Effects of the potential probiotic Bacillus subtilis D1-2 on growth, digestion, immunity and intestinal flora in juvenile sea cucumber, Apostichopus japonicus

doi:10.1016/j.fsi.2022.03.043

	Effects of the potential probiotic Bacillus subtilis D1-2 on growth, digestion, immunity and intestinal flora in juvenile sea cucumber, Apostichopus japonicus
	Wang, Mengmei 1; Lv, Chengjie 2,3; Chen, Yuying 2,3; Bi, Xiujuan 2,3; Yang, Dinglong2,3 ; Zhao, Jianmin2,3,4
发表期刊	FISH & SHELLFISH IMMUNOLOGY
ISSN	1050-4648
	2022-05-01
卷号	124 页码:12-20
关键词	Apostichopus japonicus Bacillus subtilis Growth performance Digestive enzyme Immunity Intestinal flora
DOI	10.1016/j.fsi.2022.03.043
英文摘要	In the present study, a potential probiotic Bacillus subtilis D1-2 with antibacterial activity was isolated from the gut of Apostichopus japonicus. The purpose of this experiment was to assess the effect of B. subtilis D1-2 at different concentrations (C: 0 CFU/g, BL: 10(5) CFU/g, BM: 10(7) CFU/g and BH: 10(9) CFU/g) on the growth performance, digestive enzyme activity, immune ability and intestinal flora of A. japonicus. After the 56-day feeding trial, the final body weight and weight gain rate of juvenile sea cucumber A. japonicus fed B. subtilis D1-2 were significantly increased, especially in the BM group. Additionally, the lipase activity of the intestine was significantly increased in the BM and BH groups. Enhanced immunity was also found in sea cucumbers supplemented with B. subtilis D1-2. Alpha diversity indices showed that the B. subtilis D1-2-supplemented groups had higher intestinal microbial richness and diversity than the control group. The beta diversity analysis indicated that the bacterial communities in the B. subtilis D1-2-supplemented groups were quite similar but different from the bacterial communities in the control group. Dietary supplementation with B. subtilis D1-2 increased the relative abundance of some potential probiotic-related genera, including Lactobacillus, Clostridium, Lactococcus, Bifidobacterium and Streptococcus. In conclusion, dietary addition of B. subtilis D1-2 could effectively promote the growth of A. japonicus, improve its digestion and immunity capacity to a certain extent, and actively regulate the intestinal microflora of A. japonicus.
收录类别	SCI
语种	英语
关键词[WOS]	MICROBIOTA ; SUPPLEMENTATION ; PARAMETERS ; RESISTANCE ; EXPRESSION ; GENES
研究领域[WOS]	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS记录号	WOS:000793042900002
引用统计	被引频次：12[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/31060
专题	海岸带生物学与生物资源利用重点实验室支撑部门_中国科学院牟平海岸带环境综合试验站海岸带生物学与生物资源利用重点实验室_海岸带生物资源高效利用研究与发展中心
通讯作者	Yang, Dinglong
作者单位	1.Ningbo Univ, Sch Marine Sci, Ningbo 315211, Peoples R China 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China 3.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Muping Coastal Environm Res Stn, Yantai 264003, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Shandong, Peoples R China
推荐引用方式 GB/T 7714	Wang, Mengmei,Lv, Chengjie,Chen, Yuying,et al. Effects of the potential probiotic Bacillus subtilis D1-2 on growth, digestion, immunity and intestinal flora in juvenile sea cucumber, Apostichopus japonicus[J]. FISH & SHELLFISH IMMUNOLOGY,2022,124:12-20.
APA	Wang, Mengmei,Lv, Chengjie,Chen, Yuying,Bi, Xiujuan,Yang, Dinglong,&Zhao, Jianmin.(2022).Effects of the potential probiotic Bacillus subtilis D1-2 on growth, digestion, immunity and intestinal flora in juvenile sea cucumber, Apostichopus japonicus.FISH & SHELLFISH IMMUNOLOGY,124,12-20.
MLA	Wang, Mengmei,et al."Effects of the potential probiotic Bacillus subtilis D1-2 on growth, digestion, immunity and intestinal flora in juvenile sea cucumber, Apostichopus japonicus".FISH & SHELLFISH IMMUNOLOGY 124(2022):12-20.