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14-3-3 zeta and 14-3-3 epsilon are involved in innate immune responses in Pacific abalone (Haliotis discus hannai)
Xue, Rui1,2,3; Yang, Dinglong1,2,3; Han, Yijing1,4; Deng, Qinyou5; Wang, Xin1,4; Liu, Xiangquan5; Zhao, Jianmin1,2,3
Keyword14-3-3 zeta 14-3-3 epsilon Immune responses Pacific abalone
Corresponding AuthorYang, Dinglong(dlyang@yic.ac.cn) ; Zhao, Jianmin(jmzhao@yic.ac.cn)
AbstractThe 14-3-3 proteins play important roles in various cellular processes by binding to different ligands, but little is known about these proteins in mollusks. In this study, two 14-3-3 cDNAs were identified from the Pacific abalone Haliotis discus hannai (designated 14-3-3 zeta and 14-3-3 epsilon), possessing 59.40% identity with each other. Both genes were predominantly expressed in the gills of unchallenged abalones, and their mRNA signals could also be detected in several other tissues, including the mantle, hepatopancreas and ovary. However, after Vibrio harveyi challenge, hemocytes were induced significantly (p < 0.01). Meanwhile, phagocytosis was inhibited, but apoptosis, reactive oxygen species formation, and caspase 3 expression were significantly induced (p < 0.01), and they were all suppressed with 14-3-3 zeta knockdown (p < 0.01). The differences were that silencing 14-3-3 epsilon reverted the decline in the phagocytic rate derived from bacterial infection, while ROS formation was not influenced significantly. In addition, the expression levels of several antimicrobial peptide and proinflammatory cytokine genes were also decreased with the silencing of 14-3-3 genes. However, with the knockdown of 14-3-3 zeta, the expression of 14-3-3 epsilon was further significantly increased (p < 0.01), and vice versa. Overall, our results suggested that 14-3-3 zeta and 14-3-3 epsilon should play important roles in innate immunity against V. harveyi infection.
Funding OrganizationStrategic Priority Research Program of the Chinese Academy of Sciences ; National Natural Science Foundation of China ; Natural Science Foundation of Shandong Province ; Yantai Science and Technology Development Project ; Youth Innovation Promotion Association of CAS
Indexed BySCI
WOS Research AreaFisheries ; Immunology ; Veterinary Sciences ; Zoology
WOS IDWOS:000692706800002
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Document Type期刊论文
Corresponding AuthorYang, Dinglong; Zhao, Jianmin
Affiliation1.Yantai Inst Coastal Zone Res, Muping Coastal Environm Res Stn, Yantai 264117, Shandong, Peoples R China
2.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Shandong, Peoples R China
3.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res & Dev Ctr Efficient Utilizat Coastal Bioresou, Yantai 264003, Shandong, Peoples R China
4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
5.Shandong Marine Resource & Environm Res Inst, Yantai 264006, Peoples R China
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Xue, Rui,Yang, Dinglong,Han, Yijing,et al. 14-3-3 zeta and 14-3-3 epsilon are involved in innate immune responses in Pacific abalone (Haliotis discus hannai)[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2021,124:12.
APA Xue, Rui.,Yang, Dinglong.,Han, Yijing.,Deng, Qinyou.,Wang, Xin.,...&Zhao, Jianmin.(2021).14-3-3 zeta and 14-3-3 epsilon are involved in innate immune responses in Pacific abalone (Haliotis discus hannai).DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,124,12.
MLA Xue, Rui,et al."14-3-3 zeta and 14-3-3 epsilon are involved in innate immune responses in Pacific abalone (Haliotis discus hannai)".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 124(2021):12.
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