LuxR-Type Regulator AclR1 of Azorhizobium caulinodans Regulates Cyclic di-GMP and Numerous Phenotypes in Free-Living and Symbiotic States
Liu, W; Li, Y; Bai, X; Wu, HG; Bian, LX; Hu, XK1,2
Source PublicationMOLECULAR PLANT-MICROBE INTERACTIONS
ISSN0894-0282
2020
Volume33Issue:3Pages:528-538
Keywordprotein system nodulation
MOST Discipline CatalogueBiochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Plant Sciences
DOI10.1094/MPMI-10-19-0306-R
Department海岸带生物学与生物资源保护实验室
Abstract

LuxR-type regulators play important roles in transcriptional regulation in bacteria and control various biological processes. A genome sequence analysis showed the existence of seven LuxR-type regulators in Azorhizobium caulinodans ORS571, an important nitrogen-fixing bacterium in both its free-living state and in symbiosis with its host, Sesbania rostrata. However, the functional mechanisms of these regulators remain unclear. In this study, we identified a LuxR-type regulator that contains a cheY-homologous receiver (REC) domain in its N terminus and designated it AclR1. Interestingly, phylogenetic analysis revealed that AclR1 exhibited relatively close evolutionary relationships with MalT/GerE/FixJ/NarL family proteins. Functional analysis of an aclR1 deletion mutant (Delta aclR1) in the free-living state showed that AclR1 positively regulated cell motility and flocculation but negatively regulated exopoly-saccharide production, biofilm formation, and second messenger cyclic diguanylate (c-di-GMP)-related gene expression. In the symbiotic state, the Delta aclR1 mutant was defective in competitive colonization and nodulation on host plants. These results suggested that AclR1 could provide bacteria with the ability to compete effectively for symbiotic nodulation. Overall, our results show that the REC-LuxR-type regulator AclR1 regulates numerous phenotypes both in the free-living state and during host plant symbiosis.

SubtypeArticle
Indexed BySCI
Language英语
WOS Keywordprotein ; system ; nodulation
WOS Research AreaBiochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Plant Sciences
WOS IDWOS:000551075100015
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Document Type期刊论文
Identifierhttp://ir.yic.ac.cn/handle/133337/25288
Collection海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
Corresponding AuthorHu, XK
Affiliation1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Bioresource Utilizat, Yantai, Peoples R China;
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China;
3.Yantai Univ, Coll Life Sci, Yantai, Peoples R China
Recommended Citation
GB/T 7714
Liu, W,Li, Y,Bai, X,et al. LuxR-Type Regulator AclR1 of Azorhizobium caulinodans Regulates Cyclic di-GMP and Numerous Phenotypes in Free-Living and Symbiotic States[J]. MOLECULAR PLANT-MICROBE INTERACTIONS,2020,33(3):528-538.
APA Liu, W,Li, Y,Bai, X,Wu, HG,Bian, LX,&Hu, XK.(2020).LuxR-Type Regulator AclR1 of Azorhizobium caulinodans Regulates Cyclic di-GMP and Numerous Phenotypes in Free-Living and Symbiotic States.MOLECULAR PLANT-MICROBE INTERACTIONS,33(3),528-538.
MLA Liu, W,et al."LuxR-Type Regulator AclR1 of Azorhizobium caulinodans Regulates Cyclic di-GMP and Numerous Phenotypes in Free-Living and Symbiotic States".MOLECULAR PLANT-MICROBE INTERACTIONS 33.3(2020):528-538.
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