SERS-based droplet microfluidics for high-throughput gradient analysis | |
Jeon, J; Choi, N; Chen, H; Moon, JI; Chen, LX; Choo, J | |
发表期刊 | LAB ON A CHIP |
ISSN | 1473-0197 |
2019-02-21 | |
卷号 | 19期号:4页码:674-681 |
关键词 | ENHANCED RAMAN-SCATTERING GOLD NANOFLOWERS IMMUNOASSAY PLATFORMS |
研究领域 | Biochemical Research Methods ; Chemistry, Multidisciplinary ; Chemistry, Analytical ; Nanoscience & Nanotechnology ; Instruments & Instrumentation |
DOI | 10.1039/c8lc01180j |
产权排序 | [Jeon, Jinhyeok; Choi, Namhyun; Chen, Hao; Moon, Joung-Il] Hanyang Univ, Dept Bionano Technol, Ansan 15588, South Korea; [Chen, Hao; Moon, Joung-Il; Choo, Jaebum] Chung Ang Univ, Dept Chem, Seoul 06974, South Korea; [Chen, Lingxin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China |
作者部门 | 海岸带环境工程技术研究与发展中心 |
英文摘要 | In the last two decades, microfluidic technology has emerged as a highly efficient tool for the study of various chemical and biological reactions. Recently, we reported that high-throughput detection of various concentrations of a reagent is possible using a continuous gradient microfluidic channel combined with a surface-enhanced Raman scattering (SERS) detection platform. In this continuous flow regime, however, the deposition of nanoparticle aggregates on channel surfaces induces the memory effect, affecting both sensitivity and reproducibility. To resolve this problem, a SERS-based gradient droplet system was developed. Herein, the serial dilution of a reagent was achieved in a stepwise manner using microfluidic concentration gradient generators. Then various concentrations of a reagent generated in different channels were simultaneously trapped into the tiny volume of droplets by injecting an oil mixture into the channel. Compared to the single-phase regime, this two-phase liquid/liquid segmented flow regime allows minimization of resident time distributions of reagents through localization of reagents in encapsulated droplets. Consequently, the sample stacking problem could be solved using this system because it greatly reduces the memory effect. We believe that this SERS-based gradient droplet system will be of significant utility in simultaneously monitoring chemical and biological reactions for various concentrations of a reagent. |
文章类型 | Article |
资助机构 | National Research Foundation of KoreaNational Research Foundation of Korea [2017M3D1A1039287, 2018M3A7B4071203] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China [41776110, 21575159] |
收录类别 | SCI |
语种 | 英语 |
关键词[WOS] | ENHANCED RAMAN-SCATTERING ; GOLD NANOFLOWERS ; IMMUNOASSAY ; PLATFORMS |
研究领域[WOS] | Biochemical Research Methods ; Chemistry, Multidisciplinary ; Chemistry, Analytical ; Nanoscience & Nanotechnology ; Instruments & Instrumentation |
WOS记录号 | WOS:000459726400011 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.yic.ac.cn/handle/133337/24994 |
专题 | 中国科学院海岸带环境过程与生态修复重点实验室_海岸带环境工程技术研究与发展中心 中国科学院海岸带环境过程与生态修复重点实验室 |
作者单位 | 1.Hanyang Univ, Dept Bionano Technol, Ansan 15588, South Korea; 2.Chung Ang Univ, Dept Chem, Seoul 06974, South Korea; 3.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China |
推荐引用方式 GB/T 7714 | Jeon, J,Choi, N,Chen, H,et al. SERS-based droplet microfluidics for high-throughput gradient analysis[J]. LAB ON A CHIP,2019,19(4):674-681. |
APA | Jeon, J,Choi, N,Chen, H,Moon, JI,Chen, LX,&Choo, J.(2019).SERS-based droplet microfluidics for high-throughput gradient analysis.LAB ON A CHIP,19(4),674-681. |
MLA | Jeon, J,et al."SERS-based droplet microfluidics for high-throughput gradient analysis".LAB ON A CHIP 19.4(2019):674-681. |
条目包含的文件 | 下载所有文件 | |||||
文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
SERS-based droplet m(2170KB) | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | 浏览 下载 |
个性服务 |
推荐该条目 |
保存到收藏夹 |
查看访问统计 |
导出为Endnote文件 |
谷歌学术 |
谷歌学术中相似的文章 |
[Jeon, J]的文章 |
[Choi, N]的文章 |
[Chen, H]的文章 |
百度学术 |
百度学术中相似的文章 |
[Jeon, J]的文章 |
[Choi, N]的文章 |
[Chen, H]的文章 |
必应学术 |
必应学术中相似的文章 |
[Jeon, J]的文章 |
[Choi, N]的文章 |
[Chen, H]的文章 |
相关权益政策 |
暂无数据 |
收藏/分享 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论