oxidative stress and cell cycle progression. Observable deleterious effects on the cell morphologies and membrane integrity were induced by SNP-5 and SNP-20. SNPs elevated the ROS levels in cells and arrested the cells at S phase. Apoptosis occurred for 4-9% of the exposed cells. All these cellular responses as well as EC50 values were found to be size-dependent for the tested SNPs. Ultrastructural observations confirmed the presence of SNPs inside cells. Elemental analysis of silver in cells by ICP-MS showed that smaller nanoparticles enter cells more easily than larger ones, which may be the cause of higher toxic effects. The findings may assist in the design of SNP applications and provide insights into their toxicity.; This work investigated the cytotoxicities of three silver nanoparticles (SNPs) SNP-5, SNP-20 and SNP-50 with different sizes (similar to 5 nm, similar to 20 nm and similar to 50 nm) using four human cell models (A549, SGC-7901, HepG2 and MCF-7). Endpoints included cell morphology, cell viability, cellular membrane integrity, oxidative stress and cell cycle progression. Observable deleterious effects on the cell morphologies and membrane integrity were induced by SNP-5 and SNP-20. SNPs elevated the ROS levels in cells and arrested the cells at S phase. Apoptosis occurred for 4-9% of the exposed cells. All these cellular responses as well as EC50 values were found to be size-dependent for the tested SNPs. Ultrastructural observations confirmed the presence of SNPs inside cells. Elemental analysis of silver in cells by ICP-MS showed that smaller nanoparticles enter cells more easily than larger ones, which may be the cause of higher toxic effects. The findings may assist in the design of SNP applications and provide insights into their toxicity.
通讯作者Jiang, GB, Chinese Acad Sci, Ecoenvironm Sci Res Ctr, State Key Lab Environm Chem & Ecotoxicol, POB 2871, Beijing 100085, Peoples R China
关键词Silver Nanoparticles Cytotoxicity Size-dependent
作者部门污染过程与控制实验室 
学科领域Nanoscience & Nanotechnology ; Toxicology
项目资助者National Natural Science Foundation of China [20537020]; Chinese Academy of Sciences [KZCXZ-YW-420-21]; Key Project in the National Science & Technology Pillar Program [2007BAC27B02-1a]
URL查看原文
收录类别SCI
关键词[WOS]IN-VITRO ; GOLD NANOPARTICLES ; CATALYTIC-ACTIVITY ; CELLULAR UPTAKE ; TOXICITY ; CYTOTOXICITY ; NANOSILVER ; MECHANISM ; PULMONARY
文章类型Article
语种英语
WOS研究方向Science & Technology - Other Topics ; Toxicology
WOS记录号WOS:000283555100006
引用统计
被引频次:199[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cn/handle/133337/3792
专题污染过程与控制实验室
作者单位1.Chinese Acad Sci, Ecoenvironm Sci Res Ctr, State Key Lab Environm Chem & Ecotoxicol, Beijing 100085, Peoples R China
2.Chinese Acad Sci, Yantai Inst Coastal Zone Res Sustainable Dev, Yantai, Shandong, Peoples R China
3.Shandong Univ, Sch Chem & Chem Engn, Jinan 250100, Peoples R China
4.St Jude Childrens Hosp, Memphis, TN 38105 USA
推荐引用方式
GB/T 7714
Liu, Wei,Wu, Yuan,Wang, Chang,et al. Impact of silver nanoparticles on human cells: Effect of particle size[J]. NANOTOXICOLOGY,2010,4(3):319-330.
APA Liu, Wei.,Wu, Yuan.,Wang, Chang.,Li, Hong C..,Wang, Thanh.,...&Jiang, Gui B..(2010).Impact of silver nanoparticles on human cells: Effect of particle size.NANOTOXICOLOGY,4(3),319-330.
MLA Liu, Wei,et al."Impact of silver nanoparticles on human cells: Effect of particle size".NANOTOXICOLOGY 4.3(2010):319-330.
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