| Regulation of the activity and kinetic properties of alkaline phosphatase in eutrophic water bodies by phosphorus and heavy metals | |
Lv, Yingchun1,2 ; Zhang, Yang2,3; Song, Jiawei2,3; Xu, Gang1,2
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| 发表期刊 | DESALINATION AND WATER TREATMENT
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| ISSN | 1944-3994 |
| 2020-02-01 | |
| 卷号 | 177页码:131-138 |
| 关键词 | Alkaline phosphatase Kinetic characteristics Heavy metal Swan Lake |
| DOI | 10.5004/dwt.2020.24924 |
| 通讯作者 | Xu, Gang(gxu@yic.ac.cn) |
| 英文摘要 | Phosphatase-catalyzed hydrolysis of organic phosphorus is an important pathway for providing bioavailable phosphorus to aquatic organisms. In this paper, a eutrophic water body was used to investigate how phosphorus and heavy metal ions affect alkaline phosphatase activity (APA) and its kinetic properties. The results show that phosphate (PO43-), hexametaphosphate ((PO3)(6)(6-)), pyrophosphate (P2O74-), and beta-glycerophosphate (C3H4O6P2-) inhibit APA and the inhibition effects increased with increasing concentration at the range of 0.01-1 mmol L-1. Addition of heavy metal ions, that is, Co2+, Pb2+, and Cr6+ at concentrations of 1 mmol L-1, increased the APA 3.1-fold, 2.3-fold, and 2.7-fold, respectively. The Cu2+, Zn2+, and Ni2+ exhibited an inhibitory effect on APA in water. For 1 mmol L-1 Cu2+, the APA deceased by 37.7% relative to the control. As the concentrations of Cu2+, PO43-, (PO3)(6)(6-), and P2O74- increased, the V-max of the alkaline phosphatase (AP) remained stable and K-m increased, indicating that these ions are competitive inhibitors of AP. In addition, V-max/K-m decreased with increasing concentrations of Cu2+, PO43-, (PO3)(6)(6-), P2O74-, and C3H4O6P2-, demonstrating the decreased catalytic efficiency of the AP. In summary, phosphorus and heavy metal significantly affect conversion of organic phosphorus into bioavailable inorganic phosphorous by changing the APA in eutrophic water bodies. |
| 资助机构 | National Natural Science Foundation of China ; Key Deployment Project of the Chinese Academy of Sciences ; Science and Technology Service Network Initiative |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | DISSOLVED ORGANIC PHOSPHORUS ; ENZYMES ; IMPACT |
| 研究领域[WOS] | Engineering ; Water Resources |
| WOS记录号 | WOS:000544993600013 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/28632 |
| 专题 | 中国科学院海岸带环境过程与生态修复重点实验室_海岸带环境过程实验室 中国科学院海岸带环境过程与生态修复重点实验室 |
| 通讯作者 | Xu, Gang |
| 作者单位 | 1.Qufu Normal Univ, Coll Geog & Tourism, Rizhao 276826, Peoples R China 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, 17 Chunhui Rd, Yantai 264003, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lv, Yingchun,Zhang, Yang,Song, Jiawei,et al. Regulation of the activity and kinetic properties of alkaline phosphatase in eutrophic water bodies by phosphorus and heavy metals[J]. DESALINATION AND WATER TREATMENT,2020,177:131-138. |
| APA | Lv, Yingchun,Zhang, Yang,Song, Jiawei,&Xu, Gang.(2020).Regulation of the activity and kinetic properties of alkaline phosphatase in eutrophic water bodies by phosphorus and heavy metals.DESALINATION AND WATER TREATMENT,177,131-138. |
| MLA | Lv, Yingchun,et al."Regulation of the activity and kinetic properties of alkaline phosphatase in eutrophic water bodies by phosphorus and heavy metals".DESALINATION AND WATER TREATMENT 177(2020):131-138. |
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