| Identification and functional analysis of a sex-biased transcriptional factor Foxl2 in the bay scallop Argopecten irradians irradians | |
Ning, Junhao1; Cao, Weian2; Lu, Xia1 ; Chen, Min1; Liu, Bo2; Wang, Chunde1,2
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| 发表期刊 | COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
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| ISSN | 1096-4959 |
| 2021-10-01 | |
| 卷号 | 256页码:8 |
| 关键词 | Argopecten irradians irradians Foxl2 Expression analysis RNA interference |
| DOI | 10.1016/j.cbpb.2021.110638 |
| 通讯作者 | Wang, Chunde(chundewang2007@163.com) |
| 英文摘要 | Transcription factor Foxl2 is an evolutionarily conserved gene playing pivotal roles in regulation of early ovarian differentiation and maintenance in animals. However, the Foxl2 gene has not been thoroughly studied in hermaphroditic scallops. In this study, we cloned and characterized a Foxl2 (designated as AiFoxl2) from the bay scallop Argopecten irradians irradians. The open reading frame of AiFoxl2 was 1122 bp encoding 373 amino acids residues and contained a conserved forkhead box domain. Quantitative real-time PCR showed that AiFoxl2 was mainly expressed in the ovary. Moreover, the highest expression of AiFoxl2 in the ovary was detected at proliferative stage and growing stage, while the lowest level was found at resting stage. During the embryonic and larval development, expression of AiFoxl2 was found first in fertilized eggs, increased significantly at the blastula stage, and reached peak value at the D-larvae stage. When AiFoxl2 was knocked down, testis development-related genes (Dmrt1, Sox7 and Sox9) were up-regulated significantly while the ovary development-related genes (Vg, HSD14, and GATA-1) were down-regulated manifestly. These findings suggested that AiFoxl2 was a femalerelated gene in A. i. irradians and may be involved in regulation of ovarian development and differentiation. |
| 资助机构 | National Natural Science Foundation of China ; Scientific and Technological Project of Yantai, Shandong Province ; Key Deployment Projects of Center for Ocean Mega-Science, Chinese Academy of Sciences |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | MOLECULAR-CLONING ; GENE-EXPRESSION ; DMRT1 ; DIFFERENTIATION ; MAINTENANCE ; TILAPIA ; TRAITS ; CELLS |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Zoology |
| WOS记录号 | WOS:000684852500021 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/29656 |
| 专题 | 海岸带生物学与生物资源利用重点实验室_海岸带生物资源高效利用研究与发展中心 中国科学院烟台海岸带研究所 |
| 通讯作者 | Wang, Chunde |
| 作者单位 | 1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China 2.Qingdao Agr Univ, Coll Marine Sci & Engn, Qingdao 266109, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ning, Junhao,Cao, Weian,Lu, Xia,et al. Identification and functional analysis of a sex-biased transcriptional factor Foxl2 in the bay scallop Argopecten irradians irradians[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,2021,256:8. |
| APA | Ning, Junhao,Cao, Weian,Lu, Xia,Chen, Min,Liu, Bo,&Wang, Chunde.(2021).Identification and functional analysis of a sex-biased transcriptional factor Foxl2 in the bay scallop Argopecten irradians irradians.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,256,8. |
| MLA | Ning, Junhao,et al."Identification and functional analysis of a sex-biased transcriptional factor Foxl2 in the bay scallop Argopecten irradians irradians".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY 256(2021):8. |
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