Biotransformation mechanism of Vibrio diabolicus to sulfamethoxazole at transcriptional level
Wang, Qiaoning1,5; Wang, Hongdan1; Jiang, Yaru1; Lv, Min1; Wang, Xiaoyan4; Chen, Lingxin1,2,3
Source PublicationJOURNAL OF HAZARDOUS MATERIALS
ISSN0304-3894
2021-06-05
Volume411Pages:10
KeywordSulfamethoxazole Vibrio diabolicus strain L2-2 Transcriptome Biotransformation Resistance
DOI10.1016/j.jhazmat.2020.125023
Corresponding AuthorChen, Lingxin(lxchen@yic.ac.cn)
AbstractSulfamethoxazole (SMX) has attracted much attention due to its high probability of detection in the environment. Marine bacteria Vibrio diabolicus strain L2-2 has been proven to be able to transform SMX. In this study, the potential resistance and biotransformation mechanism of strain L2-2 to SMX, and key genes responses to SMX at environmental concentrations were researched. KEGG pathways were enriched by down-regulated genes including degradation of L-Leucine, L-Isoleucine, and fatty acid metabolism. Resistance mechanism could be concluded as the enhancement of membrane transport, antioxidation, response regulator, repair proteins, and ribosome protection. Biotransformation genes might involve in arylamine N-acetyltransferases (nat), cytochrome c553 (cyc-553) and acyl-CoA synthetase (acs). At the environmental concentration of SMX (0.1-10 mu g/L), nat was not be activated, which meant the acetylation of SMX might not occur in the environment; however, cyc-553 was up-regulated under SMX stress of 1 mu g/L, which indicated the hydroxylation of SMX could occur in the environment. Besides, the membrane transport and antioxidation of strain L2-2 could be activated under SMX stress of 10 mu g/L. The results provided a better understanding of resistance and biotransformation of bacteria to SMX and would support related researches about the impacts of environmental antibiotics.
Funding OrganizationNational Natural Science Foundation of China ; Taishan Scholar Project Special Funding of China
Indexed BySCI
Language英语
WOS Research AreaEngineering ; Environmental Sciences & Ecology
WOS IDWOS:000638080300006
Citation statistics
Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.yic.ac.cn/handle/133337/27227
Collection中科院海岸带环境过程与生态修复重点实验室_海岸带环境过程实验室
中科院海岸带环境过程与生态修复重点实验室
Corresponding AuthorChen, Lingxin
Affiliation1.Chinese Acad Sci, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Shandong Prov Key Lab Coastal Environm Proc, Yantai Inst Coastal Zone Res,Res Ctr Coastal Envi, Yantai 264003, Peoples R China
2.Pilot Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China
3.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China
4.Binzhou Med Univ, Sch Pharm, Yantai 264003, Peoples R China
5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Wang, Qiaoning,Wang, Hongdan,Jiang, Yaru,et al. Biotransformation mechanism of Vibrio diabolicus to sulfamethoxazole at transcriptional level[J]. JOURNAL OF HAZARDOUS MATERIALS,2021,411:10.
APA Wang, Qiaoning,Wang, Hongdan,Jiang, Yaru,Lv, Min,Wang, Xiaoyan,&Chen, Lingxin.(2021).Biotransformation mechanism of Vibrio diabolicus to sulfamethoxazole at transcriptional level.JOURNAL OF HAZARDOUS MATERIALS,411,10.
MLA Wang, Qiaoning,et al."Biotransformation mechanism of Vibrio diabolicus to sulfamethoxazole at transcriptional level".JOURNAL OF HAZARDOUS MATERIALS 411(2021):10.
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