Metabolic engineering of Synechocystis sp. PCC6803 to produce astaxanthin

doi:10.1016/j.algal.2019.101679

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	Metabolic engineering of Synechocystis sp. PCC6803 to produce astaxanthin
	Liu, YM ; Cui, YL; Chen, J ; Qin, S; Chen, G
发表期刊	ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
ISSN	2211-9264
	2019-12
卷号	44 页码:UNSP 101679
关键词	Synechocystis sp. PCC6803 Astaxanthin beta-Carotene ketolase (bkt) Carotenoid hydroxylase (crtR-B) Metabolic engineering
研究领域	Biotechnology & Applied Microbiology
DOI	10.1016/j.algal.2019.101679
产权排序	[Liu, Yaming; Cui, Yulin; Chen, Jun; Qin, Song] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Biol Resource Utilizat, Yantai 264003, Shandong, Peoples R China; [Liu, Yaming] Univ Chinese Acad Sci, Beijing 101418, Peoples R China; [Chen, Gao] Shandong Acad Agr Sci, Biotechnol Res Ctr, Jinan 250100, Shandong, Peoples R China; [Chen, Gao] Shandong Prov Key Lab Acad Crop Genet Improvement, Jinan 250100, Shandong, Peoples R China
通讯作者	Qin, Song(sqin@yic.ac.cn) ; Chen, Gao(gxchen001@hotmail.com)
作者部门	海岸带生物学与生物资源保护实验室
英文摘要	Astaxanthin is a carotenoid with wide applications because of its strong antioxidant properties. It is mainly produced from the green alga Haematococcus pluvialis, but large-scale production remains a challenge. To provide another source, here, Synechocystis sp. PCC6803 was adopted as the chassis cell, and its carotenoid biosynthesis pathway was genetically extended to produce astaxanthin. The beta-carotene ketolase gene (bkt) and carotenoid hydroxylase gene (crtR-B) from H. pluvialis, which wild-type Synechocystis sp. PCC6803 lacks, were optimized based on the codon preference of Synechocystis sp. PCC6803 and heterologously expressed in that bacterium to create a strain capable of producing astaxanthin. The Synechocystis sp. PCC6803 mutant synthesized 4.81 +/- 0.06 mg astaxanthin per gram of cells (dry weight) after nitrogen starvation for 14 days. More than 30% of the total astaxanthin was in the free form, and 82.61% was the strong antioxidant 3S, 3'S-astaxanthin isoform. These results show the industrial application potential of metabolically-engineered Synechocystis sp. PCC6803 for astaxanthin production.
文章类型	Article
资助机构	National Key PD Program [2016YFE0106700] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China [41876188] ; Natural Science Foundation of Shandong Province, ChinaNatural Science Foundation of Shandong Province [ZR2018ZB0210, ZR2016CM48] ; Key Research and Development Program of Shandong [2018GSF121019, 2019GSF107098] ; Project of Innovation & Development of Marine Economy [HHCL201803] ; seed project of Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences [YIC755031013] ; Science and Technology Service Network Initiative of the Chinese Academy of Sciences [KFJ-STS-ZDTP-023]
收录类别	SCI
语种	英语
关键词[WOS]	HAEMATOCOCCUS-PLUVIALIS ; CRTR-B ; MICROALGAE ; ACCUMULATION ; CYANOBACTERIA ; CAROTENOIDS ; ISOMERS
研究领域[WOS]	Biotechnology & Applied Microbiology
WOS记录号	WOS:000498792900039
引用统计	被引频次：17[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/24805
专题	海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
作者单位	1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Biol Resource Utilizat, Yantai 264003, Shandong, Peoples R China; 2.Univ Chinese Acad Sci, Beijing 101418, Peoples R China; 3.Shandong Acad Agr Sci, Biotechnol Res Ctr, Jinan 250100, Shandong, Peoples R China; 4.Shandong Prov Key Lab Acad Crop Genet Improvement, Jinan 250100, Shandong, Peoples R China
推荐引用方式 GB/T 7714	Liu, YM,Cui, YL,Chen, J,et al. Metabolic engineering of Synechocystis sp. PCC6803 to produce astaxanthin[J]. ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,2019,44:UNSP 101679.
APA	Liu, YM,Cui, YL,Chen, J,Qin, S,&Chen, G.(2019).Metabolic engineering of Synechocystis sp. PCC6803 to produce astaxanthin.ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,44,UNSP 101679.
MLA	Liu, YM,et al."Metabolic engineering of Synechocystis sp. PCC6803 to produce astaxanthin".ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS 44(2019):UNSP 101679.

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