Molecular cloning and stress-dependent expression of a gene encoding Delta(12)-fatty acid desaturase in the Antarctic microalga Chlorella vulgaris NJ-7
Lu, Yandu1,2,3; Chi, Xiaoyuan4; Yang, Qingli4; Li, Zhaoxin5; Liu, Shaofang2,3; Gan, Qinhua6; Qin, Song1
发表期刊EXTREMOPHILES
ISSN1431-0651
2009-11-01
卷号13期号:6页码:875-884
关键词Cold Stress High Salinity Stress Antarctic Delta(12) Fatty Acid Desaturases
产权排序Chinese Acad Sci, Yantai Inst Coastal Zone Res Sustainable Dev;Chinese Acad Sci, Inst Oceanol;Chinese Acad Sci, Grad Sch;Shandong Peanut Res Inst;Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst;Shandong Entry Exit Inspect & Quarantine Bur
通讯作者Qin, S, Chinese Acad Sci, Yantai Inst Coastal Zone Res Sustainable Dev, Yantai 264003, Peoples R China
作者部门生物资源实验室
英文摘要The psychrotrophic Antarctic alga, Chlorella vulgaris NJ-7, grows under an extreme environment of low temperature and high salinity. In an effort to better understand the correlation between fatty acid metabolism and acclimation to Antarctic environment, we analyzed its fatty acid compositions. An extremely high amount of Delta(12) unsaturated fatty acids was identified which prompted us to speculate about the involvement of Delta(12) fatty acid desaturase in the process of acclimation. A full-length cDNA sequence, designated CvFAD2, was isolated from C. vulgaris NJ-7 via reverse transcription polymerase chain reaction (RT-PCR) and RACE methods. Sequence alignment and phylogenetic analysis showed that the gene was homologous to known microsomal Delta(12)-FADs with the conserved histidine motifs. Heterologous expression in yeast was used to confirm the regioselectivity and the function of CvFAD2. Linoleic acid (18:2), normally not present in wild-type yeast cells, was detected in transformants of CvFAD2. The induction of CvFAD2 at an mRNA level under cold stress and high salinity is detected by real-time PCR. The results showed that both temperature and salinity motivated the upregulation of CvFAD2 expression. The accumulation of CvFAD2 increased 2.2-fold at 15A degrees C and 3.9-fold at 4A degrees C compared to the alga at 25A degrees C. Meanwhile a 1.7- and 8.5-fold increase at 3 and 6% NaCl was detected. These data suggest that CvFAD2 is the enzyme responsible for the Delta(12) fatty acids desaturation involved in the adaption to cold and high salinity for Antarctic C. vugaris NJ-7.
文章类型Article
资助机构National High-Tech Research and Development Plan of China [2006AA10A114]; National Key Basic Research and Development Project of China [2007CB116212]; Key Innovative Project of Chinese Academy of Science [KSCX2-YW-G-002, KZCX2-YW-225]; National Natural Sc
收录类别SCI
语种英语
关键词[WOS]MULTIPLE SEQUENCE ALIGNMENT ; FUNCTIONAL-CHARACTERIZATION ; MEMBRANE-FLUIDITY ; PAVLOVA-SALINA ; SPLICE SITES ; YEAST ; IDENTIFICATION ; ENZYME ; CHLOROPHYCEAE ; BIOSYNTHESIS
研究领域[WOS]Biochemistry & Molecular Biology ; Microbiology
WOS记录号WOS:000271199500003
引用统计
被引频次:43[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cn/handle/133337/3475
专题海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
作者单位1.Chinese Acad Sci, Yantai Inst Coastal Zone Res Sustainable Dev, Yantai 264003, Peoples R China
2.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
3.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
4.Shandong Peanut Res Inst, Qingdao 266100, Peoples R China
5.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China
6.Shandong Entry Exit Inspect & Quarantine Bur, Qingdao 266001, Peoples R China
推荐引用方式
GB/T 7714
Lu, Yandu,Chi, Xiaoyuan,Yang, Qingli,et al. Molecular cloning and stress-dependent expression of a gene encoding Delta(12)-fatty acid desaturase in the Antarctic microalga Chlorella vulgaris NJ-7[J]. EXTREMOPHILES,2009,13(6):875-884.
APA Lu, Yandu.,Chi, Xiaoyuan.,Yang, Qingli.,Li, Zhaoxin.,Liu, Shaofang.,...&Qin, Song.(2009).Molecular cloning and stress-dependent expression of a gene encoding Delta(12)-fatty acid desaturase in the Antarctic microalga Chlorella vulgaris NJ-7.EXTREMOPHILES,13(6),875-884.
MLA Lu, Yandu,et al."Molecular cloning and stress-dependent expression of a gene encoding Delta(12)-fatty acid desaturase in the Antarctic microalga Chlorella vulgaris NJ-7".EXTREMOPHILES 13.6(2009):875-884.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
Molecular cloning an(387KB) 开放获取--浏览 请求全文
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Lu, Yandu]的文章
[Chi, Xiaoyuan]的文章
[Yang, Qingli]的文章
百度学术
百度学术中相似的文章
[Lu, Yandu]的文章
[Chi, Xiaoyuan]的文章
[Yang, Qingli]的文章
必应学术
必应学术中相似的文章
[Lu, Yandu]的文章
[Chi, Xiaoyuan]的文章
[Yang, Qingli]的文章
相关权益政策
暂无数据
收藏/分享
文件名: Molecular cloning and stress-dependent expression of a gene encoding Delta(12)-fatty acid desaturase in the Antarctic microalga Chlorella vulgaris NJ-7.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。