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Determination of critical aggregation concentration and aggregation number of acid-soluble collagen from walleye pollock (Theragra chalcogramma) skin using the fluorescence probe pyrene
Yan, Mingyan1,2; Li, Bafang1; Zhao, Xue1
2010-10-15
Source PublicationFOOD CHEMISTRY
ISSN0308-8146
Volume122Issue:4Pages:1333-1337
Contribution Rank[Yan, Mingyan; Li, Bafang; Zhao, Xue] Ocean Univ China, Coll Food Sci & Technol, Qingdao 266003, Shandong, Peoples R China; [Yan, Mingyan] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Shandong, Peoples R China
AbstractThe aim of this paper was to investigate the critical aggregation concentration and aggregation number of acid-soluble collagen from walleye pollock (Theragra chalcogramma) skin using the fluorescence probe pyrene. Results showed that pyrene was fit for studying the aggregation behaviour of collagen in sodium phosphate buffer at pH 7.2. The plots of the pyrene l(1)/l(3) ratio, as a function of the logarithm of total collagen concentration, revealed a typical sigmoidal decrease, the critical aggregation concentration (CAC) from which was determined to be at 0.48 mg/ml. The subsequent transient fluorescence decay study indicated that the aggregation number of collagen was not constant, but varied with different concentrations of collagen. The structure of the aggregates tended to be stable, when the collagen concentration exceeded 1.2 mg/ml. (C) 2010 Elsevier Ltd. All rights reserved.; The aim of this paper was to investigate the critical aggregation concentration and aggregation number of acid-soluble collagen from walleye pollock (Theragra chalcogramma) skin using the fluorescence probe pyrene. Results showed that pyrene was fit for studying the aggregation behaviour of collagen in sodium phosphate buffer at pH 7.2. The plots of the pyrene l(1)/l(3) ratio, as a function of the logarithm of total collagen concentration, revealed a typical sigmoidal decrease, the critical aggregation concentration (CAC) from which was determined to be at 0.48 mg/ml. The subsequent transient fluorescence decay study indicated that the aggregation number of collagen was not constant, but varied with different concentrations of collagen. The structure of the aggregates tended to be stable, when the collagen concentration exceeded 1.2 mg/ml. (C) 2010 Elsevier Ltd. All rights reserved.
Corresponding AuthorLi, BF, Ocean Univ China, Coll Food Sci & Technol, 5 Yushan Rd, Qingdao 266003, Shandong, Peoples R China
KeywordCollagen Pyrene Aggregation Walleye Pollock Fish Skin
Department生物资源实验室 
Subject AreaChemistry, Applied ; Food Science & Technology ; Nutrition & Dietetics
Funding OrganizationNational Natural Science Foundation of China [30871943]; High Technology Research and Development Programme of China [2006AA09Z438]
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Indexed BySCI
WOS KeywordCRITICAL MICELLE CONCENTRATION ; AQUEOUS-SOLUTION ; SURFACTANT
SubtypeArticle
Language英语
WOS Research AreaChemistry ; Food Science & Technology ; Nutrition & Dietetics
WOS IDWOS:000278582400062
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Document Type期刊论文
Identifierhttp://ir.yic.ac.cn/handle/133337/3751
Collection海岸带生物学与生物资源保护实验室
Affiliation1.Ocean Univ China, Coll Food Sci & Technol, Qingdao 266003, Shandong, Peoples R China
2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Shandong, Peoples R China
Recommended Citation
GB/T 7714
Yan, Mingyan,Li, Bafang,Zhao, Xue. Determination of critical aggregation concentration and aggregation number of acid-soluble collagen from walleye pollock (Theragra chalcogramma) skin using the fluorescence probe pyrene[J]. FOOD CHEMISTRY,2010,122(4):1333-1337.
APA Yan, Mingyan,Li, Bafang,&Zhao, Xue.(2010).Determination of critical aggregation concentration and aggregation number of acid-soluble collagen from walleye pollock (Theragra chalcogramma) skin using the fluorescence probe pyrene.FOOD CHEMISTRY,122(4),1333-1337.
MLA Yan, Mingyan,et al."Determination of critical aggregation concentration and aggregation number of acid-soluble collagen from walleye pollock (Theragra chalcogramma) skin using the fluorescence probe pyrene".FOOD CHEMISTRY 122.4(2010):1333-1337.
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