| Biotransformation mechanism of Vibrio diabolicus to sulfamethoxazole at transcriptional level | |
Wang, Qiaoning1,5; Wang, Hongdan1; Jiang, Yaru1; Lv, Min1 ; Wang, Xiaoyan4; Chen, Lingxin1,2,3
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| Source Publication | JOURNAL OF HAZARDOUS MATERIALS
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| ISSN | 0304-3894 |
| 2021-06-05 | |
| Volume | 411Pages:10 |
| Keyword | Sulfamethoxazole Vibrio diabolicus strain L2-2 Transcriptome Biotransformation Resistance |
| DOI | 10.1016/j.jhazmat.2020.125023 |
| Corresponding Author | Chen, Lingxin(lxchen@yic.ac.cn) |
| Abstract | Sulfamethoxazole (SMX) has attracted much attention due to its high probability of detection in the environment. Marine bacteria Vibrio diabolicus strain L2-2 has been proven to be able to transform SMX. In this study, the potential resistance and biotransformation mechanism of strain L2-2 to SMX, and key genes responses to SMX at environmental concentrations were researched. KEGG pathways were enriched by down-regulated genes including degradation of L-Leucine, L-Isoleucine, and fatty acid metabolism. Resistance mechanism could be concluded as the enhancement of membrane transport, antioxidation, response regulator, repair proteins, and ribosome protection. Biotransformation genes might involve in arylamine N-acetyltransferases (nat), cytochrome c553 (cyc-553) and acyl-CoA synthetase (acs). At the environmental concentration of SMX (0.1-10 mu g/L), nat was not be activated, which meant the acetylation of SMX might not occur in the environment; however, cyc-553 was up-regulated under SMX stress of 1 mu g/L, which indicated the hydroxylation of SMX could occur in the environment. Besides, the membrane transport and antioxidation of strain L2-2 could be activated under SMX stress of 10 mu g/L. The results provided a better understanding of resistance and biotransformation of bacteria to SMX and would support related researches about the impacts of environmental antibiotics. |
| Funding Organization | National Natural Science Foundation of China ; Taishan Scholar Project Special Funding of China |
| Indexed By | SCI |
| Language | 英语 |
| WOS Research Area | Engineering ; Environmental Sciences & Ecology |
| WOS ID | WOS:000638080300006 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.yic.ac.cn/handle/133337/27227 |
| Collection | 中科院海岸带环境过程与生态修复重点实验室_海岸带环境过程实验室 中科院海岸带环境过程与生态修复重点实验室 |
| Corresponding Author | Chen, Lingxin |
| Affiliation | 1.Chinese Acad Sci, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Shandong Prov Key Lab Coastal Environm Proc, Yantai Inst Coastal Zone Res,Res Ctr Coastal Envi, Yantai 264003, Peoples R China 2.Pilot Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China 4.Binzhou Med Univ, Sch Pharm, Yantai 264003, Peoples R China 5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| Recommended Citation GB/T 7714 | Wang, Qiaoning,Wang, Hongdan,Jiang, Yaru,et al. Biotransformation mechanism of Vibrio diabolicus to sulfamethoxazole at transcriptional level[J]. JOURNAL OF HAZARDOUS MATERIALS,2021,411:10. |
| APA | Wang, Qiaoning,Wang, Hongdan,Jiang, Yaru,Lv, Min,Wang, Xiaoyan,&Chen, Lingxin.(2021).Biotransformation mechanism of Vibrio diabolicus to sulfamethoxazole at transcriptional level.JOURNAL OF HAZARDOUS MATERIALS,411,10. |
| MLA | Wang, Qiaoning,et al."Biotransformation mechanism of Vibrio diabolicus to sulfamethoxazole at transcriptional level".JOURNAL OF HAZARDOUS MATERIALS 411(2021):10. |
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| Biotransformation me（3481KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View Download | |
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