| Cloning, expression, and characterization of a novel plant type cryptochrome gene from the green alga Haematococcus pluvialis | |
Hang, Wei; Gujar, Asadullah; Zhang, Hongjiang; Xu, Wenxin; Zhao, Chunchao; Zhu, Xiaoli; Xue, Jinai; Zhang, Chunhui; Ji, Chunli; Qin, Song ; Li, Runzhi; Cui, Hongli
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| Source Publication | PROTEIN EXPRESSION AND PURIFICATION
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| 2020-08 | |
| Volume | 172Pages:105633 |
| Keyword | BLUE-LIGHT PHOTORECEPTORS PHOTOLYASE/CRYPTOCHROME FAMILY ASTAXANTHIN ACCUMULATION DNA-REPAIR ARABIDOPSIS PHOTOACTIVATION PHOTOLYASE EVOLUTION MEMBERS LIFE |
| MOST Discipline Catalogue | Biochemical Research Methods ; Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
| DOI | 10.1016/j.pep.2020.105633 |
| Contribution Rank | [Hang, Wei; Gujar, Asadullah; Zhang, Hongjiang; Xu, Wenxin; Zhao, Chunchao; Zhu, Xiaoli; Xue, Jinai; Zhang, Chunhui; Ji, Chunli; Li, Runzhi; Cui, Hongli] Shanxi Agr Univ, Inst Mol Agr & Bioenergy, Taigu 030801, Peoples R China; [Qin, Song] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Biol Resources Utilizat, Yantai 264003, Peoples R China |
| Department | 海岸带生物学与生物资源保护实验室 |
| Abstract | A full-length cDNA sequence of plant type CRY (designated Hae-P-CRY) was cloned from the green alga Haematococcus pluvialis. The cDNA sequence was 3608 base pairs (bp) in length, which contained a 2988-bp open reading frame encoding 995 amino acids with molecular mass of 107.7 kDa and isoelectric point of 6.19. Multiple alignment analysis revealed that the deduced amino acid sequence of Hae-P-CRY shared high identity of 47-66% with corresponding plant type CRYs from other eukaryotes. The catalytic motifs of plant type CRYs were detected in the amino acid sequence of Hae-P-CRY including the typical PHR and CTE domains. Phylogenetic analysis showed that the Hae-P-CRY was grouped together with other plant type CRYs from green algae and higher plants, which distinguished from other distinct groups. The transcriptional level of Hae-P-CRY was strongly decreased after 0-4 h under HL stress. In addition, the Hae-P-CRY gene was heterologously expressed in Escherichia coli BL21 (DE3) and successfully purified. The typical spectroscopic characteristics of plant type CRYs were present in Hae-P-CRY indicated that it may be an active enzyme, which provided valuable clue for further functional investigation in the green alga H. pluvialis. These results lay the foundation for further function and interaction protein identification involved in CRYs mediated signal pathway under HL stress in H. pluvialis. |
| Subtype | Article |
| Indexed By | SCI |
| Language | 英语 |
| WOS Keyword | BLUE-LIGHT PHOTORECEPTORS ; PHOTOLYASE/CRYPTOCHROME FAMILY ; ASTAXANTHIN ACCUMULATION ; DNA-REPAIR ; ARABIDOPSIS ; PHOTOACTIVATION ; PHOTOLYASE ; EVOLUTION ; MEMBERS ; LIFE |
| WOS ID | WOS:000531018500005 |
| Funding Organization | National Natural Science Foundation of ChinaNational Natural Science Foundation of China [31902394] ; Applying Basic Research Planning Project of Shanxi Province [201801D221250] ; Key Research and Development Planning Project of Shanxi Province [201803D31063] ; Science and Technology Innovation Planning Project of Shanxi Agricultural University [2018YJ16] ; Key Research and Planning Project of Jinzhong City [Y192012] ; Graduate Education Innovation Project of Shanxi province [2019SY226] ; Shanxi Scholarship Council of China [2015-064] ; Key Project of the Key Research and Development Program of Shanxi Province, China [201603D312005] |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.yic.ac.cn/handle/133337/25011 |
| Collection | 海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室 |
| Affiliation | 1.Shanxi Agr Univ, Inst Mol Agr & Bioenergy, Taigu 030801, Peoples R China; 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Biol Resources Utilizat, Yantai 264003, Peoples R China |
| Recommended Citation GB/T 7714 | Hang, Wei,Gujar, Asadullah,Zhang, Hongjiang,et al. Cloning, expression, and characterization of a novel plant type cryptochrome gene from the green alga Haematococcus pluvialis[J]. PROTEIN EXPRESSION AND PURIFICATION,2020,172:105633. |
| APA | Hang, Wei.,Gujar, Asadullah.,Zhang, Hongjiang.,Xu, Wenxin.,Zhao, Chunchao.,...&Cui, Hongli.(2020).Cloning, expression, and characterization of a novel plant type cryptochrome gene from the green alga Haematococcus pluvialis.PROTEIN EXPRESSION AND PURIFICATION,172,105633. |
| MLA | Hang, Wei,et al."Cloning, expression, and characterization of a novel plant type cryptochrome gene from the green alga Haematococcus pluvialis".PROTEIN EXPRESSION AND PURIFICATION 172(2020):105633. |
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| Cloning, expression,（13070KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View Download | |
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