Enhanced biodegradation of crude oil by constructed bacterial consortium comprising salt-tolerant petroleum degraders and biosurfactant producers
Chen, WW; Kong, YC; Li, JD; Sun, YY; Min, J; Hu, XK1,3
Source PublicationINTERNATIONAL BIODETERIORATION & BIODEGRADATION
ISSN0964-8305
2020-08-08
Volume154Pages:105047
Keywordcontaminated soil gene-expression degradation bioremediation bioaugmentation hydrocarbons strains efficiency coastal alkanes
MOST Discipline CatalogueBiotechnology & Applied Microbiology ; Environmental Sciences
DOI10.1016/j.ibiod.2020.105047
Department海岸带生物学与生物资源保护实验室
Abstract

Bioremediation is an attractive strategy of utilizing bacteria to remove crude oil contaminants. In this study, two salt-tolerant crude oil-degrading and biosurfactant-producing bacteria, Dietzia sp. CN-3 and Acinetobacter sp. HC8-3S, were functionally combined to construct a bacterial consortium. The consortium achieved 95.8% degradation efficiency of crude oil in 10 days and various n-alkanes, cycloalkanes, branched alkanes and aromatic hydrocarbons were all depleted more effectively than single strains. Functional optimization of the consortium degraded crude oil efficiently in a wide range of pH (4-10) and salinity (0-120 g L-1). Furthermore, two alkane hydroxylase genes, alkB in CN-3 and alkM in HC8-3S, were cloned and their expression were examined by real-time quantitative polymerase chain reaction, indicating that alkB was more prominent in long-chain alkanes (C-20, C-24 and C-26) utilization and alkM played crucial roles in medium- and long-chain alkanes (C-14, C-16, C-20, C-24 and C-26) degradation. In soil microcosms artificially contaminated with crude oil and bioaugmented with the consortium, 58.3% of total petroleum hydrocarbons were depleted after 60 days and the degradation rate (485.8 mg kg(-1) d(-1)) was higher than those reported in previous studies. Consequently, the consortium is a promising candidate in crude oil bioremediation.

SubtypeArticle
Indexed BySCI
Language英语
WOS Keywordcontaminated soil ; gene-expression ; degradation ; bioremediation ; bioaugmentation ; hydrocarbons ; strains ; efficiency ; coastal ; alkanes
WOS Research AreaBiotechnology & Applied Microbiology ; Environmental Sciences
WOS IDWOS:000576666100002
Citation statistics
Document Type期刊论文
Identifierhttp://ir.yic.ac.cn/handle/133337/25281
Collection海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
Corresponding AuthorHu, XK
Affiliation1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Bioresource Utilizat, Chunhui Rd, Yantai 264003, Peoples R China;
2.Univ Chinese Acad Sci, Beijing, Peoples R China;
3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China;
4.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao, Peoples R China
Recommended Citation
GB/T 7714
Chen, WW,Kong, YC,Li, JD,et al. Enhanced biodegradation of crude oil by constructed bacterial consortium comprising salt-tolerant petroleum degraders and biosurfactant producers[J]. INTERNATIONAL BIODETERIORATION & BIODEGRADATION,2020,154:105047.
APA Chen, WW,Kong, YC,Li, JD,Sun, YY,Min, J,&Hu, XK.(2020).Enhanced biodegradation of crude oil by constructed bacterial consortium comprising salt-tolerant petroleum degraders and biosurfactant producers.INTERNATIONAL BIODETERIORATION & BIODEGRADATION,154,105047.
MLA Chen, WW,et al."Enhanced biodegradation of crude oil by constructed bacterial consortium comprising salt-tolerant petroleum degraders and biosurfactant producers".INTERNATIONAL BIODETERIORATION & BIODEGRADATION 154(2020):105047.
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