YIC-IR  > 中科院海岸带环境过程与生态修复重点实验室
Coordinated Regulation of the Size and Number of Polyhydroxybutyrate Granules by Core and Accessory Phasins in the Facultative Microsymbiont Sinorhizobium fredii NGR234
Sun, YW; Li, Y; Hu, Y; Chen, WX; Tian, CF
Source PublicationAPPLIED AND ENVIRONMENTAL MICROBIOLOGY
ISSN0099-2240
2019-10
Volume85Issue:19Pages:e00717-19
KeywordPHB legume phasin rhizobium symbiosis
MOST Discipline CatalogueBiotechnology & Applied Microbiology ; Microbiology
DOI10.1128/AEM.00717-19
Contribution Rank[Sun, Yan-Wei; Hu, Yue; Chen, Wen-Xin; Tian, Chang-Fu] China Agr Univ, Coll Biol Sci, State Key Lab Agrobiotechnol, Beijing, Peoples R China; [Sun, Yan-Wei; Hu, Yue; Chen, Wen-Xin; Tian, Chang-Fu] China Agr Univ, Coll Biol Sci, MOA Key Lab Soil Microbiol, Beijing, Peoples R China; [Sun, Yan-Wei; Hu, Yue; Chen, Wen-Xin; Tian, Chang-Fu] China Agr Univ, Coll Biol Sci, Rhizobium Res Ctr, Beijing, Peoples R China; [Li, Yan] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Utilizat, Yantai, Peoples R China
Department中国科学院海岸带环境过程重点实验室
AbstractThe exact roles of various granule-associated proteins (GAPs) of polyhydroxybutyrate (PHB) are poorly investigated, particularly for bacteria associated with plants. In this study, four structural GAPs, named phasins PhaP1 to PhaP4, were identified and demonstrated as true phasins colocalized with PHB granules in Sinorhizobium fredii NGR234, a facultative microsymbiont of Vigna unguiculata and many other legumes. The conserved PhaP2 dominated in regulation of granule size under both free-living and symbiotic conditions. PhaP1, another conserved phasin, made a higher contribution than accessory phasins PhaP4 and PhaP3 to PHB biosynthesis at stationary phase. PhaP3, with limited phyletic distribution on the symbiosis plasmid of Sinorhizobium, was more important than PhaP1 in regulating PHB biosynthesis in V. unguiculata nodules. Under the test conditions, no significant symbiotic defects were observed for mutants lacking individual or multiple phaP genes. The mutant lacking two PHB synthases showed impaired symbiotic performance, while mutations in individual PHB synthases or a PHB depolymerase yielded no symbiotic defects. This phenomenon is not related to either the number or size of PHB granules in test mutants within nodules. Distinct metabolic profiles and cocktail pools of GAPs of different phaP mutants imply that core and accessory phasins can be differentially involved in regulating other cellular processes in the facultative microsymbiont S. fredii NGR234. IMPORTANCE Polyhydroxybutyrate (PHB) granules are a store of carbon and energy in bacteria and archaea and play an important role in stress adaptation. Recent studies have highlighted distinct roles of several granule-associated proteins (GAPS) in regulating the size, number, and localization of PHB granules in free-living bacteria, though our knowledge of the role of GAPs in bacteria associated with plants is still limited. Here we report distinct roles of core and accessory phasins associated with PHB granules of Sinorhizobium fredii NGR234, a broad-host-range microsymbiont of diverse legumes. Core phasins PhaP2 and PhaP1 are conserved major phasins in free-living cells. PhaP2 and accessory phasin PhaP3, encoded by an auxiliary gene on the symbiosis plasmid, are major phasins in nitrogen-fixing bacteroids in cowpea nodules. GAPs and metabolic profiles can vary in different phaP mutants. Contrasting symbiotic performances between mutants lacking PHB synthases, depolymerase, or phasins were revealed.
SubtypeArticle
Indexed BySCI
Language英语
WOS KeywordPOLY-BETA-HYDROXYBUTYRATE ; POLY-3-HYDROXYBUTYRATE PHB ; PROTEIN ; POLY(3-HYDROXYBUTYRATE) ; SYMBIOSIS ; MELILOTI ; SYSTEM ; BROAD ; GENE ; IDENTIFICATION
WOS Research AreaBiotechnology & Applied Microbiology ; Microbiology
WOS IDWOS:000488849800003
Funding OrganizationNational Natural Science Foundation of ChinaNational Natural Science Foundation of China [31522003] ; National Basic Research Program of ChinaNational Basic Research Program of China [2015CB158301] ; Innovative Project of State Key Laboratory of Agrobiotechnology [2018SKLAB1-2]
Citation statistics
Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.yic.ac.cn/handle/133337/24848
Collection中科院海岸带环境过程与生态修复重点实验室
Affiliation1.China Agr Univ, Coll Biol Sci, State Key Lab Agrobiotechnol, Beijing, Peoples R China;
2.China Agr Univ, Coll Biol Sci, MOA Key Lab Soil Microbiol, Beijing, Peoples R China;
3.China Agr Univ, Coll Biol Sci, Rhizobium Res Ctr, Beijing, Peoples R China;
4.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Utilizat, Yantai, Peoples R China
Recommended Citation
GB/T 7714
Sun, YW,Li, Y,Hu, Y,et al. Coordinated Regulation of the Size and Number of Polyhydroxybutyrate Granules by Core and Accessory Phasins in the Facultative Microsymbiont Sinorhizobium fredii NGR234[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY,2019,85(19):e00717-19.
APA Sun, YW,Li, Y,Hu, Y,Chen, WX,&Tian, CF.(2019).Coordinated Regulation of the Size and Number of Polyhydroxybutyrate Granules by Core and Accessory Phasins in the Facultative Microsymbiont Sinorhizobium fredii NGR234.APPLIED AND ENVIRONMENTAL MICROBIOLOGY,85(19),e00717-19.
MLA Sun, YW,et al."Coordinated Regulation of the Size and Number of Polyhydroxybutyrate Granules by Core and Accessory Phasins in the Facultative Microsymbiont Sinorhizobium fredii NGR234".APPLIED AND ENVIRONMENTAL MICROBIOLOGY 85.19(2019):e00717-19.
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