| Scavenger receptor class B type I (SR-BI) in Ruditapes philippinarum: A versatile receptor with multiple functions | |
Yang, DL ; Han, YJ; Chen, LZ; Liu, YL; Cao, RW; Wang, Q; Zhao, JM
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| 发表期刊 | FISH & SHELLFISH IMMUNOLOGY
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| ISSN | 1050-4648 |
| 2019-05 | |
| 卷号 | 88页码:328-334 |
| 关键词 | Ruditapes philippinarum Scavenger receptor Pattern recognition receptor Immune recognition |
| 研究领域 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| DOI | 10.1016/j.fsi.2019.03.009 |
| 产权排序 | [Yang, Dinglong; Han, Yijing; Liu, Yongliang; Cao, Ruiwen; Wang, Qing; Zhao, Jianmin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Muping Coastal Environm Res Stn, Yantai 264003, Peoples R China; [Yang, Dinglong; Wang, Qing; Zhao, Jianmin] Chinese Acad Sci, Res & Dev Ctr Efficient Utilknt Coastal Bioresour, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China; [Zhao, Jianmin] Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Shandong, Peoples R China; [Han, Yijing; Cao, Ruiwen] Univ Chinese Acad Sci, Beijing 100049, Peoples R China; [Chen, Lizhu] Shandong Marine Resource & Environm Res Inst, Yantai 264006, Peoples R China |
| 通讯作者 | Zhao, Jianmin(jmzhao@yic.ac.cn) |
| 作者部门 | 海岸带生物资源高效利用研究与发展中心 |
| 英文摘要 | In the present study, a scavenger receptor class B type I (designed as RpSR-BI) was cloned and characterized from manila clam Ruditapes philippinarum. The full-length cDNA of RpSR-BI was of 2000 bp, containing an open reading frame (ORF) of 1515 bp. Multiple alignments and phylogenetic analysis strongly suggested that RpSR-BI was a member of the scavenger receptors family. The mRNA transcript of RpSR-BI was constitutively expressed in all tested tissues, and mainly expressed in hepatopancreas and hemocytes. Generally, Vihrio anguillanon or Micrococcus luteus challenge induced the expression of RpSR-BI transcripts in hemocytes of manila clams. Recombinant protein of RpSR-BI (rRpSR-BI) could bind lipopolysaccharides, peptidoglycan and glucan, but not chitin in vitro. Coinciding with the PAMPs binding assay, a broad agglutination spectrum was displayed by rRpSR-BI including Gram-positive bacteria and Gram-negative bacteria. Moreover, rRpSR-BI could enhance the phagocytosis and chemotaxis of hemocytes. These results showed that RpSR-BI functioned as a pattern recognition receptor (PRR) with distinct recognition spectrum, and also as an opsonin involved in the innate immune response of R. philippinarum. |
| 文章类型 | Article |
| 资助机构 | Strategic Priority Research Program of the Chinese Academy of SciencesChinese Academy of Sciences [XDA23050303] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China [41806196] ; Key Research Program of the Chinese Academy of SciencesChinese Academy of Sciences [KFJ-STS-ZDTP-023] ; Two-Hundred Talents Plan of Yantai [Y839081021] ; Youth Innovation Promotion Association, CAS [2016196] |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | C-TYPE LECTIN ; PROTEIN ; RECOGNITION ; PEPTIDOGLYCAN ; EXPRESSION ; ADHESION ; CLONING ; ACTS |
| 研究领域[WOS] | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| WOS记录号 | WOS:000465060500039 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/24947 |
| 专题 | 海岸带生物学与生物资源利用重点实验室_海岸带生物资源高效利用研究与发展中心 海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室 支撑部门_中国科学院牟平海岸带环境综合试验站 |
| 作者单位 | 1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Muping Coastal Environm Res Stn, Yantai 264003, Peoples R China; 2.Chinese Acad Sci, Res & Dev Ctr Efficient Utilknt Coastal Bioresour, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China; 3.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Shandong, Peoples R China; 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China; 5.Shandong Marine Resource & Environm Res Inst, Yantai 264006, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yang, DL,Han, YJ,Chen, LZ,et al. Scavenger receptor class B type I (SR-BI) in Ruditapes philippinarum: A versatile receptor with multiple functions[J]. FISH & SHELLFISH IMMUNOLOGY,2019,88:328-334. |
| APA | Yang, DL.,Han, YJ.,Chen, LZ.,Liu, YL.,Cao, RW.,...&Zhao, JM.(2019).Scavenger receptor class B type I (SR-BI) in Ruditapes philippinarum: A versatile receptor with multiple functions.FISH & SHELLFISH IMMUNOLOGY,88,328-334. |
| MLA | Yang, DL,et al."Scavenger receptor class B type I (SR-BI) in Ruditapes philippinarum: A versatile receptor with multiple functions".FISH & SHELLFISH IMMUNOLOGY 88(2019):328-334. |
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