| Enhanced biodegradation of crude oil by constructed bacterial consortium comprising salt-tolerant petroleum degraders and biosurfactant producers | |
Chen, Weiwei1,2,3; Kong, Yachao1,2,3; Li, Junde1,2,3; Sun, Yanyu1,2,3; Min, Jun1,3 ; Hu, Xiaoke1,3,4; Min, Jun4
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| 发表期刊 | INTERNATIONAL BIODETERIORATION & BIODEGRADATION
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| ISSN | 0964-8305 |
| 2020-10 | |
| 卷号 | 154页码:105047 |
| 关键词 | Crude oil Consortium Biosurfactant Bioaugmentation Alkane hydroxylase genes |
| DOI | 10.1016/j.ibiod.2020.105047 |
| 英文摘要 | Bioremediation is an attractive strategy of utilizing bacteria to remove crude oil contaminants. In this study, two salt-tolerant crude oil-degrading and biosurfactant-producing bacteria, Dietzia sp. CN-3 and Acinetobacter sp. HC8-3S, were functionally combined to construct a bacterial consortium. The consortium achieved 95.8% degradation efficiency of crude oil in 10 days and various n-alkanes, cycloalkanes, branched alkanes and aromatic hydrocarbons were all depleted more effectively than single strains. Functional optimization of the consortium degraded crude oil efficiently in a wide range of pH (4-10) and salinity (0-120 g L-1). Furthermore, two alkane hydroxylase genes, alkB in CN-3 and alkM in HC8-3S, were cloned and their expression were examined by real-time quantitative polymerase chain reaction, indicating that alkB was more prominent in long-chain alkanes (C-20, C-24 and C-26) utilization and alkM played crucial roles in medium- and long-chain alkanes (C-14, C-16, C-20, C-24 and C-26) degradation. In soil microcosms artificially contaminated with crude oil and bioaugmented with the consortium, 58.3% of total petroleum hydrocarbons were depleted after 60 days and the degradation rate (485.8 mg kg(-1) d(-1)) was higher than those reported in previous studies. Consequently, the consortium is a promising candidate in crude oil bioremediation. |
| 资助机构 | Ministry of Science and Technology of the People's Republic of ChinaMinistry of Science and Technology, China [2016YFC1402300] ; Youth Innovation Promotion Association of the Chinese Academy of Sciences, China [2020218] ; Yantai Science and Technology Bureau, China [2017ZH092] ; Chinese Academy of Sciences, ChinaChinese Academy of Sciences [133337KYSB20180015] |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | CONTAMINATED SOIL ; GENE-EXPRESSION ; DEGRADATION ; BIOREMEDIATION ; BIOAUGMENTATION ; HYDROCARBONS ; STRAINS ; EFFICIENCY ; COASTAL ; ALKANES |
| 研究领域[WOS] | Biotechnology & Applied Microbiology ; Environmental Sciences & Ecology |
| WOS记录号 | WOS:000576666100002 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/30496 |
| 专题 | 海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室 |
| 作者单位 | 1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Bioresource Utilizat, Chunhui Rd, Yantai 264003, Peoples R China; 2.Univ Chinese Acad Sci, Beijing, Peoples R China; 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China; 4.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Weiwei,Kong, Yachao,Li, Junde,et al. Enhanced biodegradation of crude oil by constructed bacterial consortium comprising salt-tolerant petroleum degraders and biosurfactant producers[J]. INTERNATIONAL BIODETERIORATION & BIODEGRADATION,2020,154:105047. |
| APA | Chen, Weiwei.,Kong, Yachao.,Li, Junde.,Sun, Yanyu.,Min, Jun.,...&Min, Jun.(2020).Enhanced biodegradation of crude oil by constructed bacterial consortium comprising salt-tolerant petroleum degraders and biosurfactant producers.INTERNATIONAL BIODETERIORATION & BIODEGRADATION,154,105047. |
| MLA | Chen, Weiwei,et al."Enhanced biodegradation of crude oil by constructed bacterial consortium comprising salt-tolerant petroleum degraders and biosurfactant producers".INTERNATIONAL BIODETERIORATION & BIODEGRADATION 154(2020):105047. |
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| Enhanced biodegradat（7834KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | 浏览 请求全文 | |
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