Comparative Transcriptomics Reveals Distinct Gene Expressions of a Model Ciliated Protozoan Feeding on Bacteria-Free Medium, Digestible, and Digestion-Resistant Bacteria
Zou, Songbao; Zhang, Qianqian; Gong, Jun
Source PublicationMICROORGANISMS
2020-04
Volume8Issue:4Pages:559
KeywordGLYOXYLATE CYCLE BACILLUS-SUBTILIS ISOCITRATE LYASE MARINE PROTISTS WATER-COLUMN FOOD QUALITY GLUTATHIONE SEQUENCE SURVIVAL DIFFERENTIATION
MOST Discipline CatalogueMicrobiology
DOI10.3390/microorganisms8040559
Contribution Rank[Zou, Songbao; Zhang, Qianqian] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China; [Zou, Songbao; Zhang, Qianqian] Univ Chinese Acad Sci, 19 Yuquan Rd, Beijing 100049, Peoples R China; [Zou, Songbao; Zhang, Qianqian] Chinese Acad Sci, Ctr Ocean Megasci, 7 Nanhai Rd, Qingda 266071, Peoples R China; [Gong, Jun] Sun Yat Sen Univ, Sch Marine Sci, Zhuhai Campus, Tangjiawan 519000, Zhuhai, Peoples R China; [Gong, Jun] Southern Marine Sci & Engn Guangdong Lab Zhuhai, Zhuhai 519000, Peoples R China
Department海岸带生物资源高效利用研究与发展中心
AbstractBacterivory is an important ecological function of protists in natural ecosystems. However, there are diverse bacterial species resistant to protistan digestion, which reduces the carbon flow to higher trophic levels. So far, a molecular biological view of metabolic processes in heterotrophic protists during predation of bacterial preys of different digestibility is still lacking. In this study, we investigated the growth performance a ciliated protozoan Tetrahymena thermophila cultivated in a bacteria-free Super Proteose Peptone (SPP) medium (control), and in the media mixed with either a digestion-resistant bacterial species (DRB) or a digestible strain of E. coli (ECO). We found the protist population grew fastest in the SPP and slowest in the DRB treatment. Fluorescence in situ hybridization confirmed that there were indeed non-digested, viable bacteria in the ciliate cells fed with DRB, but none in other treatments. Comparative analysis of RNA-seq data showed that, relative to the control, 637 and 511 genes in T. thermophila were significantly and differentially expressed in the DRB and ECO treatments, respectively. The protistan expression of lysosomal proteases (especially papain-like cysteine proteinases), GH18 chitinases, and an isocitrate lyase were upregulated in both bacterial treatments. The genes encoding protease, glycosidase and involving glycolysis, TCA and glyoxylate cycles of carbon metabolic processes were higher expressed in the DRB treatment when compared with the ECO. Nevertheless, the genes for glutathione metabolism were more upregulated in the control than those in both bacterial treatments, regardless of the digestibility of the bacteria. The results of this study indicate that not only bacterial food but also digestibility of bacterial taxa modulate multiple metabolic processes in heterotrophic protists, which contribute to a better understanding of protistan bacterivory and bacteria-protists interactions on a molecular basis.
SubtypeArticle
Indexed BySCI
Language英语
WOS KeywordGLYOXYLATE CYCLE ; BACILLUS-SUBTILIS ; ISOCITRATE LYASE ; MARINE PROTISTS ; WATER-COLUMN ; FOOD QUALITY ; GLUTATHIONE ; SEQUENCE ; SURVIVAL ; DIFFERENTIATION
WOS IDWOS:000533510400042
Funding OrganizationMarine S&T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology (Qingdao) [2018SDKJ0406-4] ; Key Research Project of Frontier Science, CAS [QYZDB-SSW-DQC013-1] ; Youth Innovation Promotion Association, CAS [2019216] ; Natural Science Foundation of ChinaNational Natural Science Foundation of China [31672251, 41976128]
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.yic.ac.cn/handle/133337/25056
Collection海岸带生物学与生物资源利用重点实验室_海岸带生物资源高效利用研究与发展中心
Affiliation1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China;
2.Univ Chinese Acad Sci, 19 Yuquan Rd, Beijing 100049, Peoples R China;
3.Chinese Acad Sci, Ctr Ocean Megasci, 7 Nanhai Rd, Qingda 266071, Peoples R China;
4.Sun Yat Sen Univ, Sch Marine Sci, Zhuhai Campus, Tangjiawan 519000, Zhuhai, Peoples R China;
5.Southern Marine Sci & Engn Guangdong Lab Zhuhai, Zhuhai 519000, Peoples R China
Recommended Citation
GB/T 7714
Zou, Songbao,Zhang, Qianqian,Gong, Jun. Comparative Transcriptomics Reveals Distinct Gene Expressions of a Model Ciliated Protozoan Feeding on Bacteria-Free Medium, Digestible, and Digestion-Resistant Bacteria[J]. MICROORGANISMS,2020,8(4):559.
APA Zou, Songbao,Zhang, Qianqian,&Gong, Jun.(2020).Comparative Transcriptomics Reveals Distinct Gene Expressions of a Model Ciliated Protozoan Feeding on Bacteria-Free Medium, Digestible, and Digestion-Resistant Bacteria.MICROORGANISMS,8(4),559.
MLA Zou, Songbao,et al."Comparative Transcriptomics Reveals Distinct Gene Expressions of a Model Ciliated Protozoan Feeding on Bacteria-Free Medium, Digestible, and Digestion-Resistant Bacteria".MICROORGANISMS 8.4(2020):559.
Files in This Item:
File Name/Size DocType Version Access License
Comparative Transcri(5545KB)期刊论文出版稿开放获取CC BY-NC-SAView Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Zou, Songbao]'s Articles
[Zhang, Qianqian]'s Articles
[Gong, Jun]'s Articles
Baidu academic
Similar articles in Baidu academic
[Zou, Songbao]'s Articles
[Zhang, Qianqian]'s Articles
[Gong, Jun]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Zou, Songbao]'s Articles
[Zhang, Qianqian]'s Articles
[Gong, Jun]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Comparative Transcriptomics Reveals Distinct Gene Expressions of a Model Ciliated Protozoan Feeding on Bacteria-Free Medium, Digestible, and Digestion-Resistant Bacteria.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.