A peptidoglycan recognition protein involved in immune recognition and immune defenses in Ruditapes philippinarum
Yang, DL; Han, YJ; Liu, YL; Cao, RW; Wang, Q; Dong, ZJ; Liu, H; Zhang, XL; Zhang, QQ; Zhao, JM
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
2019-05
Volume88Pages:441-448
KeywordRuditapes philippinarum Peptidoglycan recognition protein Immune recognition Immune response
MOST Discipline CatalogueFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
DOI10.1016/j.fsi.2019.03.017
Contribution Rank[Yang, Dinglong; Han, Yijing; Liu, Yongliang; Cao, Ruiwen; Wang, Qing; Dong, Zhijun; Liu, Hui; Zhang, Xiaoli; Zhang, Qianqian; Zhao, Jianmin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Muping Coastal Environm Res Stn, Yantai 264003, Peoples R China; [Yang, Dinglong; Han, Yijing; Wang, Qing; Dong, Zhijun; Liu, Hui; Zhang, Xiaoli; Zhang, Qianqian; Zhao, Jianmin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res & Dev Ctr Efficient Utilizat Coastal Bioresou, Yantai 264003, Peoples R China; [Zhao, Jianmin] Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Shandong, Peoples R China; [Han, Yijing; Cao, Ruiwen] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Department海岸带生物资源高效利用研究与发展中心
AbstractPeptidoglycan recognition proteins (PGRPs) are important pattern recognition receptors in the innate immune system of invertebrates. In the study, a short PGRP (designed as RpPGRP) was identified and characterized from the manila clam Ruditapes philippinamm. The open reading frame of RpPGRP encoded a polypeptide of 249 amino acids with a calculated molecular mass of 27.2 kDa and an isoelectric point of 6.62. Multiple alignments and phylogenetic analysis strongly suggested that RpPGRP was a new member of the PGRP superfamily. In non stimulated clams, RpPGRP exhibited different tissue expression pattern, and highly expressed in hepatopancreas and hemocytes. Expression of RpPGRP transcripts was significantly up -regulated in hemocytes of clams post Vibrio crnguillartzn or Micrococcus luteus challenge. The recombinant RpPGRP (rRpPGRP) exhibited high affinity to PGN, LPS and zymosan in a concentration-dependent manner. With a broad spectrum of bacterial binding activities, rRpPGRP exhibited strong agglutination activity to Escherichia colt, Vibrio splendidus, V. anguiliarum and M. luteus. Furthermore, rRpPGRP exhibited Zn2+ -dependent amidase activity and catalyzed the degradation of insoluble PGN. Especially, rRpPGRP exhibited significant antibacterial activity against E. coil and M. luteus. Moreover, the biofilm formation of E. coil could be inhibited after rRpPGRP incubation in the presence of Zn2+. This inhibitory effect of rRpPGRP might attribute to its amide bactericidal activity. Taken together, rRpPGRP played important roles in PGRP-mediated immune defense mechanisms, especially by recognizing antigens and eliminating bacteria.
Funding OrganizationStrategic Priority Research Program of the Chinese Academy of SciencesChinese Academy of Sciences [XDA23050303] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China [41476126, 41806196] ; Key Research Program of the Chinese Academy of SciencesChinese Academy of Sciences [KZZD-EW-14, KFJ-STS-ZDTP-023] ; Two-Hundred Talents Plan of Yantai [Y839081021] ; Youth Innovation Promotion Association CAS [2016196]
SubtypeArticle
Indexed BySCI
Language英语
WOS KeywordGRAM-NEGATIVE BACTERIA ; L-ALANINE AMIDASE ; MOLECULAR-CLONING ; BINDING PROTEIN ; RECEPTOR ; GENES ; LIPOPOLYSACCHARIDE ; IDENTIFICATION ; PURIFICATION ; EXPRESSION
WOS Research AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS IDWOS:000465060500052
Citation statistics
Document Type期刊论文
Identifierhttp://ir.yic.ac.cn/handle/133337/24948
Collection海岸带生物学与生物资源利用重点实验室_海岸带生物资源高效利用研究与发展中心
支撑部门_中国科学院牟平海岸带环境综合试验站
Affiliation1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Muping Coastal Environm Res Stn, Yantai 264003, Peoples R China;
2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res & Dev Ctr Efficient Utilizat Coastal Bioresou, Yantai 264003, Peoples R China;
3.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Shandong, Peoples R China;
4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Yang, DL,Han, YJ,Liu, YL,et al. A peptidoglycan recognition protein involved in immune recognition and immune defenses in Ruditapes philippinarum[J]. FISH & SHELLFISH IMMUNOLOGY,2019,88:441-448.
APA Yang, DL.,Han, YJ.,Liu, YL.,Cao, RW.,Wang, Q.,...&Zhao, JM.(2019).A peptidoglycan recognition protein involved in immune recognition and immune defenses in Ruditapes philippinarum.FISH & SHELLFISH IMMUNOLOGY,88,441-448.
MLA Yang, DL,et al."A peptidoglycan recognition protein involved in immune recognition and immune defenses in Ruditapes philippinarum".FISH & SHELLFISH IMMUNOLOGY 88(2019):441-448.
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