UPLC-TOF-MS Method for Simultaneous Quantification of Steroid Hormones in Tissue Homogenates of Zebrafish with Solid-Phase Extraction

doi:10.3390/molecules26206213

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	UPLC-TOF-MS Method for Simultaneous Quantification of Steroid Hormones in Tissue Homogenates of Zebrafish with Solid-Phase Extraction
	Li, Yaxi 1,2; Yan, Zhi 1,3; Li, Xiaodong 1; Yin, Xiuli1 ; Li, Ke1,4
发表期刊	MOLECULES
	2021-10-01
卷号	26 期号:20 页码:13
关键词	quantitative analysis validation steroid hormone stressor behavioral ecology
DOI	10.3390/molecules26206213
通讯作者	Li, Ke(kli@yic.ac.cn)
英文摘要	The quantification of steroid hormones of individual zebrafish (Danio rerio) provides perspective to understand endogenous hormone function. A UPLC-TOF-MS method was developed to provide a reproducible, sensitive, and efficient assay to determine the concentration of steroid hormones, including cortisol, testosterone, androstenedione, 11-deoxycortisol, 11-deoxycorticosterone, and 17-hydroxyprogesterone in whole-body homogenates of each zebrafish. Solid-phase extraction was used to sample matrix clean-up and acquired a recovery from 89.7% to 107.9%. The analytes were separated on an Aquity BEH C18 column using gradient elution. Mass spectrometric analysis was performed by single reaction monitoring (SRM) using positive electrospray ionization mode. The total running time was 6 min, which was greatly shortened compared with a previously reported method. The developed method exhibited excellent linearity for all the analytes, with regression coefficients higher than 0.99. The limit of detection varied between 0.1 and 0.5 ng/L and the limit of quantification was 0.5-1.7 ng/L for all analytes. The precision of the method was assessed on replicate measurements and was found to be in the ranges of 1.9 % to 6.6% and 4.3% to 8.6%, for intra- and inter-day analysis, respectively. This method was validated according to FDA guidance and applied to determine steroid hormone levels in the tissue homogenate of zebrafish acutely treated with caffeine and ethanol.
资助机构	Key Deployment Project of the Center for Ocean Mega-Science, Chinese Academy of Sciences ; STS Program of CAS ; National Overseas High-level Talent Project ; Taishan Scholar Program from Shandong Province of China ; Shuangbai Plan from Yantai Municipal City
收录类别	SCI
语种	英语
关键词[WOS]	TANDEM MASS-SPECTROMETRY ; LC-MS/MS ; SALIVARY CORTISOL ; ADULT ZEBRAFISH ; STRESS ; IMMUNOASSAY ; TESTOSTERONE ; GLUCURONIDES ; SENSITIVITY ; RESOLUTION
研究领域[WOS]	Biochemistry & Molecular Biology ; Chemistry
WOS记录号	WOS:000716404400001
引用统计	被引频次：8[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/30736
专题	海岸带生物学与生物资源利用重点实验室_海岸带生物资源高效利用研究与发展中心支撑部门_所级公共技术服务中心
通讯作者	Li, Ke
作者单位	1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China 2.Univ Chinese Acad Sci, Coll Resources & Environm, Beijing 100049, Peoples R China 3.Yantai Univ, Sch Ocean, Yantai 264005, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Li, Yaxi,Yan, Zhi,Li, Xiaodong,et al. UPLC-TOF-MS Method for Simultaneous Quantification of Steroid Hormones in Tissue Homogenates of Zebrafish with Solid-Phase Extraction[J]. MOLECULES,2021,26(20):13.
APA	Li, Yaxi,Yan, Zhi,Li, Xiaodong,Yin, Xiuli,&Li, Ke.(2021).UPLC-TOF-MS Method for Simultaneous Quantification of Steroid Hormones in Tissue Homogenates of Zebrafish with Solid-Phase Extraction.MOLECULES,26(20),13.
MLA	Li, Yaxi,et al."UPLC-TOF-MS Method for Simultaneous Quantification of Steroid Hormones in Tissue Homogenates of Zebrafish with Solid-Phase Extraction".MOLECULES 26.20(2021):13.