Determination of chitosan content with Schiff base method and HPLC
Miao, Qin1,2,3; Mi, Yingqi1,2,3; Cui, Jingmin1,2,3; Zhang, Jingjing1,2; Tan, Wenqiang1,2; Li, Qing1,2; Guo, Zhanyong1,2,3
Source PublicationINTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
ISSN0141-8130
2021-07-01
Volume182Pages:1537-1542
KeywordDetermination of chitosan content Chitosan-Schiff base HPLC
DOI10.1016/j.ijbiomac.2021.05.121
Corresponding AuthorGuo, Zhanyong(zhanyongguo@hotmail.com)
AbstractTremendous awareness of determination of chitosan content accurately is increasing, due to it has great significance to the quality control of chitosan. In this article, two kinds of chitosan-Schiff base derivatives (BCSB and PCSB) were synthesized by the different average degrees of deacetylation (DD) of chitosan with benzaldehyde or propanal, respectively. The total mass of Schiff base derivative product was dried and obtained without washing and loss. Then, a certain amount of the prepared Schiff base compound was taken to hydrolyze into glucosamine hydrochloride (GAH) in strong hydrochloric acidic environment, whose concentration was quantified by HPLC, and the mass of GAH contained in hydrolysis solution could be calculated. Subsequently, the total quality of GAH obtained by hydrolysis of all of the Schiff base product was calculated and obtained, and then the theoretical mass of chitosan could be deduced and calculated by further converse calculation. Finally, the chitosan content was obtained by combining the sample mass used in Schiff base reaction and the theoretical mass of chitosan. This method was accurate and convenient, providing a preeminent idea and method for the determination of chitosan content. (c) 2021 Elsevier B.V. All rights reserved.
Funding OrganizationNational Key Research and Development Program of China ; Natural Science Foundation of Shandong Province of China
Indexed BySCI
Language英语
WOS KeywordLIQUID-CHROMATOGRAPHIC DETERMINATION ; ACID-HYDROLYSIS ; ANTIMICROBIAL ACTIVITY ; GLUCOSAMINE ; CHITIN ; DEACETYLATION ; STABILITY ; SAMPLES ; CRAB
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry ; Polymer Science
WOS IDWOS:000669494700003
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Document Type期刊论文
Identifierhttp://ir.yic.ac.cn/handle/133337/29531
Collection海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
海岸带生物学与生物资源利用重点实验室_海岸带生物资源高效利用研究与发展中心
Corresponding AuthorGuo, Zhanyong
Affiliation1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Bioresource Utilizat, Yantai 264003, Shandong, Peoples R China
2.Chinese Acad Sci, Ctr Ocean Megasci, 7 Nanhai Rd, Qingdao 266071, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Miao, Qin,Mi, Yingqi,Cui, Jingmin,et al. Determination of chitosan content with Schiff base method and HPLC[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2021,182:1537-1542.
APA Miao, Qin.,Mi, Yingqi.,Cui, Jingmin.,Zhang, Jingjing.,Tan, Wenqiang.,...&Guo, Zhanyong.(2021).Determination of chitosan content with Schiff base method and HPLC.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,182,1537-1542.
MLA Miao, Qin,et al."Determination of chitosan content with Schiff base method and HPLC".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 182(2021):1537-1542.
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