Dose-dependent responses of metabolism and tissue injuries in clam Ruditapes philippinarum after subchronic exposure to cadmium
Zhan, Junfei1,2,5; Wang, Shuang1,2,5; Li, Fei1,2,4; Ji, Chenglong1,2,3,4; Wu, Huifeng1,2,3,4
Source PublicationSCIENCE OF THE TOTAL ENVIRONMENT
ISSN0048-9697
2021-07-20
Volume779Pages:11
KeywordCadmium Dose-response Hormesis Metabolomics Ruditapes philippinarum Tissue injuries
DOI10.1016/j.scitotenv.2021.146479
Corresponding AuthorWu, Huifeng(hfwu@yic.ac.cn)
AbstractMarine cadmium (Cd) pollution has been globally occurring, which creates a pressing need to characterize toxicological effects and develop biomarkers for Cd. However, the dose-response relationships challenge toxicity characterization and biomarkers selection. Metabolic processes have been frequently targeted by Cd. In this work, we investigated the dose-dependent effects of Cd on metabolic endpoints in whole soft tissues as well as gill and hepatopancreas injuries in clam Ruditapes philippinarum, aiming to better understand the metabolic responses and develop biomarkers. Nuclear magnetic resonance (NMR)-based metabolomic analysis was conducted on clam whole soft tissues to identify metabolites. The enzymes and metabolites associated with tricarboxylic acid (TCA) cycle, glycolysis, and oxidative phosphorylation showed both monotonic and nonmonotonic curves with the increase of Cd dose. In details, glutamine, glucose-1-phosphate, hexokinase (HK), and citrate synthase (CS) presented monotonic decreases with the increase of Cd dose, among which glutamine and CS were preferable biomarkers to Cd exposure based on lower benchmark dose (BMD) values. The monotonic decreases of HK and CS activities suggested Cd exposure potentially disrupted glycolysis and TCA cycle via inhibiting rate-limiting enzymes. In contrast, the non-monotonic responses of succinate dehydrogenase (SDH), alanine aminotransferase (ALT), and their substrates (succinate and alanine) were approximate to Uor J-shaped curves, suggesting the adaptive strategy of metabolic responses to different degrees of Cd stress, like induction of anaerobiosis as energy compensation. Especially, the alterations of succinate and SDH presented & nbsp;typical hormetic dose-response curves. What is more, clam hepatopancreas was more sensitive to Cd than gill in terms of injury occurrence. Overall, characterization of dose-dependent effect of Cd on metabolism and tissue injuries provides a new insight into understanding the metabolic adaptation in marine clams and risk assessment of Cd pollution. (c) 2021 Elsevier B.V. All rights reserved.
Funding OrganizationNational Natural Science Foundation of China ; Young Taishan Scholars Program of Shandong Province
Indexed BySCI
Language英语
WOS KeywordVIRGINICA GMELIN BIVALVIA ; SHRIMP CRANGON-AFFINIS ; SUCCINATE-DEHYDROGENASE ; METAL POLLUTION ; EASTERN OYSTER ; MARINE BIVALVE ; COMPLEX II ; BAY ; HEXOKINASE ; SEAWATER
WOS Research AreaEnvironmental Sciences & Ecology
WOS IDWOS:000655685000012
Citation statistics
Cited Times:7[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.yic.ac.cn/handle/133337/29362
Collection中科院海岸带环境过程与生态修复重点实验室_海岸带环境过程实验室
中科院海岸带环境过程与生态修复重点实验室
Corresponding AuthorWu, Huifeng
Affiliation1.Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Shandong, Peoples R China
2.YICCAS, Shandong Key Lab Coastal Environm Proc, Yantai 264003, Shandong, Peoples R China
3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266237, Peoples R China
4.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China
5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Zhan, Junfei,Wang, Shuang,Li, Fei,et al. Dose-dependent responses of metabolism and tissue injuries in clam Ruditapes philippinarum after subchronic exposure to cadmium[J]. SCIENCE OF THE TOTAL ENVIRONMENT,2021,779:11.
APA Zhan, Junfei,Wang, Shuang,Li, Fei,Ji, Chenglong,&Wu, Huifeng.(2021).Dose-dependent responses of metabolism and tissue injuries in clam Ruditapes philippinarum after subchronic exposure to cadmium.SCIENCE OF THE TOTAL ENVIRONMENT,779,11.
MLA Zhan, Junfei,et al."Dose-dependent responses of metabolism and tissue injuries in clam Ruditapes philippinarum after subchronic exposure to cadmium".SCIENCE OF THE TOTAL ENVIRONMENT 779(2021):11.
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