An electrochemical amplification immunoassay using biocatalytic metal deposition coupled with anodic stripping voltammetric detection
Chen, Zhao-Peng1,2; Peng, Zhao-Feng1; Jiang, Jian-Hui1; Zhang, Xiao-Bing1; Shen, Guo-Li1; Yu, Ru-Qin1
发表期刊SENSORS AND ACTUATORS B-CHEMICAL
ISSN0925-4005
2008-01-29
卷号129期号:1页码:146-151
关键词Electrochemical Immunoassay Anodic Stripping Voltammmetry Biocatalytic Metal Deposition
产权排序Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr; Chinese Acad Sci, Dept Environm Chem, Yantai Inst Coastal Zone Res Sustainable Dev
通讯作者Yu, RQ, Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Peoples R China
作者部门环境化学实验室
英文摘要An electrochemical amplification immunoassay is reported using biocatalytic metal deposition coupled with anodic stripping voltammetric detection. In this method, the captured antibody was first immobilized onto a gold electrode via a self-assembled layer. After a sandwich immunoreaction, alkaline phosphatase-labeled antibody was bound to the gold electrode. The alkaline phosphatase on the electrode catalyzes the hydrolysis of ascorbic acid 2-phosphate to produce ascorbic acid. The latter, in turn, reduced silver ions on the electrode surface, leading to the deposition of silver onto the protein-modified electrode surface. The deposited metal was electrochemically stripped into solution and then measured by anodic stripping voltammmetry. Compared with the direct voltammetric detection of ascorbic acid, anodic stripping voltammetric detection of metal ions is more sensitive. For the amount of deposited silver relates to the amount of enzyme-generated ascorbic acid, which was controlled by the amount of enzyme bound on the electrode surface, the stripping current signal reflects the amount of target protein, achieving a linearly relationship in the range from 5 to 1000 ng mL(-1) in a logarithmic plot with a detection limit of 2.2 ng mL(-1). The utilization of the high biocatalytic activity of enzyme and the sensitive anodic stripping voltammetry to detect metal ions dramatically enhanced the sensitivity in immunoassay. (C) 2007 Elsevier B.V. All rights reserved.
文章类型Article
收录类别SCI
语种英语
关键词[WOS]GOLD NANOPARTICLE LABEL ; DNA HYBRIDIZATION ; AMPEROMETRIC IMMUNOSENSORS ; HORSERADISH-PEROXIDASE ; ENZYME-IMMUNOASSAY ; LIPOSOMES ; TRANSDUCTION ; ELECTRODES
研究领域[WOS]Chemistry ; Electrochemistry ; Instruments & Instrumentation
WOS记录号WOS:000253172500023
引用统计
被引频次:24[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cn/handle/133337/3455
专题中国科学院海岸带环境过程与生态修复重点实验室_海岸带环境工程技术研究与发展中心
中国科学院海岸带环境过程与生态修复重点实验室_海岸带环境过程实验室
作者单位1.Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Peoples R China
2.Chinese Acad Sci, Dept Environm Chem, Yantai Inst Coastal Zone Res Sustainable Dev, Yantai 264003, Peoples R China
推荐引用方式
GB/T 7714
Chen, Zhao-Peng,Peng, Zhao-Feng,Jiang, Jian-Hui,et al. An electrochemical amplification immunoassay using biocatalytic metal deposition coupled with anodic stripping voltammetric detection[J]. SENSORS AND ACTUATORS B-CHEMICAL,2008,129(1):146-151.
APA Chen, Zhao-Peng,Peng, Zhao-Feng,Jiang, Jian-Hui,Zhang, Xiao-Bing,Shen, Guo-Li,&Yu, Ru-Qin.(2008).An electrochemical amplification immunoassay using biocatalytic metal deposition coupled with anodic stripping voltammetric detection.SENSORS AND ACTUATORS B-CHEMICAL,129(1),146-151.
MLA Chen, Zhao-Peng,et al."An electrochemical amplification immunoassay using biocatalytic metal deposition coupled with anodic stripping voltammetric detection".SENSORS AND ACTUATORS B-CHEMICAL 129.1(2008):146-151.
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