| Molecular characterization of myostatin and its inhibitory function on myogenesis and muscle growth in Chinese Shrimp, Fenneropenaeus chinensis | |
Yunjun Yan; Xia Lu ; Jie Kong; Xianhong Meng; Sheng Luan; Ping Dai; Baolong Chen; Baoxiang Cao; Kun Luo
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| 发表期刊 | Gene
 |
| ISSN | 0378-1119 |
| 2020 | |
| 卷号 | 758页码:144986 |
| 关键词 | Myostatin Myogenesis Muscle growth RNAi Negative regulator Fenneropenaeus chinensis |
| DOI | 10.1016/j.gene.2020.144986 |
| 通讯作者 | Lu, Xia(luxia@ysfri.ac.cn) |
| 英文摘要 | Myostatin (Mstn) inhibits muscle growth in vertebrates with endoskeleton, but it is still inconclusive that Mstn is a positive or negative regulator in crustacean with exoskeleton, and little information was available for its function on myogenesis. In this study, we identified and characterized the Mstn from Fenneropenaeus chinensis (FcMstn), and investigated its function on myogenesis and muscle growth. Two different cDNA sequences (2628 bp and 2604 bp) encoding for slightly different sizes of proteins were obtained for FcMstn, containing 86 bp of 5' untranslated regions (UTR) and 1258 bp of 3' UTR. The open reading frame of the long sequence and the short sequence contain 1284 bp and 1260 bp cDNA, encoding 427 and 419 amino acid sequence, respectively. Sequence analysis revealed that the overall protein sequence and specific functional sites of FcMstn were highly conserved with those in other crustacean species. In the early development stage, the muscle firstly appeared in nauplius stage and developed gradually until post larval, but the expression of FcMstn at mRNA and protein levels decreased from nauplius stage to post larval stage, indicating that Mstn involved in myogenesis as a negative regulator in shrimp. In the adult shrimp, the expression of FcMstn at mRNA and protein levels in muscle were significantly lower in the larger group than in the smaller group, and the diameter and number of muscle fiber of the muscle were significantly different between the two groups. Moreover, the shrimp with reduced level of FcMstn by RNAi displayed a dramatic faster growth rate compared with the control group. The present study demonstrates that FcMstn involved in myogenesis and muscle growth probably also as a negative regulator in shrimp like in vertebrates. |
| 资助机构 | National Key Research and Development Program of China ; National Natural Science Foundation of China ; Central Public interest Scientific Institution Basal Research Fund, Yellow Sea Fisheries Research Institute, CAFS |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | EXPRESSION ANALYSIS ; CDNA CLONING ; MSTN GENE ; TRAITS ; POLYMORPHISMS ; HYPERTROPHY ; ASSOCIATION ; PERFORMANCE ; MUTATIONS ; MASS |
| 研究领域[WOS] | Genetics & Heredity |
| WOS记录号 | WOS:000564694000002 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/26161 |
| 专题 | 海岸带生物学与生物资源利用重点实验室 海岸带生物学与生物资源利用重点实验室_海岸带生物资源高效利用研究与发展中心 |
| 通讯作者 | Yunjun Yan |
| 推荐引用方式 GB/T 7714 | Yunjun Yan,Xia Lu,Jie Kong,et al. Molecular characterization of myostatin and its inhibitory function on myogenesis and muscle growth in Chinese Shrimp, Fenneropenaeus chinensis[J]. Gene,2020,758:144986. |
| APA | Yunjun Yan.,Xia Lu.,Jie Kong.,Xianhong Meng.,Sheng Luan.,...&Kun Luo.(2020).Molecular characterization of myostatin and its inhibitory function on myogenesis and muscle growth in Chinese Shrimp, Fenneropenaeus chinensis.Gene,758,144986. |
| MLA | Yunjun Yan,et al."Molecular characterization of myostatin and its inhibitory function on myogenesis and muscle growth in Chinese Shrimp, Fenneropenaeus chinensis".Gene 758(2020):144986. |
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