The engineered biphenyl dioxygenases enhanced the metabolism of dibenzofuran
Wang, Yuan1,2,3; Sun, Chengcheng2,3,5; Min, Jun2,3,4; Li, Bingjun1; Li, Junde2,3,5; Chen, Weiwei2,3,5; Kong, Yachao2,3,5; Hu, Xiaoke2,3,4
Source PublicationINTERNATIONAL BIODETERIORATION & BIODEGRADATION
ISSN0964-8305
2021-07-01
Volume161Pages:9
KeywordDibenzofuran Burkholderia xenovorans LB400 Biphenyl dioxygenase Enzyme engineering Regiospecificity Biocatalysts
DOI10.1016/j.ibiod.2021.105228
Corresponding AuthorHu, Xiaoke(xkhu@yic.ac.cn)
AbstractAs a model compound of dioxin, dibenzofuran is a persistent environmental pollutant. Several investigations have provided evidence that biphenyl dioxygenase (BPDO) from Burkholderia xenovorans LB400 could be engineered to further enhance the metabolite profile of biphenyl and polychlorinated biphenyl through lateral oxygenation. In this context, we examined the ability of the evolved BphAES283M, BphAEp4-S283M and BphAERR41S283M to transform dibenzofuran with features of co-planar and ortho-substituted biphenyls. For BphAES283M, BphAEp4-S283M and BphAERR41-S283M, the kcat/Km value toward dibenzofuran was 4.5 times, 3 times and 2.5 times higher than that of the wild-type enzyme, respectively. Meanwhile, biochemical experiments determined that the substitution Ser283Met affected the regiospecificity of product formation, and the primary metabolite produced by BphAES283M was identified as 3,4-dihydro-3,4-dihydroxy-dibenzofuran. The structural analysis revealed residue Met283 as critical to generate a flexible catalytic cavity and a productive orientation of dibenzofuran during the catalytic reaction. Collectively, this study provides the theoretical basis and technical support for the significant development of better promising biocatalysts to effectively degrade dibenzofuran and other aromatic pollutants in the environment.
Funding OrganizationNational Natural Science Foundation of China ; Key Research Project of Frontier Science of Chinese Academy of Sciences ; External Cooperation Program of Chinese Academy of Sciences ; Youth Innovation Promotion Association Program of the Chinese Academy of Sciences
Indexed BySCI
Language英语
WOS KeywordACTIVE-SITE ; NAPHTHALENE DIOXYGENASE ; BACTERIAL-DEGRADATION ; CATABOLIC PATHWAY ; P-DIOXIN ; STRAIN ; B-356 ; AMINO ; BURKHOLDERIA-XENOVORANS-LB400 ; DIBENZOTHIOPHENE
WOS Research AreaBiotechnology & Applied Microbiology ; Environmental Sciences & Ecology
WOS IDWOS:000656677300004
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Document Type期刊论文
Identifierhttp://ir.yic.ac.cn/handle/133337/29380
Collection海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
Corresponding AuthorHu, Xiaoke
Affiliation1.Yantai Univ, Ocean Sch, Yantai 264005, Peoples R China
2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Bioresource Utilizat, Chunhui Rd, Yantai 264003, Peoples R China
3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China
4.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao, Peoples R China
5.Univ Chinese Acad Sci, Beijing, Peoples R China
Recommended Citation
GB/T 7714
Wang, Yuan,Sun, Chengcheng,Min, Jun,et al. The engineered biphenyl dioxygenases enhanced the metabolism of dibenzofuran[J]. INTERNATIONAL BIODETERIORATION & BIODEGRADATION,2021,161:9.
APA Wang, Yuan.,Sun, Chengcheng.,Min, Jun.,Li, Bingjun.,Li, Junde.,...&Hu, Xiaoke.(2021).The engineered biphenyl dioxygenases enhanced the metabolism of dibenzofuran.INTERNATIONAL BIODETERIORATION & BIODEGRADATION,161,9.
MLA Wang, Yuan,et al."The engineered biphenyl dioxygenases enhanced the metabolism of dibenzofuran".INTERNATIONAL BIODETERIORATION & BIODEGRADATION 161(2021):9.
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