Determination of critical aggregation concentration and aggregation number of acid-soluble collagen from walleye pollock (Theragra chalcogramma) skin using the fluorescence probe pyrene
Yan, Mingyan1,2; Li, Bafang1; Zhao, Xue1
2010-10-15
发表期刊FOOD CHEMISTRY
ISSN0308-8146
卷号122期号:4页码:1333-1337
产权排序[Yan, Mingyan; Li, Bafang; Zhao, Xue] Ocean Univ China, Coll Food Sci & Technol, Qingdao 266003, Shandong, Peoples R China; [Yan, Mingyan] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Shandong, Peoples R China
摘要The aim of this paper was to investigate the critical aggregation concentration and aggregation number of acid-soluble collagen from walleye pollock (Theragra chalcogramma) skin using the fluorescence probe pyrene. Results showed that pyrene was fit for studying the aggregation behaviour of collagen in sodium phosphate buffer at pH 7.2. The plots of the pyrene l(1)/l(3) ratio, as a function of the logarithm of total collagen concentration, revealed a typical sigmoidal decrease, the critical aggregation concentration (CAC) from which was determined to be at 0.48 mg/ml. The subsequent transient fluorescence decay study indicated that the aggregation number of collagen was not constant, but varied with different concentrations of collagen. The structure of the aggregates tended to be stable, when the collagen concentration exceeded 1.2 mg/ml. (C) 2010 Elsevier Ltd. All rights reserved.; The aim of this paper was to investigate the critical aggregation concentration and aggregation number of acid-soluble collagen from walleye pollock (Theragra chalcogramma) skin using the fluorescence probe pyrene. Results showed that pyrene was fit for studying the aggregation behaviour of collagen in sodium phosphate buffer at pH 7.2. The plots of the pyrene l(1)/l(3) ratio, as a function of the logarithm of total collagen concentration, revealed a typical sigmoidal decrease, the critical aggregation concentration (CAC) from which was determined to be at 0.48 mg/ml. The subsequent transient fluorescence decay study indicated that the aggregation number of collagen was not constant, but varied with different concentrations of collagen. The structure of the aggregates tended to be stable, when the collagen concentration exceeded 1.2 mg/ml. (C) 2010 Elsevier Ltd. All rights reserved.
通讯作者Li, BF, Ocean Univ China, Coll Food Sci & Technol, 5 Yushan Rd, Qingdao 266003, Shandong, Peoples R China
关键词Collagen Pyrene Aggregation Walleye Pollock Fish Skin
作者部门生物资源实验室 
学科领域Chemistry, Applied ; Food Science & Technology ; Nutrition & Dietetics
项目资助者National Natural Science Foundation of China [30871943]; High Technology Research and Development Programme of China [2006AA09Z438]
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收录类别SCI
关键词[WOS]CRITICAL MICELLE CONCENTRATION ; AQUEOUS-SOLUTION ; SURFACTANT
文章类型Article
语种英语
WOS研究方向Chemistry ; Food Science & Technology ; Nutrition & Dietetics
WOS记录号WOS:000278582400062
引用统计
文献类型期刊论文
条目标识符http://ir.yic.ac.cn/handle/133337/3751
专题海岸带生物学与生物资源利用所重点实验室
作者单位1.Ocean Univ China, Coll Food Sci & Technol, Qingdao 266003, Shandong, Peoples R China
2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Shandong, Peoples R China
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Yan, Mingyan,Li, Bafang,Zhao, Xue. Determination of critical aggregation concentration and aggregation number of acid-soluble collagen from walleye pollock (Theragra chalcogramma) skin using the fluorescence probe pyrene[J]. FOOD CHEMISTRY,2010,122(4):1333-1337.
APA Yan, Mingyan,Li, Bafang,&Zhao, Xue.(2010).Determination of critical aggregation concentration and aggregation number of acid-soluble collagen from walleye pollock (Theragra chalcogramma) skin using the fluorescence probe pyrene.FOOD CHEMISTRY,122(4),1333-1337.
MLA Yan, Mingyan,et al."Determination of critical aggregation concentration and aggregation number of acid-soluble collagen from walleye pollock (Theragra chalcogramma) skin using the fluorescence probe pyrene".FOOD CHEMISTRY 122.4(2010):1333-1337.
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