Highly sensitive detection of prostate cancer specific PCA3 mimic DNA using SERS-based competitive lateral flow assay | |
Fu, XL; Wen, JH; Li, JW; Lin, H; Liu, YM; Zhuang, XM; Tian, CY; Chen, LX | |
发表期刊 | NANOSCALE |
ISSN | 2040-3364 |
2019-09-07 | |
卷号 | 11期号:33页码:15530-15536 |
关键词 | POLYMERASE-CHAIN-REACTION GOLD NANOPARTICLES STRIP BIOSENSOR PCR AMPLIFICATION QUANTIFICATION IMMUNOASSAY SUBSTRATE STRATEGY BINDING |
研究领域 | Chemistry, Multidisciplinary ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary ; Physics, Applied |
DOI | 10.1039/c9nr04864b |
产权排序 | [Fu, Xiuli; Wen, Jiahui; Li, Jingwen; Lin, Hao; Liu, Yongming; Zhuang, Xuming; Tian, Chunyuan] Yantai Univ, Sch Chem & Chem Engn, Yantai 264005, Peoples R China; [Chen, Lingxin] Chinese Acad Sci, Res Ctr Coastal Environm Engn & Technol, Yantai Inst Coastal Zone Res, CAS Key Lab Coastal Environm Pocesses & Ecol Reme, Yantai 264003, Peoples R China; [Chen, Lingxin] Pilot Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Shandong, Peoples R China; [Chen, Lingxin] Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Shandong, Peoples R China |
通讯作者 | Liu, Yongming(liuyongming100@126.com) ; Chen, Lingxin(lxchen@yic.ac.cn) |
作者部门 | 海岸带环境工程技术研究与发展中心 |
英文摘要 | Accurate analysis of prostate cancer specific biomarkers plays an important role in the early diagnosis of prostate cancer. Traditional colorimetric lateral flow assay (LFA) has the limitations of low detection sensitivity and qualitative or semiquantitative detection. In this study, we developed a novel surface-enhanced Raman scattering (SERS)-based competitive LFA for the rapid and highly sensitive quantitative evaluation of prostate cancer antigen 3 (PCA3) mimic DNA. Herein, the competitive hybridization interaction with capture DNA between target PCA3 mimic DNA and reporter DNA-labeled SERS nanotags results in a change in the amount of SERS nanotags on the test line. The quantitative analysis of target PCA3 mimic DNA was realized by monitoring the Raman peak intensity of SERS nanotags on the test line. The limit of detection of PCA3 mimic DNA was estimated to be 3 fM, which is about three orders of magnitude more sensitive than that of a commercially available kit. By combining the outstanding characteristics of the well-established SERS-based competitive strategy and LFA platform, our design has strong potential for the early diagnosis of prostate cancer and other diseases. |
文章类型 | Article |
资助机构 | National Natural Science Foundation of ChinaNational Natural Science Foundation of China [21705139, 21575159, 21778047] ; National Natural Science Foundation of Shandong Province in China [ZR2017BB026] |
收录类别 | SCI |
语种 | 英语 |
关键词[WOS] | POLYMERASE-CHAIN-REACTION ; GOLD NANOPARTICLES ; STRIP BIOSENSOR ; PCR ; AMPLIFICATION ; QUANTIFICATION ; IMMUNOASSAY ; SUBSTRATE ; STRATEGY ; BINDING |
研究领域[WOS] | Chemistry, Multidisciplinary ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary ; Physics, Applied |
WOS记录号 | WOS:000484345500008 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.yic.ac.cn/handle/133337/24860 |
专题 | 中国科学院海岸带环境过程与生态修复重点实验室_海岸带环境工程技术研究与发展中心 中国科学院海岸带环境过程与生态修复重点实验室 |
作者单位 | 1.Yantai Univ, Sch Chem & Chem Engn, Yantai 264005, Peoples R China; 2.Chinese Acad Sci, Res Ctr Coastal Environm Engn & Technol, Yantai Inst Coastal Zone Res, CAS Key Lab Coastal Environm Pocesses & Ecol Reme, Yantai 264003, Peoples R China; 3.Pilot Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Shandong, Peoples R China; 4.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Shandong, Peoples R China |
推荐引用方式 GB/T 7714 | Fu, XL,Wen, JH,Li, JW,et al. Highly sensitive detection of prostate cancer specific PCA3 mimic DNA using SERS-based competitive lateral flow assay[J]. NANOSCALE,2019,11(33):15530-15536. |
APA | Fu, XL.,Wen, JH.,Li, JW.,Lin, H.,Liu, YM.,...&Chen, LX.(2019).Highly sensitive detection of prostate cancer specific PCA3 mimic DNA using SERS-based competitive lateral flow assay.NANOSCALE,11(33),15530-15536. |
MLA | Fu, XL,et al."Highly sensitive detection of prostate cancer specific PCA3 mimic DNA using SERS-based competitive lateral flow assay".NANOSCALE 11.33(2019):15530-15536. |
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