| A sensitive immunosensor using colloidal gold as electrochemical label | |
Chen, Zhao-Peng ; Peng, Zhao-Feng; Zhang, Peng; Jin, Xue-Fang; Jiang, Jian-Hui; Zhang, Xiao-Bing; Shen, Guo-Li; Yu, Ru-Qin
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| 发表期刊 | TALANTA
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| 2007-07-31 | |
| 卷号 | 72期号:5页码:1800-1804 |
| 关键词 | Immunosensor Colloidal Gold Electrochemical Label Adsorptive Voltammetry |
| 产权排序 | Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr; Hunan Normal Univ, Minist Educ, Chem & Chem Engn Coll, Key Lab Chem Biol & Tradit Chinese Med Res; Chinese Acad Sci, Yantai Inst Coastal Zone Res Sustainable Dev, Dept Environm Chem. |
| 通讯作者 | Yu, RQ |
| 作者部门 | 环境化学实验室 |
| 英文摘要 | A sensitive immunosensor using colloidal gold as electrochemical label is described. In this method, the capture protein was first immobilized on a carbon paste electrode surface through passive adsorption to bind quantitatively with corresponding antigen and colloidal gold labeled antibody to perform a sandwich assay. To detect the amount of the colloidal gold captured on the electrode surface, the colloid was first oxidized electrochemically to produce AuCl4- ions which were adsorbed strongly on the electrode surface. Adsorptive voltammetry was then employed for the determination of the adsorbed AuCl4- ions. A linear relationship between reduction wave peak cur-rent and the antigen concentration (human IgG) from 10 to 500 ng/ml is obtained with a detection limit of 4.0 ng/ml. (C) 2007 Elsevier B.V. All rights reserved. |
| 文章类型 | Article |
| 收录类别 | ISTP ; SCI |
| 语种 | 英语 |
| 关键词[WOS] | SURFACE-PLASMON RESONANCE ; ELECTRON-MICROSCOPE ; AU ; BIOSENSOR |
| 研究领域[WOS] | Chemistry |
| WOS记录号 | WOS:000248528100033 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/3459 |
| 专题 | 中国科学院海岸带环境过程与生态修复重点实验室_海岸带环境工程技术研究与发展中心 中国科学院海岸带环境过程与生态修复重点实验室_海岸带环境过程实验室 |
| 作者单位 | 1.Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Peoples R China 2.Hunan Normal Univ, Minist Educ, Chem & Chem Engn Coll, Key Lab Chem Biol & Tradit Chinese Med Res, Changsha 410081, Peoples R China 3.Chinese Acad Sci, Yantai Inst Coastal Zone Res Sustainable Dev, Dept Environm Chem, Yantai 264003, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Zhao-Peng,Peng, Zhao-Feng,Zhang, Peng,et al. A sensitive immunosensor using colloidal gold as electrochemical label[J]. TALANTA,2007,72(5):1800-1804. |
| APA | Chen, Zhao-Peng.,Peng, Zhao-Feng.,Zhang, Peng.,Jin, Xue-Fang.,Jiang, Jian-Hui.,...&Yu, Ru-Qin.(2007).A sensitive immunosensor using colloidal gold as electrochemical label.TALANTA,72(5),1800-1804. |
| MLA | Chen, Zhao-Peng,et al."A sensitive immunosensor using colloidal gold as electrochemical label".TALANTA 72.5(2007):1800-1804. |
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