Molecular fluorescent probes for imaging and evaluation of peroxynitrite fluctuations in living cells and in vivo under hypoxic stress

doi:10.1016/j.snb.2022.132410

	Molecular fluorescent probes for imaging and evaluation of peroxynitrite fluctuations in living cells and in vivo under hypoxic stress
	Huang, Yan 1; Yu, Lei 2; Fu, Lili 1; Hou, Junjun 4; Wang, Lingxiao 1; Sun, Mingzhao 1; Wang, Xiaoyan 1,3; Chen, Lingxin1,3,5
发表期刊	SENSORS AND ACTUATORS B-CHEMICAL
	2022-11-01
卷号	370 页码:7
关键词	Fluorescent probe Peroxynitrite Hypoxia stress Near infrared Fluorescence imaging
DOI	10.1016/j.snb.2022.132410
通讯作者	Chen, Lingxin(lxchen@yic.ac.cn)
英文摘要	Hypoxia stress causes cell damage and apoptosis by producing excessive reactive oxygen species (ROS), which is a universal concern in biology and medicine. Peroxynitrite (ONOO-) plays a vital part in the oxidative injury of tissue proteins in the pathogenic mechanism of many disorders, which is a one of ROS. Thus, excessive pro-duction of ONOO- may be one of the crucial factors leading to hypoxia stress injury. However, due to the unstable chemical property and strong reactivity of ONOO-, most of the existing detection ways cannot image and detect its level in real time. Herein, in order to selectively imaging and detecting ONOO- in living cells and in vivo, we synthesized a fluorescent probe Cy-CF3 (our fluorescent probe for detection of ONOO-). Cy-CF3 was capable of monitoring ONOO- with excellent sensitivity and selectivity. Using Cy-CF3, the relationship between hypoxia stress and ONOO- was evaluated. The probe was also employed to sense the change of ONOO- in zebrafish under hypoxia conditions as well as determine ONOO- in living mice with acute liver ischemia. Taken together, the proposed probe maybe a powerful chemical tool for the study of ONOO- in biological mechanisms.
资助机构	National Nature Science Foundation of China ; science and technology innovation development plan of Yantai of China ; Research Initiation Fund of Binzhou Medical University ; Taishan Scholar Project Special Funding ; Shandong Peninsula Engineering Research Center of Comprehensive Brine Utilization ; Weifang Science and technology development project
收录类别	SCI
语种	英语
关键词[WOS]	REACTIVE OXYGEN ; MITOCHONDRIA ; 2-PHOTON ; CONSTRUCTION ; DISEASE ; DNA
研究领域[WOS]	Chemistry ; Electrochemistry ; Instruments & Instrumentation
WOS记录号	WOS:000841960100004
引用统计	被引频次：10[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/31632
专题	中国科学院海岸带环境过程与生态修复重点实验室中国科学院海岸带环境过程与生态修复重点实验室_海岸带环境工程技术研究与发展中心
通讯作者	Chen, Lingxin
作者单位	1.Binzhou Med Univ, Sch Pharm, Yantai 264003, Peoples R China 2.Weifang Univ Sci & Technol, Shandong Peninsula Engn Res Ctr Comprehens Brine U, Weifang 262700, Peoples R China 3.Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Shandong Key Lab Coastal Environm Proc,YICCAS, Yantai 264003, Peoples R China 4.Binzhou Med Univ Hosp, Dept Resp Med, Binzhou 256603, Peoples R China 5.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Huang, Yan,Yu, Lei,Fu, Lili,et al. Molecular fluorescent probes for imaging and evaluation of peroxynitrite fluctuations in living cells and in vivo under hypoxic stress[J]. SENSORS AND ACTUATORS B-CHEMICAL,2022,370:7.
APA	Huang, Yan.,Yu, Lei.,Fu, Lili.,Hou, Junjun.,Wang, Lingxiao.,...&Chen, Lingxin.(2022).Molecular fluorescent probes for imaging and evaluation of peroxynitrite fluctuations in living cells and in vivo under hypoxic stress.SENSORS AND ACTUATORS B-CHEMICAL,370,7.
MLA	Huang, Yan,et al."Molecular fluorescent probes for imaging and evaluation of peroxynitrite fluctuations in living cells and in vivo under hypoxic stress".SENSORS AND ACTUATORS B-CHEMICAL 370(2022):7.