Institutional Repository of Key Laboratory of Coastal Zone Environmental Processes, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences (KLCEP)
| Three dimensionally printed nitrocellulose-based microfluidic platform for investigating the effect of oxygen gradient on cells | |
Liu, Ping1,2; Fu, Longwen2,3 ; Song, Zhihua4; Man, Mingsan2; Yuan, Huamao3,5; Zheng, Xiaoli1; Kang, Qi1; Shen, Dazhong1; Song, Jinming3; Li, Bowei2,3; Chen, Lingxin2,3
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| Source Publication | ANALYST
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| ISSN | 0003-2654 |
| 2021-07-29 | |
| Pages | 9 |
| DOI | 10.1039/d1an00927c |
| Corresponding Author | Shen, Dazhong(dzshen@sdnu.edu.cn) ; Li, Bowei(bwli@yic.ac.cn) ; Chen, Lingxin(lxchen@yic.ac.cn) |
| Abstract | In this article, we present a novel nitrocellulose-based microfluidic chip with 3-dimensional (3D) printing technology to study the effect of oxygen gradient on cells. Compared with conventional polydimethylsiloxane (PDMS) chips of oxygen gradient for cell cultures that can only rely on fluorescence microscope analysis, this hybrid nitrocellulose-based microfluidic platform can provide a variety of analysis methods for cells, including flow cytometry, western blot and RT-PCR, because the nitrocellulose-based chips with cells can be taken out from the growth chambers of 3D printed microfluidic chip and then used for cell collection or lysis. These advantages allow researchers to acquire more information and data on the basic biochemical and physiological processes of cell life. The effect of oxygen gradient on the zebrafish cells (ZF4) was used as a model to show the performance and application of our platform. Hypoxia caused the increase of intercellular reactive oxygen species (ROS) and accumulation of hypoxia-inducible factor 1 alpha (HIF-1 alpha). Hypoxia stimulated the transcription of hypoxia-responsive genes vascular endothelial growth factor (VEGF) and induced cell cycle arrest of ZF4 cells. The established platform is able to obtain more information from cells in response to different oxygen concentration, which has potential for analyzing the cells under a variety of pathological conditions. |
| Funding Organization | National Natural Science Foundation of China ; National Key Research and Development Program of China ; Key Deployment Project of Centre for Ocean Mega-Research of Science, Chinese Academy of Sciences ; Open Fund of CAS Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences |
| Indexed By | SCI |
| Language | 英语 |
| WOS Keyword | GROWTH-FACTOR VEGF ; FLUORESCENCE DETECTION ; CANCER-CELLS ; HYPOXIA ; GENERATION ; ZEBRAFISH ; CULTURE ; DEVICE ; CHIP ; ANGIOGENESIS |
| WOS Research Area | Chemistry |
| WOS ID | WOS:000678901200001 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.yic.ac.cn/handle/133337/29558 |
| Collection | 中科院海岸带环境过程与生态修复重点实验室 中科院海岸带环境过程与生态修复重点实验室_海岸带环境工程技术研究与发展中心 |
| Corresponding Author | Shen, Dazhong; Li, Bowei; Chen, Lingxin |
| Affiliation | 1.Shandong Normal Univ, Minist Educ, Key Lab Mol & Nano Probes, Coll Chem,Chem Engn & Mat Sci, Jinan 250014, Peoples R China 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res Ctr Coastal Environm Engn & Technol, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China 4.Yantai Univ, Minist Educ, Sch Pharm, Key Lab Mol Pharmacol & Drug Evaluat, Yantai 264005, Peoples R China 5.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Ecol & Environm Sci, Qingdao, Peoples R China |
| Recommended Citation GB/T 7714 | Liu, Ping,Fu, Longwen,Song, Zhihua,et al. Three dimensionally printed nitrocellulose-based microfluidic platform for investigating the effect of oxygen gradient on cells[J]. ANALYST,2021:9. |
| APA | Liu, Ping.,Fu, Longwen.,Song, Zhihua.,Man, Mingsan.,Yuan, Huamao.,...&Chen, Lingxin.(2021).Three dimensionally printed nitrocellulose-based microfluidic platform for investigating the effect of oxygen gradient on cells.ANALYST,9. |
| MLA | Liu, Ping,et al."Three dimensionally printed nitrocellulose-based microfluidic platform for investigating the effect of oxygen gradient on cells".ANALYST (2021):9. |
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| Three dimensionally （1821KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View Download | |
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