Two Foreign Antimicrobial Peptides Expressed in the Chloroplast of Porphyridium purpureum Possessed Antibacterial Properties

doi:10.3390/md20080484

	Two Foreign Antimicrobial Peptides Expressed in the Chloroplast of Porphyridium purpureum Possessed Antibacterial Properties
	Han, Subing 1,2; Zhao, Jialin 3; Liu, Ping 1; Wang, Kang 2; Qin, Song2 ; Zhao, Zhenjun 1; Cui, Yulin2
发表期刊	MARINE DRUGS
	2022-08-01
卷号	20 期号:8 页码:11
关键词	Porphyridium purpureum chloroplast transformation system microparticle bombardment antimicrobial peptides NZ2114 Piscidin-4 inhibitory effect
DOI	10.3390/md20080484
通讯作者	Cui, Yulin(yulincui@yic.ac.cn)
英文摘要	To solve the problem of antibiotic abuse in aquaculture and to utilize the application potential of antimicrobial peptides (AMPs), a chloroplast transformation system of Porphyridium purpureum was successfully constructed for effectively expressing two exogenous AMPs. The endogenous fragments of 16S rDNA/trnA-23S rDNA were used as flanking fragments for the homologous recombination in the chloroplast genome. Two AMPs encoded by the transformation vector were controlled by the native promoter psbB in a polycistron. The plasmids were transferred into P. purpureum via particle bombardment and the transformation vectors were screened using phosphinothricin (bar), a dominant selection marker under the control of the psbA promoter. Subsequently, in the positive transformed colonies, the exogenous fragments were found to be inserted in the flanking fragments directionally as expected and two foreign AMPs were successfully obtained. Finally, two exogenous peptides with antibacterial properties were obtained from the transformed strain. The two AMPs expressed by the transformed strain were shown to have similar inhibitory effects to antibiotics by inhibition tests. This suggested that AMPs can be introduced into aquaculture using baited microalgae, providing new ideas and ways to solve a series of aquaculture diseases caused by bacteria.
资助机构	National Natural Science Foundation of China ; Project of Innovation and Development of Marine Economy
收录类别	SCI
语种	英语
关键词[WOS]	TETRASELMIS SUBCORDIFORMIS PRASINOPHYCEAE ; PLECTASIN ; NZ2114 ; TRANSFORMATION ; CHLOROPHYTA ; VACCINES ; PROTEIN
研究领域[WOS]	Pharmacology & Pharmacy
WOS记录号	WOS:000846719300001
引用统计	被引频次：2[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/31665
专题	海岸带生物学与生物资源利用重点实验室海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
通讯作者	Cui, Yulin
作者单位	1.Yantai Univ, Coll Life Sci, Yantai 264000, Peoples R China 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Biol Resource Utilizat, Yantai 264003, Peoples R China 3.Yantai Marine Econ Res Inst, Yantai 264003, Peoples R China
推荐引用方式 GB/T 7714	Han, Subing,Zhao, Jialin,Liu, Ping,et al. Two Foreign Antimicrobial Peptides Expressed in the Chloroplast of Porphyridium purpureum Possessed Antibacterial Properties[J]. MARINE DRUGS,2022,20(8):11.
APA	Han, Subing.,Zhao, Jialin.,Liu, Ping.,Wang, Kang.,Qin, Song.,...&Cui, Yulin.(2022).Two Foreign Antimicrobial Peptides Expressed in the Chloroplast of Porphyridium purpureum Possessed Antibacterial Properties.MARINE DRUGS,20(8),11.
MLA	Han, Subing,et al."Two Foreign Antimicrobial Peptides Expressed in the Chloroplast of Porphyridium purpureum Possessed Antibacterial Properties".MARINE DRUGS 20.8(2022):11.