| Label-free exonuclease I-assisted signal amplification colorimetric sensor for highly sensitive detection of kanamycin | |
Li, Jingwen1; Liu, Yongming1; Lin, Hao1; Chen, Yan2; Liu, Zhenbo1; Zhuang, Xuming1; Tian, Chunyuan1; Fu, Xiuli1; Chen, Lingxin3,4
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| 发表期刊 | FOOD CHEMISTRY
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| ISSN | 0308-8146 |
| 2021-06-15 | |
| 卷号 | 347页码:6 |
| 关键词 | Kanamycin Exonuclease I Colorimetric Protamine Gold nanoparticles |
| DOI | 10.1016/j.foodchem.2020.128988 |
| 通讯作者 | Fu, Xiuli(fxl1984.happy@163.com) ; Chen, Lingxin(lxchen@yic.ac.cn) |
| 英文摘要 | A label-free colorimetric method based on exonuclease I (Exo I)-assisted signal amplification with protamine as a medium was developed for analysis of kanamycin. In this study, a double-stranded DNA (dsDNA) probe was tailored by manipulating an aptamer and its complementary DNA (cDNA) ensuring detection of target with high selectivity and excellent sensitivity. Herein, protamine could not only combine with negatively charged gold nanoparticles but also interaction with polyanion DNA. Upon addition of target kanamycin, the target-aptamer complex was formed and the cDNA was released. Thus, both aptamer and cDNA could be digested by Exo I, and the captured kanamycin was liberated for triggering target recycling and signal amplification. Under optimized conditions, the proposed colorimetric method realized a low detection limit of 2.8 x 10(-14) M along with a wide linear range plus excellent selectivity. Our strategy exhibited enormous potentials for fabricate various kinds of biosensors based on target-induced aptamer configuration changes. |
| 资助机构 | National Natural Science Foundation of China ; Natural Science Foundation of Shandong Province in China ; Qingchuang Science and Technology Program of Shandong Province in China ; Taishan Scholar Project Special Funding |
| 收录类别 | SCI |
| 语种 | 英语 |
| 研究领域[WOS] | Chemistry ; Food Science & Technology ; Nutrition & Dietetics |
| WOS记录号 | WOS:000617924800006 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/27403 |
| 专题 | 中国科学院海岸带环境过程与生态修复重点实验室_海岸带环境工程技术研究与发展中心 中国科学院海岸带环境过程与生态修复重点实验室 |
| 通讯作者 | Fu, Xiuli; Chen, Lingxin |
| 作者单位 | 1.Yantai Univ, Sch Chem & Chem Engn, Yantai 264005, Peoples R China 2.Shandong Agr & Engn Univ, Coll Resources & Environm Engn, Jinan 250100, Peoples R China 3.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res Ctr Coastal Environm Engn & Technol, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China 4.Qufu Normal Univ, Coll Chem & Chem Engn, Qufu 273165, Shandong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Jingwen,Liu, Yongming,Lin, Hao,et al. Label-free exonuclease I-assisted signal amplification colorimetric sensor for highly sensitive detection of kanamycin[J]. FOOD CHEMISTRY,2021,347:6. |
| APA | Li, Jingwen.,Liu, Yongming.,Lin, Hao.,Chen, Yan.,Liu, Zhenbo.,...&Chen, Lingxin.(2021).Label-free exonuclease I-assisted signal amplification colorimetric sensor for highly sensitive detection of kanamycin.FOOD CHEMISTRY,347,6. |
| MLA | Li, Jingwen,et al."Label-free exonuclease I-assisted signal amplification colorimetric sensor for highly sensitive detection of kanamycin".FOOD CHEMISTRY 347(2021):6. |
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| Label-free exonuclea（1745KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | 浏览 下载 | |
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