| Determination of critical aggregation concentration and aggregation number of acid-soluble collagen from walleye pollock (Theragra chalcogramma) skin using the fluorescence probe pyrene | |
Yan, Mingyan1,2 ; Li, Bafang1; Zhao, Xue1
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| 2010-10-15 | |
| Source Publication | FOOD CHEMISTRY
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| ISSN | 0308-8146 |
| Volume | 122Issue:4Pages:1333-1337 |
| Contribution Rank | [Yan, Mingyan; Li, Bafang; Zhao, Xue] Ocean Univ China, Coll Food Sci & Technol, Qingdao 266003, Shandong, Peoples R China; [Yan, Mingyan] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Shandong, Peoples R China |
| Abstract | The aim of this paper was to investigate the critical aggregation concentration and aggregation number of acid-soluble collagen from walleye pollock (Theragra chalcogramma) skin using the fluorescence probe pyrene. Results showed that pyrene was fit for studying the aggregation behaviour of collagen in sodium phosphate buffer at pH 7.2. The plots of the pyrene l(1)/l(3) ratio, as a function of the logarithm of total collagen concentration, revealed a typical sigmoidal decrease, the critical aggregation concentration (CAC) from which was determined to be at 0.48 mg/ml. The subsequent transient fluorescence decay study indicated that the aggregation number of collagen was not constant, but varied with different concentrations of collagen. The structure of the aggregates tended to be stable, when the collagen concentration exceeded 1.2 mg/ml. (C) 2010 Elsevier Ltd. All rights reserved.; The aim of this paper was to investigate the critical aggregation concentration and aggregation number of acid-soluble collagen from walleye pollock (Theragra chalcogramma) skin using the fluorescence probe pyrene. Results showed that pyrene was fit for studying the aggregation behaviour of collagen in sodium phosphate buffer at pH 7.2. The plots of the pyrene l(1)/l(3) ratio, as a function of the logarithm of total collagen concentration, revealed a typical sigmoidal decrease, the critical aggregation concentration (CAC) from which was determined to be at 0.48 mg/ml. The subsequent transient fluorescence decay study indicated that the aggregation number of collagen was not constant, but varied with different concentrations of collagen. The structure of the aggregates tended to be stable, when the collagen concentration exceeded 1.2 mg/ml. (C) 2010 Elsevier Ltd. All rights reserved. |
| Corresponding Author | Li, BF, Ocean Univ China, Coll Food Sci & Technol, 5 Yushan Rd, Qingdao 266003, Shandong, Peoples R China |
| Keyword | Collagen Pyrene Aggregation Walleye Pollock Fish Skin |
| Department | 生物资源实验室 |
| Subject Area | Chemistry, Applied ; Food Science & Technology ; Nutrition & Dietetics |
| Funding Organization | National Natural Science Foundation of China [30871943]; High Technology Research and Development Programme of China [2006AA09Z438] |
| URL | 查看原文 |
| Indexed By | SCI |
| WOS Keyword | CRITICAL MICELLE CONCENTRATION ; AQUEOUS-SOLUTION ; SURFACTANT |
| Subtype | Article |
| Language | 英语 |
| WOS Research Area | Chemistry ; Food Science & Technology ; Nutrition & Dietetics |
| WOS ID | WOS:000278582400062 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.yic.ac.cn/handle/133337/3751 |
| Collection | 海岸带生物学与生物资源保护实验室 |
| Affiliation | 1.Ocean Univ China, Coll Food Sci & Technol, Qingdao 266003, Shandong, Peoples R China 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Shandong, Peoples R China |
| First Author Affilication | Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences |
| Recommended Citation GB/T 7714 | Yan, Mingyan,Li, Bafang,Zhao, Xue. Determination of critical aggregation concentration and aggregation number of acid-soluble collagen from walleye pollock (Theragra chalcogramma) skin using the fluorescence probe pyrene[J]. FOOD CHEMISTRY,2010,122(4):1333-1337. |
| APA | Yan, Mingyan,Li, Bafang,&Zhao, Xue.(2010).Determination of critical aggregation concentration and aggregation number of acid-soluble collagen from walleye pollock (Theragra chalcogramma) skin using the fluorescence probe pyrene.FOOD CHEMISTRY,122(4),1333-1337. |
| MLA | Yan, Mingyan,et al."Determination of critical aggregation concentration and aggregation number of acid-soluble collagen from walleye pollock (Theragra chalcogramma) skin using the fluorescence probe pyrene".FOOD CHEMISTRY 122.4(2010):1333-1337. |
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