Cloning, expression, and characterization of a novel plant type cryptochrome gene from the green alga Haematococcus pluvialis
Hang, Wei; Gujar, Asadullah; Zhang, Hongjiang; Xu, Wenxin; Zhao, Chunchao; Zhu, Xiaoli; Xue, Jinai; Zhang, Chunhui; Ji, Chunli; Qin, Song; Li, Runzhi; Cui, Hongli
Source PublicationPROTEIN EXPRESSION AND PURIFICATION
2020-08
Volume172Pages:105633
KeywordBLUE-LIGHT PHOTORECEPTORS PHOTOLYASE/CRYPTOCHROME FAMILY ASTAXANTHIN ACCUMULATION DNA-REPAIR ARABIDOPSIS PHOTOACTIVATION PHOTOLYASE EVOLUTION MEMBERS LIFE
MOST Discipline CatalogueBiochemical Research Methods ; Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
DOI10.1016/j.pep.2020.105633
Contribution Rank[Hang, Wei; Gujar, Asadullah; Zhang, Hongjiang; Xu, Wenxin; Zhao, Chunchao; Zhu, Xiaoli; Xue, Jinai; Zhang, Chunhui; Ji, Chunli; Li, Runzhi; Cui, Hongli] Shanxi Agr Univ, Inst Mol Agr & Bioenergy, Taigu 030801, Peoples R China; [Qin, Song] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Biol Resources Utilizat, Yantai 264003, Peoples R China
Department海岸带生物学与生物资源保护实验室
AbstractA full-length cDNA sequence of plant type CRY (designated Hae-P-CRY) was cloned from the green alga Haematococcus pluvialis. The cDNA sequence was 3608 base pairs (bp) in length, which contained a 2988-bp open reading frame encoding 995 amino acids with molecular mass of 107.7 kDa and isoelectric point of 6.19. Multiple alignment analysis revealed that the deduced amino acid sequence of Hae-P-CRY shared high identity of 47-66% with corresponding plant type CRYs from other eukaryotes. The catalytic motifs of plant type CRYs were detected in the amino acid sequence of Hae-P-CRY including the typical PHR and CTE domains. Phylogenetic analysis showed that the Hae-P-CRY was grouped together with other plant type CRYs from green algae and higher plants, which distinguished from other distinct groups. The transcriptional level of Hae-P-CRY was strongly decreased after 0-4 h under HL stress. In addition, the Hae-P-CRY gene was heterologously expressed in Escherichia coli BL21 (DE3) and successfully purified. The typical spectroscopic characteristics of plant type CRYs were present in Hae-P-CRY indicated that it may be an active enzyme, which provided valuable clue for further functional investigation in the green alga H. pluvialis. These results lay the foundation for further function and interaction protein identification involved in CRYs mediated signal pathway under HL stress in H. pluvialis.
SubtypeArticle
Indexed BySCI
Language英语
WOS KeywordBLUE-LIGHT PHOTORECEPTORS ; PHOTOLYASE/CRYPTOCHROME FAMILY ; ASTAXANTHIN ACCUMULATION ; DNA-REPAIR ; ARABIDOPSIS ; PHOTOACTIVATION ; PHOTOLYASE ; EVOLUTION ; MEMBERS ; LIFE
WOS IDWOS:000531018500005
Citation statistics
Document Type期刊论文
Identifierhttp://ir.yic.ac.cn/handle/133337/25011
Collection海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
Affiliation1.Shanxi Agr Univ, Inst Mol Agr & Bioenergy, Taigu 030801, Peoples R China;
2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Biol Resources Utilizat, Yantai 264003, Peoples R China
Recommended Citation
GB/T 7714
Hang, Wei,Gujar, Asadullah,Zhang, Hongjiang,et al. Cloning, expression, and characterization of a novel plant type cryptochrome gene from the green alga Haematococcus pluvialis[J]. PROTEIN EXPRESSION AND PURIFICATION,2020,172:105633.
APA Hang, Wei.,Gujar, Asadullah.,Zhang, Hongjiang.,Xu, Wenxin.,Zhao, Chunchao.,...&Cui, Hongli.(2020).Cloning, expression, and characterization of a novel plant type cryptochrome gene from the green alga Haematococcus pluvialis.PROTEIN EXPRESSION AND PURIFICATION,172,105633.
MLA Hang, Wei,et al."Cloning, expression, and characterization of a novel plant type cryptochrome gene from the green alga Haematococcus pluvialis".PROTEIN EXPRESSION AND PURIFICATION 172(2020):105633.
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