Impact of silver nanoparticles on human cells: Effect of particle size
Liu, Wei1; Wu, Yuan1; Wang, Chang1; Li, Hong C.1; Wang, Thanh1; Liao, Chun Y.1,2; Cui, Lin1; Zhou, Qun F.1; Yan, Bing3,4; Jiang, Gui B.1
Source PublicationNANOTOXICOLOGY
ISSN1743-5390
2010-09-01
Volume4Issue:3Pages:319-330
KeywordSilver Nanoparticles Cytotoxicity Size-dependent
Contribution Rank[Liu, Wei; Wu, Yuan; Wang, Chang; Li, Hong C.; Wang, Thanh; Liao, Chun Y.; Cui, Lin; Zhou, Qun F.; Jiang, Gui B.] Chinese Acad Sci, Ecoenvironm Sci Res Ctr, State Key Lab Environm Chem & Ecotoxicol, Beijing 100085, Peoples R China; [Liao, Chun Y.] Chinese Acad Sci, Yantai Inst Coastal Zone Res Sustainable Dev, Yantai, Shandong, Peoples R China; [Yan, Bing] Shandong Univ, Sch Chem & Chem Engn, Jinan 250100, Peoples R China; [Yan, Bing] St Jude Childrens Hosp, Memphis, TN 38105 USA
Corresponding AuthorJiang, GB, Chinese Acad Sci, Ecoenvironm Sci Res Ctr, State Key Lab Environm Chem & Ecotoxicol, POB 2871, Beijing 100085, Peoples R China
Department污染过程与控制实验室 
AbstractThis work investigated the cytotoxicities of three silver nanoparticles (SNPs) SNP-5, SNP-20 and SNP-50 with different sizes (similar to 5 nm, similar to 20 nm and similar to 50 nm) using four human cell models (A549, SGC-7901, HepG2 and MCF-7). Endpoints included cell morphology, cell viability, cellular membrane integrity, oxidative stress and cell cycle progression. Observable deleterious effects on the cell morphologies and membrane integrity were induced by SNP-5 and SNP-20. SNPs elevated the ROS levels in cells and arrested the cells at S phase. Apoptosis occurred for 4-9% of the exposed cells. All these cellular responses as well as EC50 values were found to be size-dependent for the tested SNPs. Ultrastructural observations confirmed the presence of SNPs inside cells. Elemental analysis of silver in cells by ICP-MS showed that smaller nanoparticles enter cells more easily than larger ones, which may be the cause of higher toxic effects. The findings may assist in the design of SNP applications and provide insights into their toxicity.; This work investigated the cytotoxicities of three silver nanoparticles (SNPs) SNP-5, SNP-20 and SNP-50 with different sizes (similar to 5 nm, similar to 20 nm and similar to 50 nm) using four human cell models (A549, SGC-7901, HepG2 and MCF-7). Endpoints included cell morphology, cell viability, cellular membrane integrity, oxidative stress and cell cycle progression. Observable deleterious effects on the cell morphologies and membrane integrity were induced by SNP-5 and SNP-20. SNPs elevated the ROS levels in cells and arrested the cells at S phase. Apoptosis occurred for 4-9% of the exposed cells. All these cellular responses as well as EC50 values were found to be size-dependent for the tested SNPs. Ultrastructural observations confirmed the presence of SNPs inside cells. Elemental analysis of silver in cells by ICP-MS showed that smaller nanoparticles enter cells more easily than larger ones, which may be the cause of higher toxic effects. The findings may assist in the design of SNP applications and provide insights into their toxicity.
Funding OrganizationNational Natural Science Foundation of China [20537020]; Chinese Academy of Sciences [KZCXZ-YW-420-21]; Key Project in the National Science & Technology Pillar Program [2007BAC27B02-1a]
SubtypeArticle
Indexed BySCI
Language英语
WOS KeywordIN-VITRO ; GOLD NANOPARTICLES ; CATALYTIC-ACTIVITY ; CELLULAR UPTAKE ; TOXICITY ; CYTOTOXICITY ; NANOSILVER ; MECHANISM ; PULMONARY
WOS Research AreaScience & Technology - Other Topics ; Toxicology
WOS IDWOS:000283555100006
Citation statistics
Cited Times:289[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.yic.ac.cn/handle/133337/3791
Collection中科院海岸带环境过程与生态修复重点实验室_污染过程与控制实验室
Affiliation1.Chinese Acad Sci, Ecoenvironm Sci Res Ctr, State Key Lab Environm Chem & Ecotoxicol, Beijing 100085, Peoples R China
2.Chinese Acad Sci, Yantai Inst Coastal Zone Res Sustainable Dev, Yantai, Shandong, Peoples R China
3.Shandong Univ, Sch Chem & Chem Engn, Jinan 250100, Peoples R China
4.St Jude Childrens Hosp, Memphis, TN 38105 USA
Recommended Citation
GB/T 7714
Liu, Wei,Wu, Yuan,Wang, Chang,et al. Impact of silver nanoparticles on human cells: Effect of particle size[J]. NANOTOXICOLOGY,2010,4(3):319-330.
APA Liu, Wei.,Wu, Yuan.,Wang, Chang.,Li, Hong C..,Wang, Thanh.,...&Jiang, Gui B..(2010).Impact of silver nanoparticles on human cells: Effect of particle size.NANOTOXICOLOGY,4(3),319-330.
MLA Liu, Wei,et al."Impact of silver nanoparticles on human cells: Effect of particle size".NANOTOXICOLOGY 4.3(2010):319-330.
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