Sugar-based growth, astaxanthin accumulation and carotenogenic transcription of heterotrophic Chlorella zofingiensis (Chlorophyta)
Sun, Ni1,2; Wang, Yan2,3; Li, Yan-Tao1; Huang, Jun-Chao1; Chen, Feng1
发表期刊PROCESS BIOCHEMISTRY
ISSN1359-5113
2008-11-01
卷号43期号:11页码:1288-1292
关键词Astaxanthin Beta-carotene Ketolase Beta-carotene Hydroxylase Chlorella Zofingiensis Heterotrophic Sugar
产权排序Univ Hong Kong, Sch Biol Sci;Chinese Acad Sci, S China Sea Inst Oceanol;Chinese Acad Sci, Yantai Inst Coastal Zone Res Sustainable Dev
通讯作者Huang, JC, Univ Hong Kong, Sch Biol Sci, Pokfulam Road, Hong Kong, Hong Kong, Peoples R China
作者部门生物资源实验室
英文摘要The green microalga Chlorella zofingiensis can grow and produce the ketocarotenoid astaxanthin in the dark with glucose as sole carbon and energy source. In the present study, we reported that glucose, mannose, fructose, sucrose, galactose and lactose could differentially support the cell growth and astaxanthin biosynthesis. Of the sugars surveyed, glucose and mannose were the best carbon sources for the algal growth in the dark, as indicated by the relatively high specific growth rates (ca. 0.03 h(-1)) and high cell dry densities (ca. 10 g l(-1)). Furthermore, the algal cells cultured with glucose and mannose accumulated the highest amounts of astaxanthin (ca. 1 mg g(-1)), indicating a correlation between cell growth and astaxanthin formation. In addition, various sugars differentially regulated the transcription of three carotenogenic genes encoding phytoene desaturase (PDS), beta-carotene ketolase (BKT), and beta-carotene hydroxylase (CHYb) respectively. By using glucose fed-batch fermentation, high cell dry weight concentration (ca. 53 g l(-1)) and high astaxanthin production (ca. 32 mg l(-1)) were obtained, which are much higher than those ever reported in the alga. The present study suggests that C. zofingiensis is suitable for the production of natural astaxanthin on a massive scale. (C) 2008 Elsevier Ltd. All rights reserved.
文章类型Article
资助机构Research Grants Council of the Hong Kong Special Administrative Region, China [HKU 7623/05M]
收录类别SCI
语种英语
关键词[WOS]SECONDARY CAROTENOIDS ; GLUCOSE ; CULTURE ; MICROALGAE ; PLUVIALIS ; CLONING ; SYSTEM ; OXYGEN ; PLANTS ; CELLS
研究领域[WOS]Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Engineering
WOS记录号WOS:000260809300016
引用统计
被引频次:134[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cn/handle/133337/3493
专题海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
作者单位1.Univ Hong Kong, Sch Biol Sci, Hong Kong, Hong Kong, Peoples R China
2.Chinese Acad Sci, S China Sea Inst Oceanol, Guangzhou, Guangdong, Peoples R China
3.Chinese Acad Sci, Yantai Inst Coastal Zone Res Sustainable Dev, Yantai, Peoples R China
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GB/T 7714
Sun, Ni,Wang, Yan,Li, Yan-Tao,et al. Sugar-based growth, astaxanthin accumulation and carotenogenic transcription of heterotrophic Chlorella zofingiensis (Chlorophyta)[J]. PROCESS BIOCHEMISTRY,2008,43(11):1288-1292.
APA Sun, Ni,Wang, Yan,Li, Yan-Tao,Huang, Jun-Chao,&Chen, Feng.(2008).Sugar-based growth, astaxanthin accumulation and carotenogenic transcription of heterotrophic Chlorella zofingiensis (Chlorophyta).PROCESS BIOCHEMISTRY,43(11),1288-1292.
MLA Sun, Ni,et al."Sugar-based growth, astaxanthin accumulation and carotenogenic transcription of heterotrophic Chlorella zofingiensis (Chlorophyta)".PROCESS BIOCHEMISTRY 43.11(2008):1288-1292.
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