A graphene oxide/gold nanoparticle-based amplification method for SERS immunoassay of cardiac troponin I
Fu, XL; Wang, YQ; Liu, YM; Liu, HT; Fu, LW; Wen, JH; Li, JW; Wei, PH; Chen, LX
Source PublicationANALYST
ISSN0003-2654
2019-03-07
Volume144Issue:5Pages:1582-1589
KeywordACUTE MYOCARDIAL-INFARCTION DISPERSED CARBON NANOTUBES PEROXIDASE-LIKE CATALYSIS SURFACE-ENHANCED RAMAN ULTRASENSITIVE DETECTION COLORIMETRIC SENSOR OPTICAL NANOPROBES SILVER DEPOSITION ASSAY BIOSENSORS
MOST Discipline CatalogueChemistry, Analytical
DOI10.1039/c8an02022a
Contribution Rank[Fu, Xiuli; Liu, Yongming; Liu, Huitao; Wen, Jiahui; Li, Jingwen; Chen, Lingxin] Yantai Univ, Sch Chem & Chem Engn, Yantai 264005, Peoples R China; [Wang, Yunqing; Fu, Longwen; Chen, Lingxin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China; [Wei, Peihai] Qilu Normal Univ, Sch Chem & Chem Engn, 36 Lishan Rd, Jinan 250013, Shandong, Peoples R China
Department海岸带环境工程技术研究与发展中心
AbstractCardiac troponin I (cTnI) was considered as the gold standard for acute myocardial infarction (AMI) diagnosis owing to its superior cardiac specificity for cardiac damage and showing little or no changes in patients with a skeletal muscle disease or trauma. Herein, a new signal amplification surface-enhanced Raman scattering (SERS) platform was developed for recognition and detection of cTnI by using gold nanoparticles (AuNPs), graphene oxide (GO) and magnetic beads (MB). Here, antibody/Raman reporter labeled AuNP-functionalized GO were employed as both SERS nanotags and signal amplification carriers. Monoclonal antibody modified MB were applied as the capture probe and separation agents. In the presence of cTnI, sandwich type immunocomplexes, capture probe/target/SERS nanotags, were formed through antibody-antigen-antibody interactions. Due to the strong SERS enhancement ability of the designed GO/AuNP complexes and a high binding chance between cTnI and the GO/AuNP complexes, the proposed SERS-based immunoassay could selectively detect cTnI with a high sensitivity (detection limit of 5 pg mL(-1)) and a good linearity was obtained in a range of 0.01-1000 ng mL(-1). In addition, this method was also successfully applied for detecting cTnI in serum substitute media with a similar linear range. Furthermore, this strategy can be constructed with different kinds of antibodies and Raman reporters, and thus can be easily used for simultaneous detection of multiple biomarkers. Therefore, this proposed SERS-based signal amplification technique shows strong potential for the clinical diagnosis of AMI disease.
SubtypeArticle
Indexed BySCI
Language英语
WOS KeywordACUTE MYOCARDIAL-INFARCTION ; DISPERSED CARBON NANOTUBES ; PEROXIDASE-LIKE CATALYSIS ; SURFACE-ENHANCED RAMAN ; ULTRASENSITIVE DETECTION ; COLORIMETRIC SENSOR ; OPTICAL NANOPROBES ; SILVER DEPOSITION ; ASSAY ; BIOSENSORS
WOS Research AreaChemistry, Analytical
WOS IDWOS:000461614000008
Funding OrganizationNational Natural Science Foundation of Shandong Province in China [ZR2017BB026] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China [21705139, 21275158, 21575159] ; Shandong Provincial Natural Science FoundationNatural Science Foundation of Shandong Province [ZR2014BL031]
Citation statistics
Cited Times:45[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.yic.ac.cn/handle/133337/24976
Collection中科院海岸带环境过程与生态修复重点实验室_海岸带环境工程技术研究与发展中心
Affiliation1.Yantai Univ, Sch Chem & Chem Engn, Yantai 264005, Peoples R China;
2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China;
3.Qilu Normal Univ, Sch Chem & Chem Engn, 36 Lishan Rd, Jinan 250013, Shandong, Peoples R China
Recommended Citation
GB/T 7714
Fu, XL,Wang, YQ,Liu, YM,et al. A graphene oxide/gold nanoparticle-based amplification method for SERS immunoassay of cardiac troponin I[J]. ANALYST,2019,144(5):1582-1589.
APA Fu, XL.,Wang, YQ.,Liu, YM.,Liu, HT.,Fu, LW.,...&Chen, LX.(2019).A graphene oxide/gold nanoparticle-based amplification method for SERS immunoassay of cardiac troponin I.ANALYST,144(5),1582-1589.
MLA Fu, XL,et al."A graphene oxide/gold nanoparticle-based amplification method for SERS immunoassay of cardiac troponin I".ANALYST 144.5(2019):1582-1589.
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