A near-infrared fluorescent probe for evaluating endogenous hydrogen peroxide during ischemia/reperfusion injury

doi:10.1039/c9an00243j

YIC-IR > 中国科学院海岸带环境过程与生态修复重点实验室 > 海岸带环境工程技术研究与发展中心

	A near-infrared fluorescent probe for evaluating endogenous hydrogen peroxide during ischemia/reperfusion injury
	Xu, RF ; Wang, Y; You, HY ; Zhang, LW; Wang, YQ; Chen, LX
发表期刊	ANALYST
ISSN	0003-2654
	2019-04-21
卷号	144 期号:8 页码:2556-2564
关键词	IMAGING IN-VITRO SUPEROXIDE ANION XANTHINE-OXIDASE RATIONAL DESIGN LIVER ISCHEMIA FREE-RADICALS MITOCHONDRIA OXYGEN ELECTROCATALYSIS PEROXYNITRITE
研究领域	Chemistry, Analytical
DOI	10.1039/c9an00243j
产权排序	[Xu, Runfeng; You, Huiyan] Dalian Univ, Sch Environm & Chem Engn, Dalian 116622, Peoples R China; [Xu, Runfeng; Wang, Yue; Zhang, Liangwei; Wang, Yunqing; Chen, Lingxin] Chinese Acad Sci, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China; [Wang, Yue] Univ Chinese Acad Sci, Beijing 10004, Peoples R China; [Chen, Lingxin] Qufu Normal Univ, Coll Chem & Chem Engn, Qufu 273165, Peoples R China
通讯作者	You, Huiyan(dlyhy@dicp.ac.cn) ; Zhang, Liangwei(liangweizhang@yic.ac.cn) ; Chen, Lingxin(lxchen@yic.ac.cn)
作者部门	海岸带环境工程技术研究与发展中心
英文摘要	Hydrogen peroxide (H2O2), as a major component of reactive oxygen species (ROS), plays an important role in normal physiological processes. A H2O2 burst also occurs in the ischemia/reperfusion (I/R) process and causes a series of physiological and pathological injuries. Therefore, it is important to determine concentration fluctuations of H2O2. Here we develop a ratiometric fluorescent probe, Cy-ArB, which shows high selectivity and sensitivity toward H2O2. The fluorescence response of the probe is triggered by the reaction of borate esters with H2O2, and this process releases a near-infrared heptamethine cyanine fluorophore which has the ability of mitochondrial tracing. Hence, the probe can be used for real-time monitoring of H2O2 fluctuations in the mitochondrial respiration chain. Finally, we explore the fluctuations of H2O2 in cells and in vivo during the I/R process using the probe Cy-ArB. The results of our experiments prove that our probe is a potential candidate for clinical surgery pre-evaluation.
文章类型	Article
资助机构	National Nature Science Foundation of ChinaNational Natural Science Foundation of China [21575159, 21778026, 81573393] ; Program of the Youth Innovation Promotion Association, CAS [2017256, 2019217] ; National Key Scientific Instrument and Equipment Development Projects [2013YQ17052506] ; Instrument Developing Project of the Chinese Academy of Sciences [YZ201662]
收录类别	SCI
语种	英语
关键词[WOS]	IMAGING IN-VITRO ; SUPEROXIDE ANION ; XANTHINE-OXIDASE ; RATIONAL DESIGN ; LIVER ISCHEMIA ; FREE-RADICALS ; MITOCHONDRIA ; OXYGEN ; ELECTROCATALYSIS ; PEROXYNITRITE
研究领域[WOS]	Chemistry, Analytical
WOS记录号	WOS:000467383200008
引用统计	被引频次：23[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/24954
专题	中国科学院海岸带环境过程与生态修复重点实验室_海岸带环境工程技术研究与发展中心海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室中国科学院海岸带环境过程与生态修复重点实验室
作者单位	1.Dalian Univ, Sch Environm & Chem Engn, Dalian 116622, Peoples R China; 2.Chinese Acad Sci, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China; 3.Univ Chinese Acad Sci, Beijing 10004, Peoples R China; 4.Qufu Normal Univ, Coll Chem & Chem Engn, Qufu 273165, Peoples R China
推荐引用方式 GB/T 7714	Xu, RF,Wang, Y,You, HY,et al. A near-infrared fluorescent probe for evaluating endogenous hydrogen peroxide during ischemia/reperfusion injury[J]. ANALYST,2019,144(8):2556-2564.
APA	Xu, RF,Wang, Y,You, HY,Zhang, LW,Wang, YQ,&Chen, LX.(2019).A near-infrared fluorescent probe for evaluating endogenous hydrogen peroxide during ischemia/reperfusion injury.ANALYST,144(8),2556-2564.
MLA	Xu, RF,et al."A near-infrared fluorescent probe for evaluating endogenous hydrogen peroxide during ischemia/reperfusion injury".ANALYST 144.8(2019):2556-2564.

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