The biosynthetic pathway of carotenoids in the astaxanthin-producing green alga Chlorella zofingiensis

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	The biosynthetic pathway of carotenoids in the astaxanthin-producing green alga Chlorella zofingiensis
	Wang, Yan1,2 ; Chen, Tao 2
发表期刊	WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
ISSN	0959-3993
	2008-12-01
卷号	24 期号:12 页码:2927-2932
关键词	Astaxanthin Chlorella Zofingiensis Diphenylamine Zeaxanthin Glucose Stress
产权排序	Chinese Acad Sci, Yantai Inst Coastal Zone Res Sustainable Dev;Chinese Acad Sci, S China Sea Inst Oceanol
通讯作者	Wang, Y, Chinese Acad Sci, Yantai Inst Coastal Zone Res Sustainable Dev, Yantai 264003, Shandong, Peoples R China
作者部门	生物资源实验室
英文摘要	The carotenoid composition of the astaxanthin-producing green alga Chlorella zofingiensis was investigated using high-performance liquid chromatography. Astaxanthin, adonixanthin, and zeaxanthin are the major carotenoids in this alga. The pigment pattern was characterized during the accumulation period, and in response to diphenylamine (DPA), an inhibitor of carotenoid biosynthesis. An increase in zeaxanthin followed by a decrease in xanthophyll was seen after the induction of astaxanthin biosynthesis by glucose. This biphasic kinetics of zeaxanthin was parallel to the marked increase in adonixanthin (from 0 mg g(-1) to 0.21 mg g(-1)) and astaxanthin (from 0.05 mg g(-1) to 0.35 mg g(-1)) and decrease of beta-carotene (from 0.30 mg g(-1) to 0.03 mg g(-1)). More importantly, unlike the Haematococcus alga, in which there was a high beta-carotene accumulation after DPA treatment, C. zofingiensis showed an accumulation of extra zeaxanthin instead of beta-carotene after treatment of the cells with DPA. All these results observed in vivo studies corroborate the observations in vitro studies at the enzyme level that zeaxanthin is a substrate for the carotenoid oxygenase in C. zofingiensis. It is suggested that zeaxanthin might be an important intermediate and not an end product of the biosynthetic pathway of astaxanthin. Therefore, a new pathway for astaxanthin formation by C. zofingiensis, which is different from that of the other astaxanthin-producing microorganisms, is proposed. An understanding of the astaxanthin biosynthetic pathway may yield important information toward the optimization of astaxanthin production, especially for the improvement of astaxanthin through genetic manipulations.
文章类型	Article
资助机构	YICCAS [A3J0708DB-010]; Sci-tech Development Grant of Shandong [2007GG2QT06020]
收录类别	SCI
语种	英语
关键词[WOS]	HAEMATOCOCCUS-PLUVIALIS ; XANTHOPHYLL CYCLE ; CHLOROPHYTA ; INHIBITION ; MICROALGAE ; NUTRITION ; STRESS ; GENE
研究领域[WOS]	Biotechnology & Applied Microbiology
WOS记录号	WOS:000260319900023
引用统计	被引频次：27[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/3495
专题	海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
作者单位	1.Chinese Acad Sci, Yantai Inst Coastal Zone Res Sustainable Dev, Yantai 264003, Shandong, Peoples R China 2.Chinese Acad Sci, S China Sea Inst Oceanol, Guangzhou, Guangdong, Peoples R China
推荐引用方式 GB/T 7714	Wang, Yan,Chen, Tao. The biosynthetic pathway of carotenoids in the astaxanthin-producing green alga Chlorella zofingiensis[J]. WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY,2008,24(12):2927-2932.
APA	Wang, Yan,&Chen, Tao.(2008).The biosynthetic pathway of carotenoids in the astaxanthin-producing green alga Chlorella zofingiensis.WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY,24(12),2927-2932.
MLA	Wang, Yan,et al."The biosynthetic pathway of carotenoids in the astaxanthin-producing green alga Chlorella zofingiensis".WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY 24.12(2008):2927-2932.

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