YIC-IR  > 中科院海岸带环境过程与生态修复重点实验室
Differentially expressed genes related to oxidoreductase activity and glutathione metabolism underlying the adaptation of Phragmites australis from the salt marsh in the Yellow River Delta, China
Zhang, Liwen1,2; Chen, Lin1,2; Lan, Siqun1,2; Han, Guangxuan1,2; Chen, Lin3; Liu, Ziting3; Lu, Feng4; Lan, Siqun5
Source PublicationPEERJ
ISSN2167-8359
2020-10-02
Volume8Pages:e10024
KeywordTranscriptome analysis Salt stress Glutathione metabolism Phragmites australis Yellow River Delta Oxidoreductase activity Common reed Coastal wetland Transcription factors Differential gene expression
DOI10.7717/peerj.10024
AbstractThe common reed (Phragmites australis) is a dominant species in the coastal wetlands of the Chinese Yellow River Delta, where it tolerates a wide range of salinity. Recent environmental changes have led to the increase of soil salinity in this region, which has degraded much of the local vegetation. Clones of common reeds from the tidal marsh may have adapted to local high salinity habitat through selection on genes and metabolic pathways conferring salt tolerance. This study aims to reveal molecular mechanisms underlying salt tolerance in the tidal reed by comparing them to the salt-sensitive freshwater reed under salt stress. We employed comparative transcriptomics to reveal the differentially expressed genes (DEGs) between these two types of common reeds under different salinity conditions. The results showed that only three co-expressed genes were up-regulated and one co-expressed gene was down-regulated between the two reed types. On the other hand, 1,371 DEGs were exclusively up-regulated and 285 DEGs were exclusively down-regulated in the tidal reed compared to the control, while 115 DEGs were exclusively up-regulated and 118 DEGs were exclusively down-regulated in the freshwater reed compared to the control. From the pattern of enrichment of transcripts involved in salinity response, the tidal reed was more active and efficient in scavenging reactive oxygen species (ROS) than the freshwater reed, with the tidal reed showing significantly higher gene expression in oxidoreductase activity. Furthermore, when the reeds were exposed to salt stress, transcripts encoding glutathione metabolism were up-regulated in the tidal reed but not in the freshwater reed. DEGs related to encoding glutathione reductase (GR), glucose-6-phosphate 1-dehydrogenase (G6PDH), 6-phosphogluconate dehydrogenase (6PD), glutathione S-transferase (GST) and L-ascorbate peroxidase (LAP) were revealed as especially highly differentially regulated and therefore represented candidate genes that could be cloned into plants to improve salt tolerance. Overall, more genes were up-regulated in the tidal reed than in the freshwater reed from the Yellow River Delta when under salt stress. The tidal reed efficiently resisted salt stress by up-regulating genes encoding for oxidoreductase activity and glutathione metabolism. We suggest that this type of common reed could be extremely useful in the ecological restoration of degraded, high salinity coastal wetlands in priority.
Funding OrganizationNational Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31670533] ; Youth Innovation Promotion Association CAS [2018247] ; Science and Technology Service Network Initiative [KFJ-STS-ZDTP-023] ; key Deployment Project of Chinese Academy of Sciences [KFZD-SW-112]
Indexed BySCI
Language英语
WOS KeywordTRANSCRIPTION FACTORS ; SALINITY STRESS ; TOLERANCE ; ARABIDOPSIS ; RESPONSES ; REDUCTASE ; WETLAND ; DIVERSITY ; GENOTYPES ; NETWORK
WOS Research AreaScience & Technology - Other Topics
WOS IDWOS:000574775800002
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.yic.ac.cn/handle/133337/30498
Collection中科院海岸带环境过程与生态修复重点实验室
Affiliation1.Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Beijing, Peoples R China;
2.YICCAS, Shandong Key Lab Coastal Environm Proc, Yantai, Peoples R China;
3.Liaocheng Univ, Coll Environm & Planning, Liaocheng, Shandong, Peoples R China;
4.Adm Comm Shandong Yellow River Delta Natl Nat Res, Dongying, Peoples R China;
5.Ludong Univ, Sch Resources & Environm Engn, Yantai, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Liwen,Chen, Lin,Lan, Siqun,et al. Differentially expressed genes related to oxidoreductase activity and glutathione metabolism underlying the adaptation of Phragmites australis from the salt marsh in the Yellow River Delta, China[J]. PEERJ,2020,8:e10024.
APA Zhang, Liwen.,Chen, Lin.,Lan, Siqun.,Han, Guangxuan.,Chen, Lin.,...&Lan, Siqun.(2020).Differentially expressed genes related to oxidoreductase activity and glutathione metabolism underlying the adaptation of Phragmites australis from the salt marsh in the Yellow River Delta, China.PEERJ,8,e10024.
MLA Zhang, Liwen,et al."Differentially expressed genes related to oxidoreductase activity and glutathione metabolism underlying the adaptation of Phragmites australis from the salt marsh in the Yellow River Delta, China".PEERJ 8(2020):e10024.
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