Institutional Repository of Key Laboratory of Coastal Zone Environmental Processes, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences (KLCEP)
| Digital gene expression analysis in the gills of Ruditapes philippinarum after nitrite exposure | |
Lv, Jiasen1; Cao, Tengfei1; Ji, Chenglong2,3,4 ; Cong, Ming3,4; Zhao, Jianmin3,4; Wu, Huifeng2,3,4
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| 发表期刊 | ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
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| ISSN | 0147-6513 |
| 2019-11-30 | |
| 卷号 | 184页码:7 |
| 关键词 | Digital gene expression Gills Ruditapes philippinarum Nitrite |
| DOI | 10.1016/j.ecoenv.2019.109661 |
| 通讯作者 | Cong, Ming(mcong@yic.ac.cn) ; Wu, Huifeng(hfwu@yic.ac.cn) |
| 英文摘要 | Due to the overload of pollutants from highly intensive anthropic activities, nitrite accumulates in offshore seawater and has been a long-lasting pollutant to the healthy aquaculture of the mollusk. In the present study, Ruditapes philippinarum was used as the target bivalve to receive nitrite exposure at environmental concentration for 1 and 7 days. Differentially expressed genes (DEGs) were detected and analyzed by a digital gene expression (DGE) approach to describe the toxicity of nitrite on the bivalve at the gene level. In the N1 group, 185 DEGs were generated and enriched in six Gene Ontology (GO) terms, including oxidoreductase activity, heme binding, tetrapyrrole binding, iron ion binding, metal binding and cation binding. The DEGs in the N1 group were also enriched in two Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways, e.g., arachidonic acid metabolism and ovarian steroidogenesis. In the N7 group, 81 DEGs were generated without any GO enrichment but were enriched in five KEGG pathways, including protein processing in the endoplasmic reticulum, protein export, prion diseases, thyroid hormone synthesis and arachidonic acid metabolism. This suggested that nitrite exposure might cause adverse effects to the clams in several aspects, including oxidative damage, depressed immunity, and disorders in cell proliferation, hormone metabolism and tissue regeneration. Evaluation of oxidative stress indicated that nitrite exposure actually induced redox state imbalance by enhancing the contents of thiobarbituric acid reactive substances (TBARSs) and glutathione (GSH), and the activity of glutathione peroxidase (GSH-PX) but not superoxide dismutase (SOD). These results will provide valuable gene references for further study on the toxicology mechanism of bivalves under environmental nitrite stress. |
| 资助机构 | Natural Science Foundation of China ; Strategic Priority Research Program of the Chinese Academy of Sciences |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | LIPID-PEROXIDATION ; IMMUNE-RESPONSE ; TRANSFERASE ; AMMONIA ; METABOLISM ; TOXICITY ; NITRATE ; KEGG |
| 研究领域[WOS] | Environmental Sciences & Ecology ; Toxicology |
| WOS记录号 | WOS:000489085200020 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/29131 |
| 专题 | 中国科学院海岸带环境过程与生态修复重点实验室 海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室 中国科学院海岸带环境过程与生态修复重点实验室_海岸带环境过程实验室 海岸带生物学与生物资源利用重点实验室_海岸带生物资源高效利用研究与发展中心 海岸带生物学与生物资源利用重点实验室 |
| 通讯作者 | Cong, Ming; Wu, Huifeng |
| 作者单位 | 1.Yantai Univ, Biol Sch, Yantai 264005, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266237, Shandong, Peoples R China 3.Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China 4.YICCAS, Shandong Prov Key Lab Coastal Environm Proc, Yantai 264003, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lv, Jiasen,Cao, Tengfei,Ji, Chenglong,et al. Digital gene expression analysis in the gills of Ruditapes philippinarum after nitrite exposure[J]. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,2019,184:7. |
| APA | Lv, Jiasen,Cao, Tengfei,Ji, Chenglong,Cong, Ming,Zhao, Jianmin,&Wu, Huifeng.(2019).Digital gene expression analysis in the gills of Ruditapes philippinarum after nitrite exposure.ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,184,7. |
| MLA | Lv, Jiasen,et al."Digital gene expression analysis in the gills of Ruditapes philippinarum after nitrite exposure".ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY 184(2019):7. |
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